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Journal of Nanoparticle Research

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01.01.2013 | Research Paper

Bone regeneration based on nano-hydroxyapatite and hydroxyapatite/chitosan nanocomposites: an in vitro and in vivo comparative study

verfasst von: S. Tavakol, M. R. Nikpour, A. Amani, M. Soltani, S. M. Rabiee, S. M. Rezayat, P. Chen, M. Jahanshahi

Erschienen in: Journal of Nanoparticle Research | Ausgabe 1/2013

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Abstract

Surface morphology, surface wettability, and size distribution of biomaterials affect their in vitro and in vivo bone regeneration potential. Since nano-hydroxyapatite has a great chemical and structural similarity to natural bone and dental tissues, incorporated biomaterial of such products could improve bioactivity and bone bonding ability. In this research, nano-hydroxyapatite (23 ± 0.09 nm) and its composites with variety of chitosan content [2, 4, and 6 g (45 ± 0.19, 32 ± 0.12, and 28 ± 0.12 nm, respectively)] were prepared via an in situ hybridization route. Size distribution of the particles, protein adsorption, and calcium deposition of powders by the osteoblast cells, gene expression and percentage of new bone formation area were investigated. The highest degree of bone regeneration potential was observed in nano-hydroxyapatite powder, while the bone regeneration was lowest in nano-hydroxyapatite with 6 g of chitosan. Regarding these data, suitable size distribution next to size distribution of hydroxyapatite in bone, smaller size, higher wettability, lower surface roughness of the nano-hydroxyapatite particles and homogeneity in surface resulted in higher protein adsorption, cell differentiation and percentage of bone formation area. Results obtained from in vivo and in vitro tests confirmed the role of surface morphology, surface wettability, mean size and size distribution of biomaterial besides surface chemistry as a temporary bone substitute.

Vorheriger Artikel Stable aqueous dispersion of superparamagnetic iron oxide nanoparticles protected by charged chitosan derivatives

Nächster Artikel Human serum albumin as protecting agent of silver nanoparticles: role of the protein conformation and amine groups in the nanoparticle stabilization

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Ahmed R, Faisal N, Paradowska A, Fitzpatrick M, Khor K (2011) Neutron diffraction residual strain measurements in nanostructured hydroxyapatite coatings for orthopaedic implants. J Mech Behav Biomed Mater 4(8):2043–2054CrossRef

Ao Q, Fung C, Tsui A, Cai S, Zuo H, Chan Y, Shum D (2011) The regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells. Biomaterials 32(3):787–796CrossRef

Att W, Hori N, Iwasa F, Yamada M, Ueno T, Ogawa T (2009a) The effect of UV-photofunctionalization on the timerelated bioactivity of titanium and chromium-cobalt alloys. Biomaterials 30(26):4268–4276CrossRef

Att W, Hori N, Takeuchi M, Ouyang J, Yang Y, Anpo M, Ogawa T (2009b) Time-dependent degradation of titanium osteoconductivity: an implication of biological aging of implant materials. Biomaterials 30(29):5352–5363CrossRef

Bin L, Longnan H, Xinbo W, Jinhuan M, Fang X (2011) Biodegradation and compressive strength of phosphorylated chitosan/chitosan/hydroxyapatite bio-composites. Mater Des 32(8–9):4543–4547

Chen J, Nan K, Yin S, Wang Y, Wu T, Zhang Q (2010) Characterization and biocompatibility of nanohybrid scaffold prepared via in situ crystallization of hydroxyapatite in chitosan matrix. Colloids Surf B 81(2):640–647CrossRef

Chen Y, Huang Z, Li X, Li S, Zhou Z, Zhang Y, Feng Q, Yu B (2012a) In vitro biocompatibility and osteoblast differentiation of an injectable chitosan/nano-hydroxyapatite/collagen scaffold. J Nanomater 1–6:Article ID 401084

Chen Y, Li S, Li X, Zhang Y, Huang Z, Feng Q, Zhou Z, Lin B, Yu B (2012b) Noninvasive evaluation of injectable chitosan/nano-hydroxyapatite/collagen scaffold via ultrasound. J Nanomater 1–7:Article ID 939821

Chesnutt BM, Yuan Y, Buddington K, Haggard WO, Bumgardner JD (2009) Composite chitosan/nano-hydroxyapatite scaffolds induce osteocalcin production by osteoblasts in vitro and support bone formation in vivo. Tissue Eng Part A 15(9):2571–2579CrossRef

Clarke SA, Hoskins NL, Jordan GR, Henderson SA, Marsh DR (2007) In vitro testing of advanced JAX bone void filler system: species differences in the response of bone marrow stromal cells to β tri-calcium phosphate and carboxymethylcellulose gel. J Mater Sci Mater Med 18(12):2283–2290CrossRef

Dalby M, David M, Mary R, Hossein A, Duncan S, Stanley A, Richard O (2006) Osteoprogenitor response to semi-ordered and random nanotopographies. Biomaterials 27(15):2980–2987CrossRef

Fang L, Leng Y, Gao P (2005) Processing of hydroxyapatite reinforced ultrahigh molecular weight polyethylene for biomedical applications. Biomaterials 26(17):3471–3478CrossRef

Hassenkam T, Fantner G, Cutroni J, Weaver J, Morse D, Hansma P (2004) High-resolution AFM imaging of intact and fractured trabecular bone. Bone 35(1):4–10CrossRef

Huang Z, Yu B, Feng Q, Li S, Chen Y, Luo L (2011) In situ-forming chitosan/nano-hydroxyapatite/collagen gel for the delivery of bone marrow mesenchymal stem cells. Carbohydr Polym 85(1):261–267CrossRef

Kim K, Dean D, Lu A, Mikos A, Fisher J (2011) Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in biodegradable nanocomposite scaffolds. Acta Biomater 7(3):1249–1264CrossRef

Kong L, Gao Y, Cao W, Gong Y, Zhao N, Zhang X (2005) Preparation and characterization of nano-hydroxyapatite/chitosan composite scaffolds. J Biomed Mater Res Part A 75(2):275–282CrossRef

Kong L, Gao Y, Lu G, Gong Y, Zhang X (2006) A study on the bioactivity of chitosan/nano-hydroxyapatitecomposite scaffolds for bone tissue engineering. Eur Polym J 42:3171–3179CrossRef

Kumar M, Muzzarelli R, Muzzarelli C, Sashiwa H, Domb A (2004) Chitosan chemistry and pharmaceutical perspectives. Chem Rev 104(12):6017–6084CrossRef

Luo X, Zhang L, Morsi Y, Zou Q, Wang Y, Gao S, Li Y (2011) Hydroxyapatite/polyamide 66 porous scaffold with an ethylene vinyl acetate surface layer used for simultaneous substitute and repair of articular cartilage and underlying bone. Appl Surf Sci 257(23):9888–9894CrossRef

Manjubala I, Scheler S, Bossert J, Jandt K (2006) Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique. Acta Biomater 2(1):75–78CrossRef

Mohamed K, M.El-Rashidy Z, Salamai A (2011) In-vitro properties of nano-hydroxyapatite/chitosan biocomposites. Ceram Int 37:3265–3271CrossRef

Muzzarelli R (2011) Chitosan composites with inorganics, morphogenetic proteins and stem cells, for bone regeneration. Carbohydr Polym 83(4):1433–1445CrossRef

Notodihardjo FZ, Kakudo N, Kushida S, Suzuki K, Kusumoto K (2012) Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats. J Craniomaxillofac Surg 40(3):287–291CrossRef

Qiaoling H, Baoqiang L, Mang W, Jiacong S (2004) Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: a potential material as internal fixation of bone fracture. Biomaterials 25(5):779–785CrossRef

Roy M, Bandyopadhyay A, Bose S (2011) Induction plasma sprayed nano hydroxyapatite coatings on titanium for orthopaedic and dental implants. Surf Coat Technol 205(8–9):2785–2792CrossRef

Rusu V, Ng C, Wilke M, Tiersch B, Fratzl P, Peter M (2005) Size controlled hydroxyapatite nanoparticles as self-organized organic–inorganic composite materials. Biomaterials 26(26):5414–5426CrossRef

San Thian E, Huang J, Barber Z, Best S, Bonfield W (2011) Surface modification of magnetron-sputtered hydroxyapatite thin films via silicon substitution for orthopaedic and dental applications. Surf Coat Technol 205(11):3472–3477CrossRef

Saravanan S, Nethala S, Pattnaik S, Tripathi A, Moorthi A, Selvamurugan N (2011) Preparation, characterization and antimicrobial activity of a bio-composite scaffold containing chitosan/nano-hydroxyapatite/nano-silver for bone tissue engineering. Int J Biol Macromol 49(2):188–193CrossRef

Silva G, Czeisler C, Niece K, Beniash E, Harrington D, Kessler J et al (2004) Selective differentiation of neural progenitor cells by high-epitope density nanofibers. Science 303:1352–1355CrossRef

Tan F, Naciri M, Dowling D, Al-Rubeai M (2012) In-vitro and in-vivo bioactivity of CoBlast hydroxyapatite coating and the effect of impaction on its osteoconductivity. Biotechnol Adv 30(1):352–362CrossRef

Tavakol S, Ragerdi Kashani I, Azami M, Khoshzaban A, Tavakol B, Kharrazi S, Ebrahimi S, Rezayat Sorkhabadi SM (2012) In vitro and in vivo investigations on bone regeneration potential of laminated hydroxyapatite/gelatin nanocomposite scaffold along with DBM. J Nanopart Res 14:1265CrossRef

Teng S-H, Lee E-J, Yoon B-H, Shin D-S, Kim H-E, Oh J-S (2009) Chitosan/nanohydroxyapatite composite membranes via dynamic filtration for guided bone regeneration. J Biomed Mater Res A 88(3):569–580

Wei J, Igarashi T, Okumori N, Igarashi T, Maetani T, Liu B, Yoshinari M (2009) Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts. Biomed Mater 4(4):045002CrossRef

Xu H, Carl S (2005) Fast setting calcium phosphate–chitosan scaffold: mechanical properties and biocompatibility. Biomaterials 26(12):1337–1348CrossRef

Yim E, Pang S, Leong K (2007) Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. Exp Cell Res 313(9):1820–1829CrossRef

Zhang L, Ao Q, Wang A, Lu G, Kong L, Gong Y (2006) A sandwich tubular scaffold derived from chitosan for blood vessel tissue engineering. J Biomed Mater Res Part A 77(2):277–284CrossRef

Zhao F, Grayson W, Ma T, Bunnell B, Lu W (2006) Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell constructs development. Biomaterials 27(9):1859–1867CrossRef

Zhao Y, Zhang Y, Ning F, Guo D, Xu Z (2007) Synthesis and cellular biocompatibility of two kinds of HAP with different nanocrystal morphology. J Biomed Mater Res Part B: Appl Biomater 83(1):121–126CrossRef

Zhou H, Lee J (2011) Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater 7(7):2769–2781CrossRef

Titel: Bone regeneration based on nano-hydroxyapatite and hydroxyapatite/chitosan nanocomposites: an in vitro and in vivo comparative study
verfasst von: S. Tavakol
M. R. Nikpour
A. Amani
M. Soltani
S. M. Rabiee
S. M. Rezayat
P. Chen
M. Jahanshahi
Publikationsdatum: 01.01.2013
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 1/2013
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-012-1373-8

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