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Journal of Nanoparticle Research
Erschienen in: Journal of Nanoparticle Research 4/2012

01.03.2012 | Research Paper

Size-mediated cytotoxicity of nanocrystalline titanium dioxide, pure and zinc-doped hydroxyapatite nanoparticles in human hepatoma cells

verfasst von: G. Devanand Venkatasubbu, S. Ramasamy, G. S. Avadhani, L. Palanikumar, J. Kumar

Erschienen in: Journal of Nanoparticle Research | Ausgabe 4/2012

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Abstract

Nanoparticles are highly used in biological applications including nanomedicine. In this present study, the interaction of HepG2 hepatocellular carcinoma cells (HCC) with hydroxyapatite (HAp), zinc-doped hydroxyapatite, and titanium dioxide (TiO2) nanoparticles were investigated. Hydroxyapatite, zinc-doped hydroxyapatite and titanium dioxide nanoparticles were prepared by wet precipitation method. They were subjected to isochronal annealing at different temperatures. Particle morphology and size distribution were characterized by X-ray diffraction and transmission electron microscope. The nanoparticles were co-cultured with HepG2 cells. MTT assay was employed to evaluate the proliferation of tumor cells. The DNA damaging effect of HAp, Zn-doped HAp, and TiO2 nanoparticles in human hepatoma cells (HepG2) were evaluated using DNA fragmentation studies. The results showed that in HepG2 cells, the anti-tumor activity strongly depend on the size of nanoparticles in HCC cells. Cell cycle arrest analysis for HAp, zinc-doped HAp, and TiO2 nanoparticles revealed the influence of HAp, zinc-doped HAp, and titanium dioxide nanoparticles on the apoptosis of HepG2 cells. The results imply that the novel nano nature effect plays an important role in the biomedicinal application of nanoparticles.
Vorheriger Artikel Multi-walled carbon nanotubes supported Cu-doped ZnO nanoparticles and their optical property
Nächster Artikel Formation of net-like patterns of gold nanoparticles in liquid crystal matrix at the air–water interface

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Metadaten
Titel
Size-mediated cytotoxicity of nanocrystalline titanium dioxide, pure and zinc-doped hydroxyapatite nanoparticles in human hepatoma cells
verfasst von
G. Devanand Venkatasubbu
S. Ramasamy
G. S. Avadhani
L. Palanikumar
J. Kumar
Publikationsdatum
01.03.2012
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 4/2012
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-012-0819-3

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