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Erschienen in:
2017 | OriginalPaper | Buchkapitel
Detremination of kinetic effect of Metoprolol and Ranitidine on HRP- modified GC electrode biosensor
verfasst von : Zerina Bešić, Safija Herenda, Trivo Stanković, Jelena Ostojić
Erschienen in: CMBEBIH 2017
Verlag: Springer Singapore
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Peroxidase is classified as oxido reductive enzyme which catalyzes the oxidation of the substrate by a hydrogen peroxide solution, the composition of a protein which is the prosthetic group of HEM. Inthispaper,the effect of active substance from the different groups of drugs on peroxidase activity was studied. Electro chemical tests were performedinclassical three– electrode system with techniques of cyclic voltametry and chronoamperometry. Ampero metric biosensor for the determination of H2O2 is presented,based on GC electrode on which per oxidase is immobilized in Nafion film.Cyclic voltametry technique was used to research the influence of different substrate concentration on the enzyme activity in a potential range between – 1,0V and 0,7V and scanrate of 50mV/s.Chrono amperometric technique was used for the determination of kinetic parameters Km and Vmax ataconstant potential of 0,9V.