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2011 | OriginalPaper | Buchkapitel

Effects of l-Proline and Cold Treatment on Pepper (Capsicum annuum L.) Anther Culture

verfasst von : D. Özkum, R. Tipirdamaz

Erschienen in: Survival and Sustainability

Verlag: Springer Berlin Heidelberg

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Abstract

Comparison of cold-shock treatments (at 4°C for 48 and 96 h) of pepper buds with a control (not subjected to cold treatments) has no effect on the production of embryo from cultured pepper anthers. The effects of cold shock treatments were not changed when the l-proline was added to the induction medium (40, 125 and 500 mg L−1). Cold treated anthers showed a lower response or non response than control anthers. Regeneration frequency did not appear to be affected by the presence of l-proline in the induction medium. The highest numbers of embryos (12.5 embryos/100 anthers) were obtained from the control anthers that were cultured on induction medium, MS medium, containing 4 mg L−1 naphthalene acetic acid (NAA) and 1 mg L−1 benzyladenine (BA) and activated charcoal without l-proline.

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Metadaten
Titel
Effects of l-Proline and Cold Treatment on Pepper (Capsicum annuum L.) Anther Culture
verfasst von
D. Özkum
R. Tipirdamaz
Copyright-Jahr
2011
Verlag
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/978-3-540-95991-5_14