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2018 | OriginalPaper | Buchkapitel

Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis

verfasst von : Tobias Baumann, Matthias Exner, Nediljko Budisa

Erschienen in: Synthetic Biology – Metabolic Engineering

Verlag: Springer International Publishing

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Abstract

To date, the two systems most extensively used for noncanonical amino acid (ncAA) incorporation via orthogonal translation are based on the Methanococcus jannaschii TyrRS/tRNA CUA Tyr and the Methanosarcina barkeri/Methanosarcina mazei PylRS/tRNA CUA Pyl pairs. Here, we summarize the development and usage of the pyrrolysine-based system for orthogonal translation, a process that allows for the recombinant production of site-specifically labeled proteins and peptides. Via stop codon suppression in Escherichia coli and mammalian cells, genetically encoded biomolecules can be equipped with a great diversity of chemical functionalities including click chemistry handles, post-translational modifications, and photocaged sidechains.

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Nächstes Kapitel Promoter and Terminator Discovery and Engineering
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Metadaten
Titel
Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis
verfasst von
Tobias Baumann
Matthias Exner
Nediljko Budisa
Copyright-Jahr
2018
Buch
Synthetic Biology – Metabolic Engineering

Print ISBN: 978-3-319-55317-7

Electronic ISBN: 978-3-319-55318-4

Copyright-Jahr: 2018

DOI
https://doi.org/10.1007/10_2016_37