²²²Rn and CO₂ monitoring in soil and indoor atmosphere to understand changes in the gaseous dynamics of Rull cave (Spain)

The study site is located in Rull cave (38° 48′ 40″ N, 0° 10′ 38″ W, Vall d’Ebo, Alicante), in Southeast Spain. Nearly 10 years of data sets (2012–2022) were produced from the continuous measurements of environmental variables and gaseous concentrations within the cave and the soil above it.

Above the cave, the silty-silty loam soil profile has a thickness below 1 m and presents a fine to coarse texture with no differentiated horizons. Soil is mainly composed of clay minerals and fine quartz, calcite and feldspars grains. Rull cave is developed in both massive Miocene conglomerates with considerable textural and petrophysical complexity, which were deposited on Cretaceous limestones (Pla et al. 2016a, 2017). These conglomerates conform the host rock of the cave, which has a thickness varying from 9 to 23 m. Conglomerates are composed of limeclasts, calcite cement, and matrix, which has a calcitic composition with a minor amount of iron oxides, quartz grains and clay minerals.

Inside the cave, calcite speleothems such as stalactites, columns, flowstones and draperies are common, and fallen blocks of different sizes are present due to old ceiling collapses (or breakdown) (Pla et al. 2014). Cave sediments came from weathering detritus (the insoluble component of the bedrock, left behind when the bedrock is dissolved) and soil washdown (sediments that migrate into the cave from the land surface above it). Cave sediments are distributed throughout the cave floor, which contain calcite, quartz, clay minerals, and feldspars. The uranium and thorium composition (obtained from ICP-MS; ALS Limited) present different values according to their mineralogical composition and clay content. The uranium concentrations for the soil, host-rock, cave sediments, and speleothems are, respectively, 3.14, 0.37, 2.23 and 0.09 ppm, whereas thorium concentrations are, respectively, 14.35, 0.09, 10.4 and < 0.05 ppm.

The cave is a roughly rounded single room with an exposed surface of 1535 m² and holding 9915 m³ inside, with the entrance located in the highest sector. The height inside the room is variable, with a maximum of 20 m (Fig. 1). Multiple C3 plants, Mediterranean vegetation and microorganisms grow in this soil profile (Pla et al. 2016a, b, 2017). The site has a Csa climate type, according to a slightly modified Köppen–Geiger Classification (AEMET-IM 2011), which consists of warm annual temperatures with a dry and hot summer.

At the cave location and for the studied period (November 2012–July 2022), the environmental variables considered were temperature, relative humidity and pressure, with daily average values of 16.1 °C, 69.9% and 963.1 mbar, respectively. The average annual precipitation (2013–2021) is 553 mm. In the cave interior, temperature, relative humidity and pressure are, respectively, 16.2 °C, 97.6% and 963.3 mbar. Daily average CO₂ and ²²²Rn concentrations are 2008 ppm and 1745 Bq/m³, respectively. In addition, the cave annually receives, on average, 15,000 visitors although the number changes over the year affected by seasonality. Easter period and summer months present the highest affluence of people.

From February 2015 to July 2022 the conditions of the outside soil above the cave were also recorded. In this period the daily average soil temperature and volumetric water content were 16.3 °C and 0.21 m³/m³, respectively.

Environmental measurements in Rull Cave were performed with different weather stations and environmental probes, which changed within the study period (Pla et al. 2016a, 2017). Currently there is one datalogger HOBO H22-001 (Onset Computer, USA) recording synchronous and continuous microclimatic measurements inside the cave. It is connected to the electrical supply, and it has a security battery to ensure autonomy in case of power failure. The probes connected to the datalogger provide hourly temperature and relative humidity (HMP45AC, Vaisala, Finland; accuracies of ± 0.2 °C and ± 2.0%, respectively), CO₂ concentration (GMP252, Vaisala, Finland; accuracy of ± 40 ppm and measurement range of 0–10,000 ppm) and barometric pressure (S-BPB-CM50 Sensor, Onset Computer, USA; accuracy of ± 3.0 mbar). In addition, a Radim 5WP radon monitor (SSM&SISIE, Prague; accuracy of ± 12 Bq/m³) measures ²²²Rn concentration, also with hourly periodicity.

Atmospheric conditions outside the cave are continuously measured using a H21 Hobo Weather Station (Onset Computer, USA) coupled with an S-THB-M002 temperature and relative humidity sensor (Onset Computer, USA; accuracies of ± 0.21 °C and ± 2.5%, respectively), an S-BPB-CM50 barometric pressure sensor (Onset Computer, USA; accuracy of ± 3.0 mbar) and an S-RGF-M002 Davis rain gauge sensor (Onset Computer, USA; accuracy of ± 4%).

Hourly measurements of soil conditions (temperature and volumetric water content) are performed with a HOBO U12 logger (Onset, USA; accuracy of ± 0.5 °C) and an ECHO EC-5 probe (Decagon Devices, USA; accuracy of ± 1–2%) connected to an Em5b logger (Decagon Devices, USA). These probes are located at 10 cm depth in the soil cave.

For the 10-year period under analysis, some data are missing due to instrument failure or temporary power cuts affecting some probes. In the case of the CO₂ and ²²²Rn time series, the seasonal variations of both tracer gases are coeval, so the data gaps for CO₂ could be easily inferred from the time evolution of radon levels.

Since January 2014, discrete sampling of air from the outside atmosphere, soil air and cave atmosphere are performed to characterise the spatial distribution and temporal variation of CO₂ concentration and its δ¹³C signature. The sampling period varies through the study period. In the sampling procedure, air is pumped and saved into 1 L Tedlar gas sampling bags, and then analysed using a Picarro G2101-i analyser (California, USA; accuracy of ± 0.3‰ for δ¹³CO₂ after 5 min of analysis) which uses cavity ring-down spectroscopy (CRDS-WS) (Crosson 2008).

Three in-house standards with certified gas mixtures and known CO₂ concentration (7000 ppm, 400 ppm, and zero-CO₂), supplied by Abello Linde-Spain, were used at the beginning and the end of each analytical session by CRDS to calibrate the CO₂ concentration values from the air samples. The proper functioning of the CRDS analyser and its performance specifications regarding δ¹³C-CO₂ analyses were periodically checked and calibrated relative to two reference standards (USGS40 with δ¹³C VPDB =  − 26.4‰ and USGS41a with δ¹³C VPDB =  + 37.6‰, supplied by USGS/Reston Stable Isotope Laboratory), whose carbon isotopic signal was regularly determined by a combustion module (Costech, USA) coupled to the CRDS analyser (CM-CRDS system). The consecutive rounds of δ¹³C-CO₂ analyses of air samples were calibrated against the CO₂ obtained by CM-CRDS system for the following internal secondary standards: NaHCO₃ (δ¹³C VPDB =  − 10.9‰), sugarcane (δ¹³C VPDB =  − 11.7‰), acetanilide (δ¹³C VPDB =  − 26.3‰) and urea (δ¹³C VPDB =  − 49.2‰).

Since September 2021, discrete sampling of ²²²Rn concentration in soil gas and cave air is also monthly conducted. The gas inside the 2 L Tedlar sampling bags, collected in the cave and from the soil, is measured using AlphaGUARD DF2000 (Saphymo GmbH, Frankfurt am Main, Germany) which has a pulse ionization chamber (alpha spectroscopy) that allows measurement in diffusion and flow operation modes. For this study, the air samples were analysed using the 1-min flow mode, with a 0.3 L/min pump flow, over a 15-min period. A loop was employed for the analysis to measure the gas contained in different sampling bags collected in identical locations.

Radon exhalation, E (Bq/(m²s)), was estimated in an accumulation chamber of PMMA (polymethyl methacrylate), with 20 cm inner diameter. The chamber was located over a single PVC collar, which was permanently installed in the soil. The accumulation phase initially describes a linear growth with time, t (s), of the radon concentration in the accumulation chamber [²²²Rn] (Bq/m³). Radon exhalation (E) is calculated as (Eq. 1):where V (m³) is the effective accumulation chamber volume and S (m²) is the exhaling soil surface within the accumulation chamber. The ²²²Rn concentration was recorded using an AlphaE (Saphymo GmbH, Frankfurt am Main, Germany) for 4 h.

Since September 2021, inside the cave, the CO₂ and ²²²Rn discrete sampling was carried out while simultaneously measuring air temperature at each sampling point with a Vaisala HM70 portable probe (Vaisala, Finland; accuracy of ± 0.2 °C).

For the 10-year period under analysis (Fig. 2), Rull cave exhibits an annual gaseous cycle with two differentiated states. This pattern has also been found in many studies of gas dynamics within caves (Cao et al. 2021; Liñán et al. 2008; Wong and Banner 2010). In summer, with exterior temperatures (T_out) being higher than temperature inside the cave (T_in) (Fig. 2d), the cave atmosphere remains stagnated. This period is characterised by an increased accumulation of both gases CO₂ and ²²²Rn, reaching the maximum concentrations registered for the annual cycle. On the contrary, during the colder period of the year, the cave enters a ventilation stage with the air renewal causing a remarkable depletion of the gaseous concentrations (Fig. 2a, b). These seasonal variations in CO₂ and ²²²Rn concentrations are mainly a consequence of the relationships between external and cave temperatures (Pla et al. 2016a; Fig. 2a, b, d). The time series resulting of continuous measurements in Rull cave indicates that the average daily values of CO₂ and ²²²Rn concentrations vary from a minimum of 478 ppm and 404 Bq/m³, respectively (October to March/April), to a maximum of 3966 ppm and 4185 Bq/m³ (April/May to September). Although the cave receives visitors almost every day (Fig. 2b), CO₂ variations due to these visits are not substantial, and baseline concentrations are recovered after a short time period which varies, normally, from 1 to 72 h (Pla et al. 2016b, 2020). Seasonal variations of external temperature changed throughout the annual cycles and, consequently, the duration of each seasonal pattern of thermal gradient between the cave and the outer atmosphere (T_in > T_out or, on the contrary, T_in < T_out) varies from year to year. This fact implies that the beginning and end of each stage defined by the tracer gases concentrations and their variations (ventilation and stagnation) present inter-annual variations, although the amplitude of the seasonal variations of both tracer gases are similar over the years (Fig. 2a, b, d). This triggers the cave gaseous recharge or discharge, which is nevertheless also influenced by other environmental variables such as rainfall, soil temperature or soil water content (Fig. 2c) (Pla et al. 2016a, 2020). Atmospheric pressure and pressure inside the cave are nearly coincident, which points to the permanent barometric equilibrium between both environments (Fig. 2d).

Continuous measurements of temperature (T_in) inside Rull cave confirm that its atmosphere presents high environmental stability, with annual variations of ± 1.4 °C (Fig. 2d). For the 10-year studied period the maximum measured temperature is 16.8 °C (during winter), while the lowest was 15.4 °C (during summer). In addition, discrete measurements of cave temperature (turned into average values) performed from September 2021 to August 2022 (Fig. 3), indicate that the sector closest to the entrance is the warmest, especially the westernmost sector in which the overlying bedrock has the smallest thickness (Figs. 1, 3). The southernmost sector, farthest from the cave entrance, presents the lowest temperatures.

Spatial distribution of CO₂ and ²²²Rn (average values of the discrete sampling; September 2021–August 2022) reveals that in the two stages of the cave (stagnation and ventilation), the lowest concentrations of both gases are found near the entrance (Fig. 4) highlighting that this area presents the higher rate of air exchange with the exterior. The spatial distribution of both gases follows a similar pattern directly related to the morphology and the cave air circulation. Results obtained from the discrete sampling are coincident with the continuous measurements of gas concentration, pointing to a predominant air renewal in the coldest months when the concentration reaches the minimum values. During the stagnation period, the maximum values of CO₂ and ²²²Rn concentration in the cave atmosphere confirm that in the warmest months the air exchange rate with the external atmosphere is at the minimum.

Previous studies confirm that the Rull cave gaseous dynamics is governed by diffusive and advective fluxes responsible for the final gas concentrations in the cave atmosphere (Pla et al. 2016a, 2017). Both processes can exist simultaneously, and the prevalence of one over the other during a certain period is caused by the relationship between the different environmental variables in atmosphere, soil, and cave. When the cave temperature (T_in) is higher than the outdoor temperature (T_out) in the coldest months of the year, an advective gaseous flux between the exterior and interior air masses is predominant because of the density difference. On the contrary, when the relationship between temperatures is inverted, the ventilation due to the density gradient becomes non-existent and the colder and thus denser air within the cave remains nearly stagnant. During this stage, the gaseous diffusion from soil towards the cave contributes (depending on soil and cave conditions) to the increase in CO₂, reaching higher concentrations that last until the exterior temperature decreases below the cave temperature. Maximum CO₂ concentration in the cave is a consequence of the CO₂ soil production (Amundson et al. 1998; Faimon et al. 2012; Garcia-Anton et al. 2017) and its diffusion rates (Jabro et al. 2012; Jassal et al. 2004; Wang et al. 2019). Therefore, although CO₂ ranges from 3500 to 3900 ppm during summer, the precise annual maximum value is defined every year by soil respiration and a prevailing gaseous transport in the soil-rock porous system (Pla et al. 2016a). The incoming flux of soil-derived CO₂ to the cave atmosphere is noticed by the decrease of the isotopic signature δ¹³C-CO₂ as CO₂ of cave air rises.

The sampling of soil CO₂ above Rull cave has been carried out since 2014 (Fig. 5). Soil CO₂ also presents annual variations, although they are less recognizable than the cycles inside the cave, probably because of the discrete measurements not being performed with a regular time interval between them. Measurements in April–May–June and September–October usually present the highest values of soil CO₂ concentrations (Fig. 5) although this varies between cycles. The maximum CO₂ concentrations in soil, occurred during spring, are a consequence of the soil production under moderate soil temperatures and are influenced by the soil water content, thus related to rainfall. For instance, the rainfall occurred between March and May in 2022 (778 mm) was extraordinarily abundant when compared to the rest of the studied time series (the average annual precipitation is 553 mm for the period 2013–2021) and coincident with the maximum measured soil CO₂ concentration (4866 ppm) in May 2022. High soil CO₂ concentrations measured in autumn (October 2014: 2622 ppm; October 2017: 2714 ppm; September 2019: 4841 ppm) are also related to moderate soil temperatures and enough water within the soil due to rainfall. Maximum soil CO₂ values registered in spring normally coincide with lower CO₂ cave concentrations (Fig. 5) and maximum soil CO₂ values registered in autumn normally coincide with decreasing CO₂ cave concentrations.

Relationships between soil-cave-atmosphere are highlighted with the results of the air sampling and the analyses of the CO₂ abundance and its carbon isotopic signature. Within the soil, carbon dioxide is mainly derived from the respiration of living plants and the decomposition of organic matter by soil microorganisms (Amundson et al. 1998). CO₂ derived from C3 plant roots and microbial respiration in soils with organic matter content is characterised by values of δ¹³C-CO₂ ranging from − 25.0 to − 27.5‰ VDP (Amundson et al. 1998; Cerling et al. 1991; Di Martino et al. 2016, 2020; Kuzyakov 2006). Consequently δ¹³C-CO₂ evolution in soil and inside Rull cave emphasises the periods of major soil CO₂ production (lowest values of δ¹³C-CO₂ in soil being coincident with maximum soil CO₂ concentration). The soil-produced CO₂ can diffuse into the cave atmosphere under certain conditions of soil porous network. Heavy isotopes diffuse more slowly, leading to an enrichment of ¹²C (lighter δ¹³C-CO₂ values) of the soil-derived CO₂. A maximum theoretical kinetic fractionation of 4.4‰ has been reported for the diffusion processes through the soil (Capasso et al. 2001). This transport requires a certain time, but when this diffused soil CO₂ enters the cave, it causes a likely ¹³C depletion of CO₂ reaching the lowest values (lightest). There is a delay between maximums in soil and cave because of the gaseous transport through soil and rock.

When soil CO₂ mixes with the cave gaseous atmosphere it is diluted with the external air (enriched in δ¹³C-CO₂) that enters to Rull cave by advective mechanisms predominant from October to April–May (Fig. 5) and which is responsible for the lowest CO₂ concentrations in the cave atmosphere.

The Keeling-plot approach (Keeling 1958) is based on a simplified two-end member model where the concentration and isotopic ratio of the cave-air CO₂ result from the proportional mixing of the background atmosphere and a second source (soil-derived) with an isotopically light CO₂-rich component. Two results can be obtained from the Keeling plot: (1) the intercept value of the lineal Keeling function in the δ¹³C-CO₂ axis corresponds to the theoretical isotopic signal of the soil-derived CO₂ source, and (2) the relative contribution of CO₂ from the outer atmosphere that is present in the cave air, in function of the proximity of the data pairs of cave air to each end member. The Keeling plot for the atmosphere-soil-cave system demonstrates that the CO₂ concentration in Rull cave air is the result of mixing the background atmospheric CO₂ with the soil-produced one. This diagram highlights the prevalence of the soil organic CO₂ component in the cave atmosphere. The linear fitting of the data, obtained from the discrete samplings, intercepts the y-axis ranging from − 24.80 to − 26.55‰ (Fig. 6), which is in the range defining the characteristic values for δ¹³C-CO₂ derived from C3 plant roots and microbial respiration (Amundson et al. 1998; Cerling et al. 1991; Kuzyakov 2006).

The y-axis interception value for both stages confirms that CO₂ within the cave primarily comes from roots and microbial respiration in the soil above the cave. The most δ¹³C-depleted air CO₂ during the ventilation stage highlights the predominance of the soil CO₂ origin also when rates of dilution are predominant in the cave atmosphere. In the periods with higher external temperatures and scarce rainfall, the porous network of soil keeps open and connected to the external atmosphere. As a consequence, the direct connection between soil and cave is neglected because soil produced CO₂ migrates to the external atmosphere. On the contrary, with moderate temperatures and water content in soil (on average, nearly 15 °C and 0.17), diffusion from soil to cave is active and coincident with ventilation (early spring). This triggers that in the ventilation stage (Fig. 6a) the y-intercept value is more negative (closer to the soil δ¹³C-CO₂ value) than in the stagnation period. During the ventilation stage, the porous network of soil is partially filled with water and a CO₂ influx from soil to cave atmosphere prevails in comparison with gas fluxes from soil to the open atmosphere. This provokes an input of ¹²C-depleted gas (soil-derived CO₂) to the cave at atmosphere and, consequently, the y-intercept value of the Keeling plot becomes more negative during this stage.

Although ²²²Rn production mechanism is different from CO₂, the ²²²Rn concentration within the cave describes the same annual cycles than CO₂ (Fig. 2) and follows a similar spatial distribution (Fig. 4). The spatial distribution of gases in the cave is dependent of the air density gradient between the cave and the outer atmosphere and controls the seasonal variations of both tracer gases. These processes affect the entire air masses, without distinguishing between single gases. In addition, the diffusion coefficients in the air of CO₂ and ²²²Rn are similar. For instance, at 25 °C, they are 0.15·10^–4 m²/s and 0.12·10^–4 m²/s for CO₂ and ²²²Rn, respectively (Lerman 1979; Nazaroff 1992). Thus, maximum concentrations of both gases are reached simultaneously, confirming the annual dependency of gas concentrations on the temperature gradient, and thus on the predominance of gaseous diffusion or advection.

Production and exhalation of ²²²Rn depend primarily on the host rock, speleothems and sediments of the cave, and also on the soil above it. ²²²Rn derives from radium decay and its emanation rate and transport through the porous networks are dependent on the different environmental and geological conditions (Álvarez-Gallego et al. 2015; Lario et al. 2005).

²²²Rn produced in soil and host rock migrates within the rock mass (Ferry et al. 2002) by diffusion and/or advection through pores, macro-pores, and fractures (Ajayi et al. 2018; Ferry et al. 2002; Nazaroff and Nero 1988), reaching the cave indoors. Transport of ²²²Rn and CO₂ dissolved in water increases when soil is saturated. Under this situation, water permeability becomes critical in the gas transport. Both gases are soluble in water and present similar solubility: at 25 °C, the fraction solubility of CO₂ and ²²²Rn is 6.15·10^–4 and 1.67·10^–4, respectively (Crovetto 1991; IUPAC 1979) and diffusion coefficient in water is 1.95·10^–9 m²/s and 1.37·10^–9 m²/s for CO₂ and ²²²Rn, respectively (Lerman 1979; Nazaroff 1992). Thus, waters with dissolved ²²²Rn and CO₂ can flow through pores and fractures of the host-rock and enter the cave, where the degasification of dripping waters can contribute to increase the gaseous concentration.

The ²²²Rn measured in Rull soil emanates into the atmosphere with a particular rate, inherent to the soil composition and nature of the samples (Amin 2015; Amin et al. 2008). Exhalation in Rull soil might be related to a particular characteristic rate but the variations from 0.05 to 0.005 Bq/m²s (Fig. 7) are a consequence of the environmental variables and soil conditions. The soil water content affects soil ²²²Rn concentration mainly through its influence on the radon emanation coefficient in soil (Benavente et al. 2019; Sun et al. 2004). Water content in the soil promotes ²²²Rn exhalation up to a certain level of this water content and retains the ²²²Rn afterwards (Yang et al. 2019). Thus, maximum values of ²²²Rn concentration in the soil during spring followed by a temporal delay of maximum exhalation rates in Rull soil may be mainly related to the variations in soil water content due to rainfalls. Previous studies in different field sites (Kojima and Nagano 2005; Megumi and Mamuro 1973; Schery et al. 1984) confirmed that the radon exhalation rate did not change significantly with light rainfall (13 mm), but it decreased dramatically with heavy rainfall (93 mm) and remained low for several days after heavy rainfall.

²²²Rn produced in soil depends on uranium and thorium concentration, size particle (texture), and temperature and water content, which affect the emanation coefficient and it is a consequence of rainfall and water condensation (Fig. 7). Although the time series of ²²²Rn soil measurements is shorter compared to the CO₂ soil measurements, ²²²Rn concentration in soil (discrete measurements) increases significantly when rainfall occurs. A pattern similar to the one described by soil CO₂ is repeated by soil ²²²Rn. The rainfall occurred between March and May in 2022 is coincident with the maximum measured ²²²Rn concentration in soil. Maximum values of ²²²Rn are reached before maximum CO₂, but this delay might be related to the different origin of each gas. In Rull site, the ²²²Rn emitted by the soil is also transported by diffusion into the cave. The consequence of this process is a rising in ²²²Rn in the cave atmosphere, which strengthens in absence of ventilation.

The uranium and thorium concentration in the materials of the soil-cave system depends on their mineralogical composition and specifically on clay content. As described previously, soil above the cave and cave sediment present a higher uranium and thorium concentration. Moreover, both soil and cave sediments are powder materials, and the emanation is enhanced in comparison to host-rock and speleothems, although their volume in the cave is higher than soil and cave sediments.

Part of the ²²²Rn produced by soil migrates into the cave by diffusion since soil ²²²Rn concentration is higher than in the cave (Fig. 7) as occurs with CO₂. ²²²Rn transport from soil behaves similarly to CO₂. However, due to the radioactive nature of ²²²Rn, its concentration shall decrease over time once the diffusion became negligible. In this situation, the emanation of ²²²Rn from cave sediments, host-rock and speleothems, which might be constant through the year (due to the microenvironmental conditions of the cave), contributes to increase ²²²Rn cave concentration. Furthermore, the ²²²Rn concentration in the cave is also dependent on the previously described ventilation mechanisms governing the cave dynamics (Fig. 2), which will be responsible of the lowest gaseous concentration.

In addition, although it has not been quantified for this study, dripping water degassing might also contribute to increasing ²²²Rn concentration in caves. ²²²Rn in dripping water is also related to precipitation that percolates through the soil-cave profile and depends on its residence time within the profile (Nakasone et al. 2021). Previous studies in other caves have highlighted that, at certain gaseous stages, dripping water degassing was responsible for the increases in ²²²Rn concentration of cave atmosphere (Tang et al. 2020). However, in Rull cave dripping waters are not abundant (Pla et al. 2020) and thus the contribution of CO₂ or ²²²Rn degassing from seepage waters to increase cave gaseous concentration might be low.

Time series recorded in Rull cave are useful to estimate adequate and healthy exposition to the gaseous atmosphere, considering the cave workers and visitors (the site receives an annual average of 15,000 visitors). In Rull cave, for the study period (November 2012–July 2022), maximum values obtained from daily average concentrations of CO₂ and ²²²Rn were 3966 ppm and 4185 Bq/m³, respectively. The EH40/2005 (2011) establishes for CO₂ a long-term exposure limit (8 h) of 5000 ppm at the workplace. Thus, workers and visitors’ exposition to CO₂ is considered safe since they never exceed this limit.

In addition, for ²²²Rn, ICRP (2017) recommends, for the specific situations of indoor work involving exposures in tourist caves, a dose coefficient of 6 mSv per mJ h/m³ which is equivalent, approximately, to 20 mSv per WLM (ICRP 2017). Complementary, national legislation (RD 783/2001 2001; CSN, Instrucción IS-33 2012) establishes that workers receiving radiation due to their work activity higher than 6 mSv/year must undergo strict physical examinations. Consequently, it would be recommended, particularly, for the Rull cave workers not to surpass this threshold.

Between 2013 and 2014, the total time that guides spent inside the cave was monitored daily. Data from these years could be adopted as representative of the ordinary annual cycle since conditions have not changed significantly since then. Afterwards, average monthly individual times were obtained considering (1) that there are 3 permanent guides alternating visits inside the cave and (2) the variability in the number of visits (as time inside the cave) within a year (Fig. 2b). Attending to this, Table 1 shows the calculated effective dose received in a regular year by an individual guide.

The calculated dose is 5.97 mSv/year which is near to the recommended maximum exposure (6 mSv/year) for which national legislation indicates special control measures for workers. With an accurately planification of the visits, particularly when the cave is in the stagnation stage, workers could be less exposed to radiation. Microclimatic monitoring of Rull cave assists in the determination of potentially hazardous concentrations, to comply with the official air quality standards.