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Erschienen in: Clean Technologies and Environmental Policy 7/2016

19.02.2016 | Original Paper

Docosahexaenoic acid production from crude glycerol by Schizochytrium limacinum SR21

verfasst von: Yun-Tsun Lung, Chung Hong Tan, Pau Loke Show, Tau Chuan Ling, John Chi-Wei Lan, Hon Loong Lam, Jo-Shu Chang

Erschienen in: Clean Technologies and Environmental Policy | Ausgabe 7/2016

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Abstract

In our search for a substitute energy source, microalgal biodiesel has presented itself as a potential candidate. However, the development of biodiesel results in the overproduction of crude glycerol, which can cause undesirable environmental issues. The environmental harm can be minimized by converting crude glycerol into value-added products. One solution involves using a microalga Schizochytrium limacinum SR21 to convert crude glycerol into docosahexaenoic acid (DHA). DHA is an essential fatty acid, necessary for developing brain functions in infants and maintaining healthy brain activity in adults. In our study, the highest DHA productivity of 233.73 mg/g biomass was obtained using 3 % crude glycerol in Medium 2 at 20 °C under mixo/heterotrophic cultivation.

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Literatur
Zurück zum Zitat Abad S, Turon X (2015) Biotechnological production of docosahexaenoic acid using aurantiochytrium limacinum: carbon sources comparison and growth characterization. Mar Drugs 13:7275–7284CrossRef Abad S, Turon X (2015) Biotechnological production of docosahexaenoic acid using aurantiochytrium limacinum: carbon sources comparison and growth characterization. Mar Drugs 13:7275–7284CrossRef
Zurück zum Zitat Athalye SK, Garcia RA, Wen Z (2009) Use of biodiesel-derived crude glycerol for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. J Agric Food Chem 57:2739–2744CrossRef Athalye SK, Garcia RA, Wen Z (2009) Use of biodiesel-derived crude glycerol for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. J Agric Food Chem 57:2739–2744CrossRef
Zurück zum Zitat Batista AP et al (2015) Combining urban wastewater treatment with biohydrogen production–an integrated microalgae-based approach. Bioresour Technol 184:230–235CrossRef Batista AP et al (2015) Combining urban wastewater treatment with biohydrogen production–an integrated microalgae-based approach. Bioresour Technol 184:230–235CrossRef
Zurück zum Zitat Bizzo WA, Lenço PC, Carvalho DJ, Veiga JPS (2014) The generation of residual biomass during the production of bio-ethanol from sugarcane, its characterization and its use in energy production. Renew Sustain Energy Rev 29:589–603CrossRef Bizzo WA, Lenço PC, Carvalho DJ, Veiga JPS (2014) The generation of residual biomass during the production of bio-ethanol from sugarcane, its characterization and its use in energy production. Renew Sustain Energy Rev 29:589–603CrossRef
Zurück zum Zitat Chacón-Lee T, González-Mariño G (2010) Microalgae for “healthy” foods—possibilities and challenges. Comprehensive Reviews in Food Science and Food Safety 9:655–675CrossRef Chacón-Lee T, González-Mariño G (2010) Microalgae for “healthy” foods—possibilities and challenges. Comprehensive Reviews in Food Science and Food Safety 9:655–675CrossRef
Zurück zum Zitat Chen Y-H, Walker TH (2011) Biomass and lipid production of heterotrophic microalgae Chlorella protothecoides by using biodiesel-derived crude glycerol. Biotechnol Lett 33:1973–1983CrossRef Chen Y-H, Walker TH (2011) Biomass and lipid production of heterotrophic microalgae Chlorella protothecoides by using biodiesel-derived crude glycerol. Biotechnol Lett 33:1973–1983CrossRef
Zurück zum Zitat Christien E, Matthias P, Maria B, Lolke S (2014) Microalgae-based products for the food and feed sector: an outlook for Europe. European Commission, Joint Research Centre, Institute for Prospective Technological Studies, Luxembourg Christien E, Matthias P, Maria B, Lolke S (2014) Microalgae-based products for the food and feed sector: an outlook for Europe. European Commission, Joint Research Centre, Institute for Prospective Technological Studies, Luxembourg
Zurück zum Zitat Ethier S, Woisard K, Vaughan D, Wen Z (2011) Continuous culture of the microalgae Schizochytrium limacinum on biodiesel-derived crude glycerol for producing docosahexaenoic acid. Bioresour Technol 102:88–93CrossRef Ethier S, Woisard K, Vaughan D, Wen Z (2011) Continuous culture of the microalgae Schizochytrium limacinum on biodiesel-derived crude glycerol for producing docosahexaenoic acid. Bioresour Technol 102:88–93CrossRef
Zurück zum Zitat Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues Journal of Biological Chemistry 226:497–509 Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues Journal of Biological Chemistry 226:497–509
Zurück zum Zitat Guedes A, Amaro HM, Malcata FX (2011) Microalgae as sources of high added-value compounds—a brief review of recent work. Biotechnol Prog 27:597–613CrossRef Guedes A, Amaro HM, Malcata FX (2011) Microalgae as sources of high added-value compounds—a brief review of recent work. Biotechnol Prog 27:597–613CrossRef
Zurück zum Zitat Hu S, Wan C, Li Y (2012) Production and characterization of biopolyols and polyurethane foams from crude glycerol based liquefaction of soybean straw. Bioresour Technol 103:227–233CrossRef Hu S, Wan C, Li Y (2012) Production and characterization of biopolyols and polyurethane foams from crude glycerol based liquefaction of soybean straw. Bioresour Technol 103:227–233CrossRef
Zurück zum Zitat Ikeda Y, Park EY, Okuda N (2006) Bioconversion of waste office paper to gluconic acid in a turbine blade reactor by the filamentous fungus Aspergillus niger. Bioresour Technol 97:1030–1035CrossRef Ikeda Y, Park EY, Okuda N (2006) Bioconversion of waste office paper to gluconic acid in a turbine blade reactor by the filamentous fungus Aspergillus niger. Bioresour Technol 97:1030–1035CrossRef
Zurück zum Zitat Johnson DT, Taconi KA (2007) The glycerin glut: options for the value-added conversion of crude glycerol resulting from biodiesel production. Environ Prog 26:338–348CrossRef Johnson DT, Taconi KA (2007) The glycerin glut: options for the value-added conversion of crude glycerol resulting from biodiesel production. Environ Prog 26:338–348CrossRef
Zurück zum Zitat Klassen V, Blifernez-Klassen O, Hoekzema Y, Mussgnug JH, Kruse O (2015) A novel one-stage cultivation/fermentation strategy for improved biogas production with microalgal biomass Journal of Biotechnology 215:44–51 Klassen V, Blifernez-Klassen O, Hoekzema Y, Mussgnug JH, Kruse O (2015) A novel one-stage cultivation/fermentation strategy for improved biogas production with microalgal biomass Journal of Biotechnology 215:44–51
Zurück zum Zitat Lee Y-C et al (2013) Lipid extractions from docosahexaenoic acid (DHA)-rich and oleaginous Chlorella sp. biomasses by organic-nanoclays. Bioresour Technol 137:74–81CrossRef Lee Y-C et al (2013) Lipid extractions from docosahexaenoic acid (DHA)-rich and oleaginous Chlorella sp. biomasses by organic-nanoclays. Bioresour Technol 137:74–81CrossRef
Zurück zum Zitat Mata TM, Santosa J, Mendesa AM, Caetanoa NS, Martinsc AA (2014) Sustainability Evaluation of Biodiesel Produced from Microalgae Chlamydomonas sp Grown in Brewery Wastewater Chemical. Engineering Transactions 37:823–828 Mata TM, Santosa J, Mendesa AM, Caetanoa NS, Martinsc AA (2014) Sustainability Evaluation of Biodiesel Produced from Microalgae Chlamydomonas sp Grown in Brewery Wastewater Chemical. Engineering Transactions 37:823–828
Zurück zum Zitat Okuda T, Ando A, Sakuradani E, Ogawa J (2013) Isolation and characterization of a docosahexaenoic acid-phospholipids producing microorganism Crypthecodinium sp. D31. J Am Oil Chem Soc 90:1837–1844CrossRef Okuda T, Ando A, Sakuradani E, Ogawa J (2013) Isolation and characterization of a docosahexaenoic acid-phospholipids producing microorganism Crypthecodinium sp. D31. J Am Oil Chem Soc 90:1837–1844CrossRef
Zurück zum Zitat Pulz O (2001) Photobioreactors: production systems for phototrophic microorganisms. Appl Microbiol Biotechnol 57:287–293CrossRef Pulz O (2001) Photobioreactors: production systems for phototrophic microorganisms. Appl Microbiol Biotechnol 57:287–293CrossRef
Zurück zum Zitat Pyle DJ, Garcia RA, Wen Z (2008) Producing docosahexaenoic acid (DHA)-rich algae from biodiesel-derived crude glycerol: effects of impurities on DHA production and algal biomass composition. J Agric Food Chem 56:3933–3939CrossRef Pyle DJ, Garcia RA, Wen Z (2008) Producing docosahexaenoic acid (DHA)-rich algae from biodiesel-derived crude glycerol: effects of impurities on DHA production and algal biomass composition. J Agric Food Chem 56:3933–3939CrossRef
Zurück zum Zitat Raheem A, Sivasangar S (2015) Wan Azlina WAKG, Taufiq Yap YH, Danquah MK, Harun R. Thermogravimetric study of Chlorella vulgaris for syngas production Algal Research 12:52–59. doi:10.1016/j.algal.2015.08.003 Raheem A, Sivasangar S (2015) Wan Azlina WAKG, Taufiq Yap YH, Danquah MK, Harun R. Thermogravimetric study of Chlorella vulgaris for syngas production Algal Research 12:52–59. doi:10.​1016/​j.​algal.​2015.​08.​003
Zurück zum Zitat Taoka Y, Nagano N, Okita Y, Izumida H, Sugimoto S, Hayashi M (2011) Effect of Tween 80 on the growth, lipid accumulation and fatty acid composition of Thraustochytrium aureum ATCC 34304. J Biosci Bioeng 111:420–424CrossRef Taoka Y, Nagano N, Okita Y, Izumida H, Sugimoto S, Hayashi M (2011) Effect of Tween 80 on the growth, lipid accumulation and fatty acid composition of Thraustochytrium aureum ATCC 34304. J Biosci Bioeng 111:420–424CrossRef
Zurück zum Zitat Ubando AT, Cuello JL, El-Halwagi MM, Culaba AB, Promentilla MAB, Tan RR (2015) Application of stochastic analytic hierarchy process for evaluating algal cultivation systems for sustainable biofuel production Clean Technologies and Environmental Policy:1-14 doi:10.1007/s10098-015-1073-z Ubando AT, Cuello JL, El-Halwagi MM, Culaba AB, Promentilla MAB, Tan RR (2015) Application of stochastic analytic hierarchy process for evaluating algal cultivation systems for sustainable biofuel production Clean Technologies and Environmental Policy:1-14 doi:10.​1007/​s10098-015-1073-z
Zurück zum Zitat Wan M et al (2011) The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella sorokiniana. Appl Microbiol Biotechnol 91:835–844CrossRef Wan M et al (2011) The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella sorokiniana. Appl Microbiol Biotechnol 91:835–844CrossRef
Zurück zum Zitat Xue J, Grift TE, Hansen AC (2011) Effect of biodiesel on engine performances and emissions. Renew Sustain Energy Rev 15:1098–1116CrossRef Xue J, Grift TE, Hansen AC (2011) Effect of biodiesel on engine performances and emissions. Renew Sustain Energy Rev 15:1098–1116CrossRef
Zurück zum Zitat Zhu L, Zhang X, Ji L, Song X, Kuang C (2007) Changes of lipid content and fatty acid composition of Schizochytrium limacinum in response to different temperatures and salinities. Process Biochem 42:210–214CrossRef Zhu L, Zhang X, Ji L, Song X, Kuang C (2007) Changes of lipid content and fatty acid composition of Schizochytrium limacinum in response to different temperatures and salinities. Process Biochem 42:210–214CrossRef
Metadaten
Titel
Docosahexaenoic acid production from crude glycerol by Schizochytrium limacinum SR21
verfasst von
Yun-Tsun Lung
Chung Hong Tan
Pau Loke Show
Tau Chuan Ling
John Chi-Wei Lan
Hon Loong Lam
Jo-Shu Chang
Publikationsdatum
19.02.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
Clean Technologies and Environmental Policy / Ausgabe 7/2016
Print ISSN: 1618-954X
Elektronische ISSN: 1618-9558
DOI
https://doi.org/10.1007/s10098-016-1126-y

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