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Journal of Nanoparticle Research

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01.08.2013 | Research Paper

Polyinosinic:polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles

verfasst von: Rouhollah Khodadust, Pelin Mutlu, Serap Yalcın, Gozde Unsoy, Ufuk Gunduz

Erschienen in: Journal of Nanoparticle Research | Ausgabe 8/2013

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Abstract

Poly (I:C), which is a synthetic double-stranded RNA, have significant toxicity on tumor cells. The immobilization of Poly (I:C) onto nanoparticles is important for the fabrication of targeted delivery systems. In this study, different generations of newly synthesized PAMAM dendron-coated magnetic nanoparticles (DcMNP) which can be targeted to the tumor site under magnetic field were efficiently loaded for the first time with Poly (I:C). Different generations of DcMNPs (G₂, G₃, G₄, G₅, G₆, and G₇) were synthesized. Poly (I:C) activation was achieved in the presence of EDC and 1-methylimidazole. Loading of Poly (I:C) onto DcMNPs was followed by agarose gel electrophoresis. Acidic reaction conditions were found as superior to basic and neutral for binding of Poly (I:C). In addition, having more functional groups at the surface, higher generations (G₇, G₆, and G₅) of PAMAM DcMNPs were found more suitable as a delivery system for Poly (I:C). Further in vitro and in vivo analyses of Poly (I:C)/PAMAM magnetic nanoparticles may provide new opportunities for the selective targeting and killing of tumor cells.

Vorheriger Artikel Comparison between stability, electronic and structural properties of noble metal nanoclusters

Nächster Artikel Single-wall carbon nanohorns (SWNHs) inhibited proliferation of human glioma cells and promoted its apoptosis

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Titel: Polyinosinic:polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles
verfasst von: Rouhollah Khodadust
Pelin Mutlu
Serap Yalcın
Gozde Unsoy
Ufuk Gunduz
Publikationsdatum: 01.08.2013
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 8/2013
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1860-6

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