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Journal of Nanoparticle Research

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01.02.2014 | Research Paper

Mechanistic aspects of protein corona formation: insulin adsorption onto gold nanoparticle surfaces

verfasst von: Stefan Grass, Lennart Treuel

Erschienen in: Journal of Nanoparticle Research | Ausgabe 2/2014

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Abstract

In biological fluids, an adsorption layer of proteins, a “protein corona” forms around nanoparticles (NPs) largely determining their biological identity. In many interactions with NPs proteins can undergo structural changes. Here, we study the adsorption of insulin onto gold NPs (mean hydrodynamic particle diameter 80 ± 18 nm), focusing on the structural consequences of the adsorption process for the protein. We use surface enhanced Raman scattering (SERS) spectroscopy to study changes in the protein’s secondary structure as well as the impact on integrity and conformations of disulfide bonds immediately on the NP surface. A detailed comparison to SERS spectra of cysteine and cystine provides first mechanistic insights into the causes for these conformational changes. Potential biological and toxicological implications of these findings are also discussed.

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Titel: Mechanistic aspects of protein corona formation: insulin adsorption onto gold nanoparticle surfaces
verfasst von: Stefan Grass
Lennart Treuel
Publikationsdatum: 01.02.2014
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 2/2014
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2254-0

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