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2013 | OriginalPaper | Buchkapitel

Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales

verfasst von : Vincent Rouger, Cyrille Billaudeau, Tomasz Trombik, Sébastien Mailfert, Yannick Hamon, Hai-Tao He, Didier Marguet

Erschienen in: Fluorescent Methods to Study Biological Membranes

Verlag: Springer Berlin Heidelberg

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Abstract

The plasma membrane delineating the cell is a complex multicomponent assembly of lipids and proteins. Deciphering the lateral organization of this supramolecular complex on appropriate length and temporal scales is necessary to unravel its implication in biological function. Here, we show how measurements of diffusion may be taken to shed light on membrane function. We first describe the current methods used to report lateral diffusion of membrane components. We then focus on one fluorescent correlation spectroscopy (FCS)-based method, namely, the spot variation FCS (svFCS), which allows the characterization of the modes of molecular confinement within the plasma membrane of living cells. We next illustrate with different biological systems the progress made toward improving our understanding of cell membrane function. We also discuss the findings with regard to the current view of nanoscale domains/assemblies as a general membrane-organizing principle.

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Vorheriges Kapitel Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

Nächstes Kapitel STED-FCS Nanoscopy of Membrane Dynamics

Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175(23):720–731CrossRef

Singer SJ (2004) Some early history of membrane molecular biology. Annu Rev Physiol 66:1–27. doi:10.1146/annurev.physiol.66.032902.131835 CrossRef

van Meer G (2005) Cellular lipidomics. EMBO J 24(18):3159–3165. doi:10.1038/sj.emboj.7600798 CrossRef

Binda C, Newton-Vinson P, Hubalek F, Edmondson DE, Mattevi A (2002) Structure of human monoamine oxidase b, a drug target for the treatment of neurological disorders. Nat Struct Biol 9(1):22–26. doi:10.1038/nsb732 CrossRef

Engelman DM (2005) Membranes are more mosaic than fluid. Nature 438(7068):578–580CrossRef

Bracey MH, Hanson MA, Masuda KR, Stevens RC, Cravatt BF (2002) Structural adaptations in a membrane enzyme that terminates endocannabinoid signaling. Science 298(5599):1793–1796. doi:10.1126/science.1076535 CrossRef

Lingwood D, Simons K (2010) Lipid rafts as a membrane-organizing principle. Science 327(5961):46–50. doi:10.1126/science.1174621 CrossRef

Bagatolli LA, Ipsen JH, Simonsen A, Mouritsen OG (2010) An outlook on organization of lipids in membranes: searching for a realistic connection with the organization of biological membranes. Prog Lipid Res 49(4):378–467CrossRef

Munro S (2003) Lipid rafts: elusive or illusive? Cell 115(4):377–388CrossRef

10.

Simons K, Gerl MJ (2010) Revitalizing membrane rafts: new tools and insights. Nat Rev Mol Cell Biol 11(10):688–699. doi:10.1038/nrm2977 CrossRef

11.

Saxton MJ, Jacobson K (1997) Single-particle tracking: applications to membrane dynamics. Annu Rev Biophys Biomol Struct 26:373–399. doi:10.1146/annurev.biophys.26.1.373 CrossRef

12.

Schutz GJ, Kada G, Pastushenko VP, Schindler H (2000) Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J 19(5):892–901. doi:10.1093/emboj/19.5.892 CrossRef

13.

Sako Y, Minoghchi S, Yanagida T (2000) Single-molecule imaging of egfr signalling on the surface of living cells. Nat Cell Biol 2(3):168–172. doi:10.1038/35004044 CrossRef

14.

Schmidt T, Schutz GJ, Baumgartner W, Gruber HJ, Schindler H (1996) Imaging of single molecule diffusion. Proc Natl Acad Sci USA 93(7):2926–2929CrossRef

15.

Kalaidzidis Y (2007) Intracellular objects tracking. Eur J Cell Biol 86(9):569–578CrossRef

16.

Levi V, Ruan Q, Gratton E (2005) 3-d particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells. Biophys J 88(4):2919–2928. doi:10.1529/biophysj.104.044230 CrossRef

17.

Wieser S, Schutz GJ (2008) Tracking single molecules in the live cell plasma membrane-do’s and don’t’s. Methods 46(2):131–140. doi:10.1016/j.ymeth.2008.06.010 CrossRef

18.

Serge A, Bertaux N, Rigneault H, Marguet D (2008) Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes. Nat Methods 5(8):687–694. doi:10.1038/nmeth.1233 CrossRef

19.

Kao HP, Verkman AS (1994) Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position. Biophys J 67(3):1291–1300. doi:10.1016/S0006-3495(94)80601-0 CrossRef

20.

Holtzer L, Meckel T, Schmidt T (2007) Nanometric three-dimensional tracking of individual quantum dots in cells. Appl Phys Lett 90(5):053902–053903CrossRef

21.

Chen Y, Lagerholm BC, Yang B, Jacobson K (2006) Methods to measure the lateral diffusion of membrane lipids and proteins. Methods 39(2):147–153. doi:10.1016/j.ymeth.2006.05.008 CrossRef

22.

Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J 16(9):1055–1069. doi:10.1016/S0006-3495(76)85755-4 CrossRef

23.

Soumpasis DM (1983) Theoretical analysis of fluorescence photobleaching recovery experiments. Biophys J 41(1):95–97. doi:10.1016/S0006-3495(83)84410-5 CrossRef

24.

Oancea E, Teruel MN, Quest AF, Meyer T (1998) Green fluorescent protein (gfp)-tagged cysteine-rich domains from protein kinase c as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol 140(3):485–498CrossRef

25.

Siggia ED, Lippincott-Schwartz J, Bekiranov S (2000) Diffusion in inhomogeneous media: theory and simulations applied to whole cell photobleach recovery. Biophys J 79(4):1761–1770CrossRef

26.

Elson EL, Magde D (1974) Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers 13(1):1–27CrossRef

27.

Magde D, Elson EL, Webb WW (1974) Fluorescence correlation spectroscopy. Ii. An experimental realization. Biopolymers 13(1):29–61CrossRef

28.

Chen H, Farkas ER, Webb WW (2008) Chapter 1: In vivo applications of fluorescence correlation spectroscopy. Methods Cell Biol 89:3–35. doi:10.1016/S0091-679X(08)00601-8 CrossRef

29.

Haustein E, Schwille P (2007) Fluorescence correlation spectroscopy: novel variations of an established technique. Annu Rev Biophys Biomol Struct 36:151–169. doi:10.1146/annurev.biophys.36.040306.132612 CrossRef

30.

Bacia K, Kim SA, Schwille P (2006) Fluorescence cross-correlation spectroscopy in living cells. Nat Methods 3(2):83–89CrossRef

31.

Chiantia S, Ries J, Schwille P (2009) Fluorescence correlation spectroscopy in membrane structure elucidation. Biochim Biophys Acta 1788(1):225–233. doi:10.1016/j.bbamem.2008.08.013 CrossRef

32.

He HT, Marguet D (2011) Detecting nanodomains in living cell membrane by fluorescence correlation spectroscopy. Annu Rev Phys Chem 62:417–436. doi:10.1146/annurev-physchem-032210-103402 CrossRef

33.

Digman MA, Gratton E (2011) Lessons in fluctuation correlation spectroscopy. Annu Rev Phys Chem 62:645–668. doi:10.1146/annurev-physchem-032210-103424 CrossRef

34.

Schwille P, Meyer-Almes FJ, Rigler R (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72(4):1878–1886CrossRef

35.

Larson DR, Gosse JA, Holowka DA, Baird BA, Webb WW (2005) Temporally resolved interactions between antigen-stimulated ige receptors and lyn kinase on living cells. J Cell Biol 171(3):527–536CrossRef

36.

Lillemeier BF, Mortelmaier MA, Forstner MB, Huppa JB, Groves JT, Davis MM (2009) Tcr and lat are expressed on separate protein islands on t cell membranes and concatenate during activation. Nat Immunol 11(1):90–96. doi:ni.1832 [pii]. 10.1038/ni.1832 CrossRef

37.

Delon A, Derouard J, Delapierre G, Jaffiol R (2006) Measurement of surface concentration of fluorophores by fluorescence fluctuation spectroscopy. Opt Lett 31(8):1142–1144CrossRef

38.

Jaffiol R, Blancquaert Y, Delon A, Derouard J (2006) Spatial fluorescence cross-correlation spectroscopy. Appl Opt 45(6):1225–1235CrossRef

39.

Ries J, Schwille P (2006) Studying slow membrane dynamics with continuous wave scanning fluorescence correlation spectroscopy. Biophys J 91(5):1915–1924. doi:10.1529/biophysj.106.082297 CrossRef

40.

Ruan Q, Cheng MA, Levi M, Gratton E, Mantulin WW (2004) Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (sfcs). Biophys J 87(2):1260–1267CrossRef

41.

Petrasek Z, Schwille P (2008) Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy. Biophys J 94(4):1437–1448. doi:10.1529/biophysj.107.108811 CrossRef

42.

Dertinger T, Pacheco V, von der Hocht I, Hartmann R, Gregor I, Enderlein J (2007) Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements. Chemphyschem 8(3):433–443. doi:10.1002/cphc.200600638 CrossRef

43.

Dertinger T, Loman A, Ewers B, Muller CB, Kramer B, Enderlein J (2008) The optics and performance of dual-focus fluorescence correlation spectroscopy. Opt Express 16(19):14353–14368. doi:171559 [pii]CrossRef

44.

Humpolickova J, Gielen E, Benda A, Fagulova V, Vercammen J, Vandeven M, Hof M, Ameloot M, Engelborghs Y (2006) Probing diffusion laws within cellular membranes by z-scan fluorescence correlation spectroscopy. Biophys J 91(3):L23–25. doi:10.1529/biophysj.106.089474 CrossRef

45.

Kusumi A, Shirai YM, Koyama-Honda I, Suzuki KG, Fujiwara TK (2010) Hierarchical organization of the plasma membrane: investigations by single-molecule tracking vs fluorescence correlation spectroscopy. FEBS Lett 584(9):1814–1823. doi:10.1016/j.febslet.2010.02.047 CrossRef

46.

Kusumi A, Nakada C, Ritchie K, Murase K, Suzuki K, Murakoshi H, Kasai RS, Kondo J, Fujiwara T (2005) Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules. Annu Rev Biophys Biomol Struct 34:351–378CrossRef

47.

Meseth U, Wohland T, Rigler R, Vogel H (1999) Resolution of fluorescence correlation measurements. Biophys J 76(3):1619–1631. doi:10.1016/S0006-3495(99)77321-2 CrossRef

48.

Bouchaud J-P, Georges A (1990) Anomalous diffusion in disordered media: statistical mechanisms, models and physical applications. Phys Reports 195(4–5):127–293CrossRef

49.

Saxton MJ (2007) A biological interpretation of transient anomalous subdiffusion. I. Qualitative model. Biophys J 92(4):1178–1191. doi:10.1529/biophysj.106.092619 CrossRef

50.

Saxton MJ (2008) A biological interpretation of transient anomalous subdiffusion. Ii. Reaction kinetics. Biophys J 94(3):760–771. doi:10.1529/biophysj.107.114074 CrossRef

51.

Wawrezinieck L, Rigneault H, Marguet D, Lenne PF (2005) Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization. Biophys J 89(6):4029–4042. doi:10.1529/biophysj.105.067959 CrossRef

52.

Lenne PF, Wawrezinieck L, Conchonaud F, Wurtz O, Boned A, Guo XJ, Rigneault H, He HT, Marguet D (2006) Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork. EMBO J 25(14):3245–3256. doi:10.1038/sj.emboj.7601214 CrossRef

53.

Wawrezinieck L, Lenne P-F, Marguet D, Rigneault H (2004) Fluorescence correlation spectroscopy to determine diffusion laws: application to live cell membranes. Proc SPIE 5462:92–102CrossRef

54.

Saxton MJ (2005) New and notable: fluorescence correlation spectroscopy. Biophys J 89(6):3678–3679CrossRef

55.

Billaudeau C, Mailfert S, Trombik T, Bertaux N, Rouger V, Hamon Y, He HT, Marguet D (2012) Probing the plasma membrane organization in living cells by spot variation fluorescence correlation spectroscopy. Methods Enzymol (in press)

56.

Destainville N (2008) Theory of fluorescence correlation spectroscopy at variable observation area for two-dimensional diffusion on a meshgrid. Soft Matter 4(6):1288–1301CrossRef

57.

Ruprecht V, Wieser S, Marguet D, Schutz GJ (2011) Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes. Biophys J 100(11):2839–2845. doi:10.1016/j.bpj.2011.04.035 CrossRef

58.

Wenger J, Conchonaud F, Dintinger J, Wawrezinieck L, Ebbesen TW, Rigneault H, Marguet D, Lenne PF (2007) Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization. Biophys J 92(3):913–919. doi:10.1529/biophysj.106.096586 CrossRef

59.

Manzo C, van Zanten TS, Garcia-Parajo MF (2011) Nanoscale fluorescence correlation spectroscopy on intact living cell membranes with nsom probes. Biophys J 100(2):L8–10. doi:10.1016/j.bpj.2010.12.3690 CrossRef

60.

Eggeling C, Ringemann C, Medda R, Schwarzmann G, Sandhoff K, Polyakova S, Belov VN, Hein B, von Middendorff C, Schonle A, Hell SW (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457(7233):1159–1162. doi:10.1038/nature07596 CrossRef

61.

Mueller V, Ringemann C, Honigmann A, Schwarzmann G, Medda R, Leutenegger M, Polyakova S, Belov VN, Hell SW, Eggeling C (2011) Sted nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells. Biophys J 101(7):1651–1660. doi:10.1016/j.bpj.2011.09.006 CrossRef

62.

Cahuzac N, Baum W, Kirkin V, Conchonaud F, Wawrezinieck L, Marguet D, Janssen O, Zornig M, Hueber AO (2006) Fas ligand is localized to membrane rafts, where it displays increased cell death-inducing activity. Blood 107(6):2384–2391. doi:10.1182/blood-2005-07-2883 CrossRef

63.

Guardiola-Serrano F, Rossin A, Cahuzac N, Luckerath K, Melzer I, Mailfert S, Marguet D, Zornig M, Hueber AO (2010) Palmitoylation of human fasl modulates its cell death-inducing function. Cell Death Disease 1:e88. doi:10.1038/cddis.2010.62 CrossRef

64.

Conchonaud F, Nicolas S, Amoureux MC, Menager C, Marguet D, Lenne PF, Rougon G, Matarazzo V (2007) Polysialylation increases lateral diffusion of neural cell adhesion molecule in the cell membrane. J Biol Chem 282(36):26266–26274. doi:10.1074/jbc.M608590200 CrossRef

65.

Chakrabandhu K, Herincs Z, Huault S, Dost B, Peng L, Conchonaud F, Marguet D, He HT, Hueber AO (2007) Palmitoylation is required for efficient fas cell death signaling. EMBO J 26(1):209–220. doi:10.1038/sj.emboj.7601456 CrossRef

66.

Lasserre R, Guo XJ, Conchonaud F, Hamon Y, Hawchar O, Bernard AM, Soudja SM, Lenne PF, Rigneault H, Olive D, Bismuth G, Nunes JA, Payrastre B, Marguet D, He HT (2008) Raft nanodomains contribute to akt/pkb plasma membrane recruitment and activation. Nat Chem Biol 4(9):538–547. doi:10.1038/nchembio.103 CrossRef

67.

Chakrabandhu K, Huault S, Garmy N, Fantini J, Stebe E, Mailfert S, Marguet D, Hueber AO (2008) The extracellular glycosphingolipid-binding motif of fas defines its internalization route, mode and outcome of signals upon activation by ligand. Cell Death Differ 15(12):1824–1837. doi:10.1038/cdd.2008.115 CrossRef

68.

Rossin A, Kral R, Lounnas N, Chakrabandhu K, Mailfert S, Marguet D, Hueber AO (2010) Identification of a lysine-rich region of fas as a raft nanodomain targeting signal necessary for fas-mediated cell death. Exp Cell Res 316(9):1513–1522. doi:10.1016/j.yexcr.2010.03.002 CrossRef

69.

Guia S, Jaeger BN, Piatek S, Mailfert S, Trombik T, Fenis A, Chevrier N, Walzer T, Kerdiles YM, Marguet D, Vivier E, Ugolini S (2011) Confinement of activating receptors at the plasma membrane controls natural killer cell tolerance. Sci Signal 4 (167):ra21. doi:10.1126/scisignal.2001608

70.

Sako Y, Kusumi A (1995) Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether. J Cell Biol 129(6):1559–1574CrossRef

71.

Morone N, Fujiwara T, Murase K, Kasai RS, Ike H, Yuasa S, Usukura J, Kusumi A (2006) Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. J Cell Biol 174(6):851–862. doi:10.1083/jcb.200606007 CrossRef

72.

Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J (2010) Interleukin-7 compartmentalizes its receptor signaling complex to initiate cd4 t lymphocyte response. J Biol Chem 285(20):14898–14908. doi:10.1074/jbc.M110.104232 CrossRef

73.

Ganguly S, Chattopadhyay A (2010) Cholesterol depletion mimics the effect of cytoskeletal destabilization on membrane dynamics of the serotonin1a receptor: A zfcs study. Biophys J 99(5):1397–1407. doi:10.1016/j.bpj.2010.06.031 CrossRef

74.

Kiessling V, Crane JM, Tamm LK (2006) Transbilayer effects of raft-like lipid domains in asymmetric planar bilayers measured by single molecule tracking. Biophys J 91(9):3313–3326. doi:10.1529/biophysj.106.091421 CrossRef

Titel: Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales
verfasst von: Vincent Rouger
Cyrille Billaudeau
Tomasz Trombik
Sébastien Mailfert
Yannick Hamon
Hai-Tao He
Didier Marguet
Verlag: Springer Berlin Heidelberg
Buch: Fluorescent Methods to Study Biological Membranes
Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright-Jahr: 2013
DOI: https://doi.org/10.1007/4243_2012_56

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