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Journal of Nanoparticle Research
Erschienen in: Journal of Nanoparticle Research 5/2020

01.05.2020 | Research paper

Effect of Ag2S-BSA nanoparticle size on 3T3 fibroblast cell line cytotoxicity

verfasst von: Nataly Arrieta-Sandoval, Porfirio Estrada Rojas, Imelda Olivas-Armendáriz, Laura E. Valencia Gómez, Juan F. Hernández Paz, Blanca E. Monarrez Cordero, Claudia A. Rodríguez González

Erschienen in: Journal of Nanoparticle Research | Ausgabe 5/2020

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Abstract

Silver sulfide nanoparticles, smaller than 5 nm, are of great interest in the biomedical field due to their improved optical properties from the quantum confinement of charge carriers. The effect of silver sulfide nanoparticles (6.4 ± 2.2, 13 ± 4.9, and 33 ± 12.5 nm) coated with BSA (bovine serum albumin) on cytotoxicity, cell proliferation (MTT assay), and cell morphology was determined in vitro using 3T3 fibroblast cell line as assay model. The synthesized Ag2S-BSA nanoparticles were characterized by X-ray diffraction, infrared spectroscopy, and thermogravimetric analysis. Transmission electron microscopy and dynamic light scattering were used for particle size measurement. The MTT results showed a tendency to decrease cell viability with time exposure rather than concentration, in all particle’s groups. A greater affectation was observed in cells incubated with smaller nanoparticles, and this is associated with a higher speed of cell uptake due to a higher content of adsorbed BSA. It was found that the largest nanoparticles (33 ± 12.5 nm) at 72 h and at higher concentration presents the same cell viability as observed with the smallest attributing it to the agglomeration of particles formed by the low stability of the sample. Regarding cell morphology, fluorescence microscopy showed good cell growth in all the nanoparticle groups, observing a slighter increase in the cell population of the sample of intermediate particle size (13 ± 4.9 nm).
Vorheriger Artikel Development of a nanoparticle-based gradient method for simple and fast quantification of bacteria-nanoparticle conjugates
Nächster Artikel Targeted delivery of a short antimicrobial peptide against CD44-overexpressing tumor cells using hyaluronic acid-coated chitosan nanoparticles: An in vitro study

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Metadaten
Titel
Effect of Ag2S-BSA nanoparticle size on 3T3 fibroblast cell line cytotoxicity
verfasst von
Nataly Arrieta-Sandoval
Porfirio Estrada Rojas
Imelda Olivas-Armendáriz
Laura E. Valencia Gómez
Juan F. Hernández Paz
Blanca E. Monarrez Cordero
Claudia A. Rodríguez González
Publikationsdatum
01.05.2020
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 5/2020
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-020-04834-6

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