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01.03.2012 | Research Paper

Quantum dot bio-conjugate: as a western blot probe for highly sensitive detection of cellular proteins

verfasst von: Sonia Kale, Anup Kale, Haribhau Gholap, Abhimanyu Rana, Rama Desai, Arun Banpurkar, Satishchandra Ogale, Padma Shastry

Erschienen in: Journal of Nanoparticle Research | Ausgabe 3/2012

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Abstract

In the present study, we report a quantum dot (QD)-tailored western blot analysis for a sensitive, rapid and flexible detection of the nuclear and cytoplasmic proteins. Highly luminescent CdTe and (CdTe)ZnS QDs are synthesized by aqueous method. High resolution transmission electron microscopy, Raman spectroscopy, fourier transform infrared spectroscopy, fluorescence spectroscopy and X-ray diffraction are used to characterize the properties of the quantum dots. The QDs are functionalized with antibodies of prostate apoptosis response-4 (Par-4), poly(ADP-ribose) polymerases and β actin to specifically bind with the proteins localized in the nucleus and cytoplasm of the cells, respectively. The QD-conjugated antibodies are used to overcome the limitations of conventional western blot technique. The sensitivity and rapidity of protein detection in QD-based approach is very high, with detection limits up to 10 pg of protein. In addition, these labels provide the capability of enhanced identification and localization of marker proteins in intact cells by confocal laser scanning microscopy.

Vorheriger Artikel Performance of YAG:Eu3+, YAG:Tb3+ and BAM:Eu2+ plasma display nanophosphors

Nächster Artikel Electron microscopy investigations of the organization of cerium oxide nanocrystallites and polymers developed in polyvinylpyrrolidone-assisted polyol synthesis process

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Titel: Quantum dot bio-conjugate: as a western blot probe for highly sensitive detection of cellular proteins
verfasst von: Sonia Kale
Anup Kale
Haribhau Gholap
Abhimanyu Rana
Rama Desai
Arun Banpurkar
Satishchandra Ogale
Padma Shastry
Publikationsdatum: 01.03.2012
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 3/2012
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-012-0732-9

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