Development of a Pharmacological Test System in a Miniaturized Hollow Fiber Bioreactor

Pharmaceutical usage in vitro has a long proving history. Common drug response assays do not always correlate with in vivo-like drug resistance and sensitivity. In contrast, tree-dimensional tissue cultivation in miniaturized hollow fiber bioreactors (mHFBR) do, indicating that drug response is a function of tissue architecture 1. Establishing a test system in vitro, feasible for theurapeutic drug monitoring (TDM), will considerably improve individual patient care, especially in leukæmia therapy, e.g. B-CCL, CML. For detecting drug effects, suitable sensors have to be found, thereto the sensors have to be so small to enable the integration in the HFBR. We emphasize on monitoring interbolism parameter, e.g. direct oxygen consumption and the NAD(P)H content, utilizing optical microsensors. In the study, we present a successful drug monitoring on a human bone marrow long-time (14d) cultivation exposed to a new biopharmaceutical drug for clinical usage, GM-CSF in combination with IL-3.