2011 | OriginalPaper | Buchkapitel
Image Analysis for Calculation of the Toxicity Degree of Cells in Phase Contrast Microscopy Images
verfasst von : M. Athelogou, M. Eblenkamp, G. Schmidt, F. Novotny, E. Wintermantel, G. Binnig
Erschienen in: Bildverarbeitung für die Medizin 2011
Verlag: Springer Berlin Heidelberg
Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.
Wählen Sie Textabschnitte aus um mit Künstlicher Intelligenz passenden Patente zu finden. powered by
Markieren Sie Textabschnitte, um KI-gestützt weitere passende Inhalte zu finden. powered by
Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.