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2013 | OriginalPaper | Buchkapitel

Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

verfasst von : Monika Zelman-Femiak, Yamunadevi Subburaj, Ana J. García-Sáez

Erschienen in: Fluorescent Methods to Study Biological Membranes

Verlag: Springer Berlin Heidelberg

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Abstract

This chapter describes the application of fluorescence correlation spectroscopy (FCS) as a powerful technique for the study of membrane organization and interactions. Monitoring the fluorescence signal fluctuations allows resolving concentrations, diffusion coefficients, and binding of several membrane components in experiments in vitro as well as in vivo.

We discuss the basic principles of FCS and explain novel implementations of FCS introduced to overcome the technical difficulties present in the standard version of fluorescence correlation spectroscopy. Finally, we report several examples of studies with the application of FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization and membrane interactions.

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Vorheriges Kapitel Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)

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Titel: Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions
verfasst von: Monika Zelman-Femiak
Yamunadevi Subburaj
Ana J. García-Sáez
Verlag: Springer Berlin Heidelberg
Buch: Fluorescent Methods to Study Biological Membranes
Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright-Jahr: 2013
DOI: https://doi.org/10.1007/4243_2012_49

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