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Journal of Polymer Research
Erschienen in: Journal of Polymer Research 3/2018

01.03.2018 | ORIGINAL PAPER

Effects of various monomers and micro-structure of polyhydroxyalkanoates on the behavior of endothelial progenitor cells and endothelial cells for vascular tissue engineering

verfasst von: Chao-Ling Yao, Jian-Haw Chen, Cheng-Hung Lee

Erschienen in: Journal of Polymer Research | Ausgabe 3/2018

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Abstract

Cardiovascular diseases are a leading cause of mortality in the world today. Vascular tissue engineering is an important and attractive research issue for the repair and regeneration of blood vessels. Two bio-based polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), which both belong to the polyhydroxyalkanoate (PHA) family, were used in this study. The aim of this study is to assess the potential application of PHB and PHBV to serve as a scaffold that is seeded with human umbilical vein endothelial cells (HUVECs) or endothelial progenitor cells (EPCs) for vascular tissue engineering. PHA films with various surface characteristics were prepared by solution-casting (surface roughness) and electrospinning (mesh-like structure). First, the mechanical and physical properties of various types of PHA films were analyzed. Then, the PHAs films were examined for cytotoxicity, biocompatibility and proliferation ability using cell lines (3 T3 and L929) and primary cells (HUVECs and EPCs). The cell morphology cultured on the PHA films was observed by fluorescence microscope and scanning electron microscopy. In addition, cultured EPCs on various types of PHA films were analyzed for whether the cells maintained the abilities of Ac-LDL uptake and UEA-1 lectin binding and exhibited specific gene expressions, including VEGFR-2, vWF, CD31, CD34 and CD133. Importantly, the cell retention rate and anti-coagulation ability of HUVECs or EPCs cultured on the various types of PHA films were also evaluated at the indicated time points. Our results showed that PHA films that were prepared using electrospinning methods (Ele-PHB and Ele-PHBV) had good mechanical and physical properties. HUVECs and EPCs can attach and grow on Ele-PHB and Ele-PHBV films without showing cytotoxicity. After a one-week culture, expanded HUVECs or EPCs maintained the correct cell morphologies and exhibited correct cell functions, such as high cell attachment rate and anti-coagulation ability. Taken together, Ele-PHB and Ele-PHBV films were ideal bio-based polymers to combine with HUVECs or EPCs for vascular tissue engineering.
Vorheriger Artikel Synthesis, characterization and photo – switching properties of azobenzene mesogen containing poly (ether - ester) s from cashew nut shell liquid
Nächster Artikel Influence of the hydroquinone ether moieties and Bisphenol A glycerolate diacrylate on the UV stability behavior of new polyurethane materials

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Metadaten
Titel
Effects of various monomers and micro-structure of polyhydroxyalkanoates on the behavior of endothelial progenitor cells and endothelial cells for vascular tissue engineering
verfasst von
Chao-Ling Yao
Jian-Haw Chen
Cheng-Hung Lee
Publikationsdatum
01.03.2018
Verlag
Springer Netherlands
Erschienen in
Journal of Polymer Research / Ausgabe 3/2018
Print ISSN: 1022-9760
Elektronische ISSN: 1572-8935
DOI
https://doi.org/10.1007/s10965-017-1341-1

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