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Journal of Nanoparticle Research

Erschienen in:

01.03.2013 | Research Paper

Effect of DOPE and cholesterol on the protein adsorption onto lipid nanoparticles

verfasst von: Giulio Caracciolo, Daniela Pozzi, Anna Laura Capriotti, Chiara Cavaliere, Aldo Laganà

Erschienen in: Journal of Nanoparticle Research | Ausgabe 3/2013

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Abstract

Upon administration, nanoparticles (NPs) are exposed to biological fluids from which they adsorb proteins and other biomolecules to form a “protein corona”. NP–protein interactions are still poorly understood and quantitative studies to characterize them remain scarce. Here, we have investigated the effect of neutral dioleoylphosphatidylethanolamine (DOPE) and cholesterol on the adsorption of human plasma proteins onto the surface of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)-based cationic liposomes of 100 nm in diameter. Quantitative analysis of the protein corona revealed that replacing cationic DOTAP lipids with neutral lipids, being indifferently DOPE or cholesterol, reduces the affinity of fibrinogen, prothrombin, vitamin K, and vitronectin for the lipid surface. On the other side, DOPE specifically promotes the adsorption of apolipoproteins and serum albumin, while cholesterol induces the preferential binding of immunoglobulins and complement proteins. The results of this study will help to explain why NPs of different lipid compositions have a dramatic difference in their in vivo transfection efficiency and will be useful for design of lipid NPs with optimal circulation profiles.

Vorheriger Artikel Decoration of cesium iodide nano particles on patterned carbon nanotube emitter arrays to improve their field emission

Nächster Artikel A simple room-temperature synthesis of mesoporous silica rods with tunable size and porosity

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Titel: Effect of DOPE and cholesterol on the protein adsorption onto lipid nanoparticles
verfasst von: Giulio Caracciolo
Daniela Pozzi
Anna Laura Capriotti
Chiara Cavaliere
Aldo Laganà
Publikationsdatum: 01.03.2013
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 3/2013
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1498-4

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