Diversity and geographic distribution of desmids and other coccoid green algae

Problems concerning species definition in microalgae considerably hamper the interpretation of algal biodiversity and biogeography. The morphologic species concept considers species as groups of morphologically identical or similar organisms (Futuyma 1998). The biologic species concept defines species as groups of interbreeding populations which are reproductively isolated from other groups (Mayr 1942). However, both species concepts give problems when applied to algal species. In this context, John and Maggs (1997) come to the conclusion that at present no operational species concept is available for eukaryotic algae.

Traditional, morphology-based desmid taxonomy is overloaded with synonyms and suffers from a high rate of splitting (see Diversity chapter below). Unfortunately, sexual reproduction—essential for applying the biologic species concept—is a relatively rare phenomenon in this algal group (of many species no sexual stages are known at all). In addition, desmids are haploid organisms, so most mutations are immediately expressed. Consequently, by predominant lack of sexual reproduction (so possible exchange of genes during meiotic cell division) genotypically determined morphological variation is not wiped out. Therefore, exclusively clonal reproduction (particularly in euplanktic species) may readily result in the formation of desmid microspecies, just like in apomictically reproducing macrophytes (Coesel and Joosten 1996).

One of the possible reasons that sexual reproduction stages (zygospores) are usually encountered only incidentally is that but few clonal populations are homothallic (i.e., self-fertile). In experiments, out of some 120 randomly selected desmid strains belonging to 16 genera and over 80 species, only three showed homothallic sexual reproduction (Coesel and Teixeira 1974). On the other hand, mating experiments between different clones, often originating from different sites, revealed the phenomenon of heterothallism in, e.g., Closterium ehrenbergii Ralfs (Ichimura 1981; Coesel 1988), Closterium strigosum Bréb. (Watanabe and Ichimura 1978), Pleurotaenium mamillatum West (Ling and Tyler 1976) and Micrasterias thomasiana Archer (Blackburn and Tyler 1987). Especially in Closterium ehrenbergii (Figs. 6, 7), many mating types have been demonstrated, that is, populations which mutually show (almost) complete sexual isolation (Ichimura 1981). Such mating types can be considered syngens or biological species. Often, but not necessarily, mating types of one and the same morphospecies slightly differ in morphology, ecology and/or geographical distribution (Ichimura and Kasai 1990; Ichimura et al. 1997). No doubt, such sibling species—also to be traced from DNA analyses (Denboh et al. 2003)—will occur in many more morphospecies, and it is clear that they will substantially increase the diversity of this algal group.

Also in chlorococcal algal taxonomy, the morphologic species concept is burdened with a high degree of misinterpretation. For example, one and the same morphotype, such as the globular ‘green ball’, has evolved in different phylogenetic lineages. On the other hand, highly diverse morphotypes can belong to one and the same lineage (Luo et al. 2005). This ambivalent interpretation of morphotypes was documented on the archetypical green ball Chlorella and relatives. In freshwater and brackish habitats several distinct lineages of spherical ‘Chlorella-like’ green algae have been found and designated as separate clades: Mychonastes/Pseudodictyosphaerium, now placed in the Chlorophyceae; and Choricystis/Nanochlorum/Chlorella, now placed in the Trebouxiophyceae (Krienitz et al. 1999; Hepperle and Krienitz 2001). In marine habitats, several other lineages of globular green algae have been reported, e.g., in the prasinophytes Pycnococcus provasolii Guillard, now placed in the Pseudoscourfieldiales (Daugbjerg et al. 1995); and Ostreococcus tauri Courties et Chrétiennot-Dinet, now placed in the Mamiellales (Courties et al. 1998). Further members of the Chlorella morphotype await taxonomic treatment and a transfer to other lineages of the Chlorophyta (Komárek and Fott 1983). The multiple origin of ‘Chlorella-like’ algae may be explained by an adaptive advantage of the coccoid morphotype in ecosystems (Potter et al. 1997). On the other hand, sequence analyses revealed that several morphologically different algae, that were grouped in different lineages, such as Closteriopsis, Actinastrum, Dictyosphaerium, Didymogenes and Micractinium cluster close to globular ‘true’ Chlorella sensu stricto species (Fig. 14).

In numerous chlorococcal algal species sexual reproduction is unknown. Therefore, the biologic species concept is not applicable. Fortunately, molecular analyses offer an alternative to get insight in their relationships. After the introduction of DNA sequencing and phylogenetic analyses, the systematics of algae is going through a dramatic phase of change. The recent situation is marked by the quest for a compromise between the conventional (artificial) and the phylogenetic system. This upheaval will have significant consequences on biodiversity and biogeography of algal taxa. Some examples of these changing views will be given in the following two chapters.

According to Gerrath (1993), estimations of global desmid species richness highly vary, but for the most part will be too high in view of the fair number of current synonyms. To give here a single example of possible synonymy: Cosmarium seelyanum Wolle, C. wallichii West and West, C. divergens Krieg., C. naivashensis Rich, C. nobile (Turner) Krieg., and C. subnobile Hinode are possibly the same species (Figs. 8–13), which occur in the literature also under the name of C. abruptum Lundell. Minor morphologic differences often are within the phenotypic variability of a species, and are thus not of taxonomic significance. Many forms, at any time described as separate varieties or even species, appear to be interconnected by transitional forms, so most likely belong to one and the same taxon, e.g, the common species Staurastrum furcatum (Ralfs) Bréb. and Staurastrum aciculiferum (West) Andersson (Figs. 15–17). However, such causes for overestimation of species diversity are counterbalanced by reasons for underestimation. Different species the vegetative cells of which are very much alike in practice usually will not be recognized. Certain small-sized, smooth-walled Cosmarium species can only be identified reliably when zygospores are encountered (Figs. 18–23). As zygospores usually are met only incidentally, new species can be expected on account of zygospore morphology. Apart from possible under- and overestimations of species diversity, related to insufficient observations or failures in knowledge of relevant literature, a reliable assessment of species diversity is also hindered by the experience that polymorphism in a ‘species’ is not always phenotypic but also may have got a genetic base, like in sibling species.

Taking into account all these complications it is fully understandable that estimations of total desmid species richness in (rather recent) literature range from 1,500 to 12,000 (Gerrath 1993). Gerrath (1993) thinks that there are approximately 3,000 ‘good’ desmid species worldwide. When extrapolating the number of morphospecies distinguished in an ongoing inventory of European Staurastra, the first author comes to a comparable number. Anyhow, it is remarkable that hardly any new desmid flagship species are found. A recent, rather extensive investigation in northern Australia revealed but a single ‘brand-new’ species, quite different from all taxa described before (Fig. 24). This could be an indication that the number of desmid species endemic to a relatively small geographical area is limited, which for a group of readily to be transported micro-organisms is not really surprising.

The most recent comprehensive monograph on chlorococcal algae (Komárek and Fott 1983) contains about 1,200 species and subspecific taxa. Estimations of the real number of species are extremely vague because of the pending situation in species concepts. To illustrate this, the former genus Scenedesmus (now split into the genera Scenedesmus, Desmodesmus, Acutodesmus) may be used as an example. As a result of the high variability in morphologic characters (shape and organization of coenobia, spines, incrustations, cell wall ornamentations), more than 1,300 (morpho)species and subspecies have been desribed (Hegewald and Silva 1988). Studies using unialgal cultures to estimate the morphological variability revealed a severe overestimation of species number (Hegewald et al. 1990; Trainor 1998; Hegewald 1999). These observations were supported by molecular studies (Hegewald 2000). On the other hand, combined studies on fine structure and gene sequence (ITS2) of 22 clones identified as Desmodesmus costato-granulatus (Skuja) Hegewald indicated a higher diversity than expected. According to this interdisciplinary approach, it was split into five species (Vanormelingen et al. 2007).

Another example of the ambivalent situation in estimation of species diversity is Botryococcus braunii Kützing considered to be a ‘well-known’ microplanktont of inland waters, showing typical, large colonies like bunches of grape. Komárek and Marvan (1992) collected 74 populations of Botryococcus-like algae worldwide, studied their morphologic characteristics, and found 13 different morphotypes which fulfilled the species status according to the commonly used criteria in chlorococcal algal taxonomy. Unfortunately, molecular studies on these algae are deficient. The only known study on the phylogenetic placement of Botryococcus is supporting a polyphyletic origin (Senousy et al. 2004).

Also taxon delimitation within the Selenastraceae, a family of needle-shaped and lunate chlorococcal algae, has experienced considerable changes in recent times. Komárková-Legnerová (1969) and Marvan et al. (1984) provided revisions of the Selenastraceae based on morphotypes. Hindák (1984) described several new species which are of intermediate morphology with respect to described species, e.g., Monoraphidium intermedium Hindák as an intermediate taxon of M. griffithii (Berk.) Kom.-Legn. and M. obtusum (Korsh.) Kom.-Legn. The first molecular phylogenetic study on the Selenastraceae (Krienitz et al. 2001) revealed an intermixing of common members of the genera Ankistrodesmus, Monoraphidium and Selenastrum which contradicts the traditional way of circumscription of genera and species in this family. Finally, Fawley et al. (2005) discovered cryptic species in the Selenastraceae. It was found that isolates of one and the same morphotype can differ in 18S rDNA sequences, whereas isolates with identical or similar 18S rDNA sequence can exhibit different morphologies. These results give further arguments for the necessity of interdisciplinary work in algal systematics and diversity.

Examples of peculiar, well-defined distribution patterns in desmids were already shown by Donat (1926) and refined by Heimans (1969). Most striking is the occurrence of a number of flagship species that, within Europe, are characterized by a marked atlantic-subarctic distribution: Staurastrum elongatum Barker, S. maamense Archer, S. arctiscon (Ralfs) Lundell, S. cerastes Lundell, S. ophiura Lundell, S. brasiliense Nordst., and S. sexangulare (Bulnh.) Lundell (Figs. 25‐28). As we have to do with aquatic organisms, it is difficult to imagine which climatic factor(s) could be responsible for such a remarkable distribution pattern. Likely it is some ecological parameter, linked to the nearness of seawater, that is decisive for their occurrence. The above-mentioned species are also known from the North American continent, but their distribution over there seems to be less distinct (Prescott et al. 1982). Anyhow, even in the atlantic and subarctic regions of Europe none of these species is really common. From The Netherlands, out of the seven above-mentioned species, S. elongatum, S. ophiura, S. arctiscon, S. brasiliense and S. cerastes have been reported, but only from one or a few sites and only in the first half of the last century. Some of them were regularly found during a longer period, e.g., S. ophiura between 1912 and 1930. From the fact that none of these species succeeded in enlarging its regional area, it is suggested that ecological demands rather than climatic factors or dispersal abilities are limiting.

In Europe, versus atlantic-subarctic species, also continental desmid species can be distinguished. Striking examples are Cosmarium striolatum (Nägeli) Archer [synonymous with C. tesselatum (Delponte) Nordst.] and Euastrum germanicum (Schmidle) Krieg. (Figs. 29, 30). Both Cosmarium striolatum and Euastrum germanicum are widely distributed on the continent (Heimans 1969; Coesel 1978), but are absent from Great Britain (Brook and Williamson 1991). In this case, the lack might be attributed to the isolated position of the British Islands. A few decades ago, these species were extremely rare in The Netherlands, too. Yet, in recent years Dutch records, particularly of Euastrum germanicum, are remarkably increasing in number. Obviously, both species are advancing in western direction and it may be only a question of (relatively little) time that they reach England.

Possibly, the fast increasing number of Dutch records of Euastrum germanicum in the last decade has to do with increasing average year temperatures. In this context, also the expansion of another conspicuous Dutch desmid species in The Netherlands has to be stressed, i.e., of Micrasterias americana Ralfs (Fig. 31). Although The Netherlands have been intensively inventoried for desmids already from the beginning of the 20th century, this species was not recorded before 1952 (Heimans 1969). Since then the number of Dutch records steadily increased, but it is only in the last decade that it has become one of the most common Micrasterias species of The Netherlands, also found in disturbed habitats. This latter phenomenon, though, might be an indication of a changed genetic constitution enabling the filling of another (larger) ecological niche.

In contrast to the desmids, the chlorococcal algae are generally supposed to be ubiquitous and to have a cosmopolitan distribution. As such they would serve as a good example of Beijerinck’s metaphor, taken up by Baas-Becking and finally accentuated by Fenchel et al. (1997), Finlay (2002) and Fenchel and Finlay (2004): ‘in micro-organisms, everything is everywhere, the environment selects’. This statement has evoked a heated discussion focusing on the species concepts. In recent times, numerous articles have been published which contradict the ubiquity hypothesis (reviewed by Foissner 2006 and Logares 2006). For micro-algae this statement is still under disputation (Coleman 2002, Finlay and Fenchel 2002). In diatoms, Hillebrandt et al. (2001) revealed a decreasing similarity of species composition with increasing geographic distance, and thus they reject strict ubiquity of unicellular taxa. Coleman (2001) found local adaptation and endemism in phytoflagellates of the genera Pandorina and Volvulina.

As for the chlorococcal algae, there are indications of endemism both in classical, morphological and in modern, molecular approaches. Wille (1924) described the genus Soropediastrum which contains two species only found on the Kergueles. From the same Antarctic region Pediastrum marvillense Théréz. and Couté (Fig. 32) was discovered, which also seems to be endemic (Komárek and Jankovská (2001). Detailed studies on chlorococcal algae of Cuba demonstrated in 20% of the taxa slight morphological differences in comparison to the original descriptions based on material from the temperate zone (Komárek 1983; Comas 1996). The latter authors found 21 taxa only recorded from Cuba. Several morphospecies of Botryococcus studied by Komárek and Marvan (1992) are only known from a few localities, e.g., B. fernandoi Komárek and Marvan (Fig. 33). The large oocystacean Makinoella tosaensis Okada (Fig. 34) has been reported only from Japan and Korea (Hegewald et al. 1999). Amphikrikos variabilis Krienitz (Fig. 35), with a distinct pattern of cell wall incrustation, has been found only in swamps and rivers of Namibia (Krienitz 1998).

Though, there are also indications that several species enlarged their distribution area considerably during the last decades (‘invading species’), e.g., the formerly ‘tropical/subtropical’ species Desmodesmus perforatus (Lemmerm.) Hegewald and Pediastrum simplex Meyen. Nowadays, these species are encountered regularly in the temperate zone (Jeon and Hegewald 2006; Geissler and Kies 2003).

Slapeta et al. (2006) performed molecular analyses in the morphospecies Micromonas pusilla Butcher, a marine, picoplanktic prasinophyte. This morphospecies appeared to be a complex of morphologically indistinguishable phylogenetic lineages, representing cryptic species. Although some of these entities were shown to have a global, oceanic distribution, a more restricted distribution of other ones could not be precluded.