nach oben

Medical & Biological Engineering & Computing

Erschienen in:

22.07.2016 | Original Article

Microfluidic microscopy-assisted label-free approach for cancer screening: automated microfluidic cytology for cancer screening

verfasst von: Veerendra Kalyan Jagannadh, G. Gopakumar, Gorthi R. K. Sai Subrahmanyam, Sai Siva Gorthi

Erschienen in: Medical & Biological Engineering & Computing | Ausgabe 5/2017

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

Each year, about 7–8 million deaths occur due to cancer around the world. More than half of the cancer-related deaths occur in the less-developed parts of the world. Cancer mortality rate can be reduced with early detection and subsequent treatment of the disease. In this paper, we introduce a microfluidic microscopy-based cost-effective and label-free approach for identification of cancerous cells. We outline a diagnostic framework for the same and detail an instrumentation layout. We have employed classical computer vision techniques such as 2D principal component analysis-based cell type representation followed by support vector machine-based classification. Analogous to criminal face recognition systems implemented with help of surveillance cameras, a signature-based approach for cancerous cell identification using microfluidic microscopy surveillance is demonstrated. Such a platform would facilitate affordable mass screening camps in the developing countries and therefore help decrease cancer mortality rate.

Vorheriger Artikel Computational study for the effects of coil configuration on blood flow characteristics in coil-embolized cerebral aneurysm

Nächster Artikel Photoplethysmography and ultrasonic-measurement-integrated simulation to clarify the relation between two-dimensional unsteady blood flow field and forward and backward waves in a carotid artery

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 340 Zeitschriften

aus folgenden Fachgebieten:

Bauwesen + Immobilien
Business IT + Informatik
Finance + Banking
Management + Führung
Marketing + Vertrieb
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Bancroft JD (2008) Theory and practice of histological techniques. Elsevier Health Sciences, Amsterdam

Basiji DA, Ortyn WE, Liang L, Venkatachalam V, Morrissey P (2007) Cellular image analysis and imaging by flow cytometry. Clin Lab Med 27(3):653–670. doi:10.1016/j.cll.2007.05.008 CrossRefPubMedPubMedCentral

Caprio GD, Stokes C, Higgins JM, Schonbrun E (2015) Single-cell measurement of red blood cell oxygen affinity. Proc Natl Acad Sci USA 112(32):9984–9989. doi:10.1073/pnas.1509252112 CrossRefPubMedPubMedCentral

Cohen SJ, Punt CJA, Iannotti N, Saidman BH, Sabbath KD, Gabrail NY, Picus J, Morse M, Mitchell E, Miller MC, Doyle GV, Tissing H, Terstappen LWMM, Meropol NJ (2008) Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer. J Clin Oncol 26(19):3213–3221. doi:10.1200/JCO.2007.15.8923 CrossRefPubMed

Dey P (2010) Cancer nucleus: morphology and beyond. Diagn Cytopathol 38(5):382–390. doi:10.1002/dc.21234 PubMed

Goda K, Ayazi A, Gossett DR, Sadasivam J, Lonappan CK, Sollier E, Fard AM, Hur SC, Adam J, Murray C, Wang C, Brackbill N, Carlo DD, Jalali B (2012) High-throughput single-microparticle imaging flow analyzer. Proc Natl Acad Sci USA 109(29):11630–11635. doi:10.1073/pnas.1204718109 CrossRefPubMedPubMedCentral

Gopakumar G, Subrahmanyam GRKS, Siva GS (2014) Morphology based classification of leukemia cell lines: K562 and MOLT in a microfluidics based imaging flow cytometer. In: Proceedings of the 2014 Indian conference on computer vision, graphics and image processing, ICVGIP’14, Bangalore, India, December 14–18, 2014, pp 34:1–34:7. doi:10.1145/2683483.2683517

Gopakumar G, Jagannadh VK, Gorthi SS, Subrahmanyam GRKS (2016) Framework for morphometric classification of cells in imaging flow cytometry. J Microsc 261(3):307–319. doi:10.1111/jmi.12335 CrossRefPubMed

Gorthi SS, Schonbrun E (2012) Phase imaging flow cytometry using a focus-stack collecting microscope. Opt Lett 37(4):707–709. doi:10.1364/OL.37.000707 CrossRefPubMed

10.

Gorthi SS, Schaak D, Schonbrun E (2013) Fluorescence imaging of flowing cells using a temporally coded excitation. Opt Express 21(4):5164–5170. doi:10.1364/OE.21.005164 CrossRefPubMed

11.

Kachel V, Benker G, Lichtnau K, Valet G, Glossner E (1979) Fast imaging in flow: a means of combining flow-cytometry and image analysis. J Histochem Cytochem 27(1):335–341. doi:10.1177/27.1.374598 CrossRefPubMed

12.

Kay DB, Cambier JL, Wheeless LL (1979) Imaging in flow. J Histochem Cytochem 27(1):329–334. doi:10.1177/27.1.374597 CrossRefPubMed

13.

Kirby M, Sirovich L (1990) Application of the Karhunen–Loeve procedure for the characterization of human faces. IEEE Trans Pattern Anal Mach Intell 12(1):103–108. doi:10.1109/34.41390 CrossRef

14.

Nayar R (2014) Cytopathology in oncology. Springer, BerlinCrossRef

15.

Ng EX, Miller MA, Jing T, Lauffenburger DA, Chen CH (2015) Low-volume multiplexed proteolytic activity assay and inhibitor analysis through a pico-injector array. Lab Chip 15(4):1153–1159. doi:10.1039/C4LC01162G CrossRefPubMed

16.

Ng EX, Miller MA, Jing T, Chen CH (2016) Single cell multiplexed assay for proteolytic activity using droplet microfluidics. Biosens Bioelectron 81:408–414. doi:10.1016/j.bios.2016.03.002 CrossRefPubMed

17.

Otto O, Rosendahl P, Mietke A, Golfier S, Herold C, Klaue D, Girardo S, Pagliara S, Ekpenyong A, Jacobi A, Wobus M, Tpfner N, Keyser UF, Mansfeld J, Fischer-Friedrich E, Guck J (2015) Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nat Methods 12(3):199–202. doi:10.1038/nmeth.3281, 4 p following 202 CrossRefPubMed

18.

Schonbrun E, Gorthi SS, Schaak D (2012) Microfabricated multiple field of view imaging flow cytometry. Lab Chip 12(2):268–273. doi:10.1039/c1lc20843h CrossRefPubMed

19.

Schonbrun E, Di Caprio G, Schaak D (2013) Dye exclusion microfluidic microscopy. Opt Express 21(7):8793–8798. doi:10.1364/OE.21.008793 CrossRefPubMed

20.

Schonbrun E, Malka R, Di Caprio G, Schaak D, Higgins JM (2014) Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume. Cytometry A 85(4):332–338. doi:10.1002/cyto.a.22450 CrossRefPubMedPubMedCentral

21.

Solomon D (2003) Chapter 14: role of triage testing in cervical cancer screening. JNCI Monogr 2003(31):97–101CrossRef

22.

Stewart BW (2014) Wild C: World cancer report 2014. World Health Organization, Geneva

23.

Sung Y, Lue N, Hamza B, Martel J, Irimia D, Dasari RR, Choi W, Yaqoob Z, So P (2014) Three-dimensional holographic refractive-index measurement of continuously flowing cells in a microfluidic channel. Phys Rev Appl 1(1):014002. doi:10.1103/PhysRevApplied.1.014002 CrossRefPubMedPubMedCentral

24.

Terstappen LW, Johnsen S, Segers-Nolten IM, Loken MR (1990) Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 76(9):1739–1747PubMed

25.

Terstappen LW, Safford M, Knemann S, Loken M, Zurlutter K, Bchner T, Hiddemann W, Wrmann B (1992) Flow cytometric characterization of acute myeloid leukemia. Part II. Phenotypic heterogeneity at diagnosis. Leukemia 6(1):70–80PubMed

26.

Walts AE, Thomas P (2002) Endometrial cells and the AutoPap system for primary screening of cervicovaginal Pap smears. Diagn Cytopathol 27(4):232–237. doi:10.1002/dc.10175 CrossRefPubMed

27.

Wu J, Li J, Chan RK (2013) A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis. Opt Express 21(12):14474–14480. doi:10.1364/OE.21.014474 CrossRefPubMed

28.

Xia Y, Whitesides GM (1998) Soft lithography. Annu Rev Mater Sci 28(1):153–184. doi:10.1146/annurev.matsci.28.1.153 CrossRef

29.

Yan Y, Boey D, Ng LT, Gruber J, Bettiol A, Thakor NV, Chen CH (2016) Continuous-flow C. elegans fluorescence expression analysis with real-time image processing through microfluidics. Biosens Bioelectron 77:428–434. doi:10.1016/j.bios.2015.09.045 CrossRefPubMed

30.

Yang J, Zhang D, Frangi A, Yang JY (2004) Two-dimensional PCA: a new approach to appearance-based face representation and recognition. IEEE Trans Pattern Anal Mach Intell 26(1):131–137. doi:10.1109/TPAMI.2004.1261097 CrossRefPubMed

Titel: Microfluidic microscopy-assisted label-free approach for cancer screening: automated microfluidic cytology for cancer screening
verfasst von: Veerendra Kalyan Jagannadh
G. Gopakumar
Gorthi R. K. Sai Subrahmanyam
Sai Siva Gorthi
Publikationsdatum: 22.07.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Medical & Biological Engineering & Computing / Ausgabe 5/2017
Print ISSN: 0140-0118
Elektronische ISSN: 1741-0444
DOI: https://doi.org/10.1007/s11517-016-1549-y

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Springer Professional "Wirtschaft"

Weitere Artikel der Ausgabe 5/2017

Novel kinematic indices for quantifying upper limb ability and dexterity after cervical spinal cord injury

Photoplethysmography and ultrasonic-measurement-integrated simulation to clarify the relation between two-dimensional unsteady blood flow field and forward and backward waves in a carotid artery

Computational study for the effects of coil configuration on blood flow characteristics in coil-embolized cerebral aneurysm

Predicting depressed patients with suicidal ideation from ECG recordings

Correlation-based feature selection and classification via regression of segmented chromosomes using geometric features

A spectral element method with adaptive segmentation for accurately simulating extracellular electrical stimulation of neurons