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Erschienen in:
1999 | OriginalPaper | Buchkapitel
Luminescent Lanthanide Chelates for Improved Resonance Energy Transfer and Application to Biology
verfasst von : P. R. Selvin
Erschienen in: Applied Fluorescence in Chemistry, Biology and Medicine
Verlag: Springer Berlin Heidelberg
Enthalten in: Professional Book Archive
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Fluorescence resonance energy transfer (FRET) is a technique widely used to measure the distance between two points which are separated by approximately 10–80 Å. Lanthanide-based RET (LRET) is a recent modification of the technique with a number of technical advantages, yet relies on the same fundamental mechanism — subject to careful interpretation of various terms. A number of excellent reviews on FRET have been written [14, 21–28]. A recent review of LRET has also appeared [9], as well as a summary of lanthanide luminescence [29] and its application to bio-assays [30], so here we provide only a brief summary of the relevant theory.