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2015 | OriginalPaper | Buchkapitel

15. Screening of Dual Defects Strain and Effects on l-Isoleucine Production in Escherichia coli NML

verfasst von : Linan Yu, Huiyan Liu, Haitian Fang, Qing Wu

Erschienen in: Advances in Applied Biotechnology

Verlag: Springer Berlin Heidelberg

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Abstract

Original strain Escherichia coli N12 with lifting the repression of l-isoleucine was mutated mutagenesis by diethylsulfate (DES), then l-isoleucine producing strain named E. coli NML were obtained. The effects of E. coli K12, N12, and NML in fermentation process on biomass, yield of l-isoleucine and glucose consumption rate were studied by carrying out fed-batch fermentation by analyzing the metabolic sysnthesis process. At 48 h, l-isoleucine production in the fermentation medium of N12 and NML was 2.25 and 4.64 g/L, respectively, and it was not detected in E. coli K12. Results show that mutant strains with dual genetic markers (Met⁻ + Leu⁻) can help with the synthesis of l-isoleucine, which also provide some frame of reference for the production of other amino acids.

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Vorheriges Kapitel The Comparative Study of Fermentation Capability of Wheat Beer Yeast

Nächstes Kapitel Effect of MIG1 Gene Deletion on Lactose Utilization in Lac+ Saccharomyces cerevisiae Engineering Strains

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Titel: Screening of Dual Defects Strain and Effects on l-Isoleucine Production in Escherichia coli NML
verfasst von: Linan Yu
Huiyan Liu
Haitian Fang
Qing Wu
Verlag: Springer Berlin Heidelberg
Buch: Advances in Applied Biotechnology
Print ISBN: 978-3-662-46317-8

Electronic ISBN: 978-3-662-46318-5

Copyright-Jahr: 2015
DOI: https://doi.org/10.1007/978-3-662-46318-5_15

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