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Erschienen in:

2016 | OriginalPaper | Buchkapitel

Probing the Cytotoxicity of Nanoparticles: Experimental Pitfalls and Artifacts

verfasst von : Jenny Domey, Lisa Haslauer, Ina Grau, Claudia Strobel, Melanie Kettering, Ingrid Hilger

Erschienen in: Measuring Biological Impacts of Nanomaterials

Verlag: Springer International Publishing

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Abstract

Throughout the last years, a huge variety of different nanoparticle formulations have been studied with the aim to assess their harmlessness in biological systems, to elucidate how the morphological features govern their impact on cells, and to develop cell labeling strategies for biomedical purposes. Most of such studies are based on the use of various cell viability assays. Interestingly, different results – even contradictory ones – have been observed between the groups, even though the respective nanoparticle formulations were more or less similar. One possible reason for such discrepancies is the occurrence of specific interactions between the nanoparticles and the ingredients of the respective cell viability assays. A similar situation can be encountered when researchers investigate the labeling of (stem) cells for biomedical purposes. Hereto, different labeling efficiencies were observed with the corresponding effects on cell viability and functionality. Therefore, the present review focuses on potential pitfalls and artifacts associated with the cytotoxicity evaluation of nanomaterials.

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Vorheriges Kapitel Characterization of Nanoparticles Under Physiological Conditions

Nächstes Kapitel Monitoring the Impact of Nanomaterials on Animal Cells by Impedance Analysis: A Noninvasive, Label-Free, and Multimodal Approach

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Titel: Probing the Cytotoxicity of Nanoparticles: Experimental Pitfalls and Artifacts
verfasst von: Jenny Domey
Lisa Haslauer
Ina Grau
Claudia Strobel
Melanie Kettering
Ingrid Hilger
Verlag: Springer International Publishing
Buch: Measuring Biological Impacts of Nanomaterials
Print ISBN: 978-3-319-24821-9

Electronic ISBN: 978-3-319-24823-3

Copyright-Jahr: 2016
DOI: https://doi.org/10.1007/11663_2013_8

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