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2013 | OriginalPaper | Buchkapitel

Hairy Roots as a Vaccine Production and Delivery System

verfasst von : Marina Skarjinskaia, Karen Ruby, Adriana Araujo, Karina Taylor, Vengadesan Gopalasamy-Raju, Konstantin Musiychuk, Jessica A. Chichester, Gene A. Palmer, Patricia de la Rosa, Vadim Mett, Natalia Ugulava, Stephen J. Streatfield, Vidadi Yusibov

Erschienen in: Biotechnology of Hairy Root Systems

Verlag: Springer Berlin Heidelberg

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Abstract

Prevention of infectious diseases by vaccination is often limited because of the lack of safe, effective, and accessible vaccines. Traditional vaccines are expensive and require special conditions for storage, distribution, and administration. Plants have potential for large-scale production of a variety of inexpensive and highly effective recombinant proteins for biomedical and pharmaceutical applications, including subunit vaccines. There are several approaches for the production of vaccine antigens in plants, including transient expression systems based on Agrobacterium delivery of binary vectors or plant viral vectors, stable transgenic plants, and plant cell or tissue cultures. Axenic plant cultures maintained under defined physical and chemical conditions appear to be an attractive production platform when target proteins need to be synthesized in a fully controlled environment. Hairy root cultures meet the criteria for such a system. Hairy root cultures, generated from edible plants and producing target antigens, provide a potential approach for the development of vaccines for oral delivery. With this approach, there are no protein extraction and purification costs and the active biomolecule is protected by the plant cell wall during passage through the upper gastrointestinal tract. This allows for gradual release of antigen at mucosal surfaces in the gut. Lyophilized hairy root cultures expressing vaccine antigens can be stored at ambient temperature for extended periods of time, which should facilitate storage and distribution, ultimately allowing for large populations to be vaccinated.

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Vorheriges Kapitel Hairy Root Culture: Bioreactor Design and Process Intensification

Nächstes Kapitel Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures

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Titel: Hairy Roots as a Vaccine Production and Delivery System
verfasst von: Marina Skarjinskaia
Karen Ruby
Adriana Araujo
Karina Taylor
Vengadesan Gopalasamy-Raju
Konstantin Musiychuk
Jessica A. Chichester
Gene A. Palmer
Patricia de la Rosa
Vadim Mett
Natalia Ugulava
Stephen J. Streatfield
Vidadi Yusibov
Verlag: Springer Berlin Heidelberg
Buch: Biotechnology of Hairy Root Systems
Print ISBN: 978-3-642-39018-0

Electronic ISBN: 978-3-642-39019-7

Copyright-Jahr: 2013
DOI: https://doi.org/10.1007/10_2013_184

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