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2020 | OriginalPaper | Buchkapitel

Production of 3D-Printed Tympanic Membrane Scaffolds as a Tissue Engineering Application

verfasst von : Elif Ilhan, Songul Ulag, Ali Sahin, Nazmi Ekren, Osman Kilic, Faik Nuzhet Oktar, Oguzhan Gunduz

Erschienen in: Bioinformatics and Biomedical Engineering

Verlag: Springer International Publishing

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Abstract

In recent years, scaffolds produced in 3D printing technology have become more widespread tool due to providing more advantages than traditional methods in tissue engineering applications. In this research, it was aimed to produce patches for the treatment of tympanic membrane perforations which caused significant hearing loss by using 3D printing method.

Polylactic acid (PLA) scaffolds with Chitosan (CS) added in various ratios were prepared for artificial eardrum patches. Different amounts of CS added to PLA to obtain more biocompatible scaffolds. The created patches were designed by mimicking the thickness of the natural tympanic membrane thanks to the precision provided by the 3D printed method. The produced scaffolds were analyzed separately for physical, chemical, morphological, mechanical and biocompatibility properties. Human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) were used for cell culture study to analyze the biocompatibility properties. 15 wt% PLA was chosen as the control group. Scaffold containing 3 wt% CS demonstrated significantly superior and favorable features in printing quality. The study continued with these two scaffolds (15PLA and 15PLA/3CS). This study showed that PLA and PLA/CS 3D printed scaffolds are a potential application for repairing tympanic membrane perforation.

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Titel: Production of 3D-Printed Tympanic Membrane Scaffolds as a Tissue Engineering Application
verfasst von: Elif Ilhan
Songul Ulag
Ali Sahin
Nazmi Ekren
Osman Kilic
Faik Nuzhet Oktar
Oguzhan Gunduz
Verlag: Springer International Publishing
Buch: Bioinformatics and Biomedical Engineering
Print ISBN: 978-3-030-45384-8

Electronic ISBN: 978-3-030-45385-5

Copyright-Jahr: 2020
DOI: https://doi.org/10.1007/978-3-030-45385-5_16

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