Skip to main content
Fachgebiete
Automobil + Motoren Bauwesen + Immobilien Business IT + Informatik Elektrotechnik + Elektronik Energie + Nachhaltigkeit Finance + Banking Management + Führung Marketing + Vertrieb Maschinenbau + Werkstoffe Versicherung + Risiko
DE
EN
Bücher
Zeitschriften
Themenseiten
Organisationspsychologie Projektmanagement Marketing Smart Manufacturing
Weitere Formate
Podcasts Webinare Technik Webinare Wirtschaft Kongresse Veranstaltungskalender Awards
Jetzt Einzelzugang starten
Zugang für Unternehmen
Referenzkunden
SLX-Digitalkonferenz: Zukunftswerkstatt 2024

Mehr Umsatz mit Top-Kontakten

Am 7. Mai erfahren Sie, wie Vertriebsteams Leadgenerierung, Leadnurturing und Leadstrategien effizient steuern können.
Erweiterte Suche
Anmelden
nach oben
Polymer Bulletin
Erschienen in: Polymer Bulletin 2/2019

19.06.2018 | Original Paper

Synthesis, characterization and biocompatible properties of novel silk fibroin/graphene oxide nanocomposite scaffolds for bone tissue engineering application

verfasst von: Mehdi Narimani, Abbas Teimouri, Zeinab Shahbazarab

Erschienen in: Polymer Bulletin | Ausgabe 2/2019

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

Novel three-dimensional porous silk fibroin/graphene oxide (SF/GO) nanocomposite scaffolds with different graphene oxide (GO) concentrations were prepared by using the freeze-drying technique. The obtained SF/GO scaffolds were characterized by thermogravimetric analysis, X-ray diffraction, scanning electron microscopy, Brunauer–Emmett–Teller isotherm and Fourier transform infrared spectroscopy techniques. The water absorption, compressive properties, porosity, degradation, biomineralization capability, cell attachment and cell viability of the composite scaffolds were studied as well. Cytocompatibility of the scaffolds was studied in vitro by employing the methylthiazoletetrazolium assay. The results showed that the presence of graphene oxide nanoparticles throughout the fibroin matrix led to an increase in water uptake and mechanical properties; at the same time, the porosity of the scaffolds was decreased. The cell adhesion results also indicated that human osteoblast cells (MG-63) could adhere to the surface of SF/GO nanocomposites and develop on them. These suggest that SF/GO nanocomposite scaffolds may be a good candidate for bone tissue engineering applications.
Vorheriger Artikel Development of gelatin hydrogel pads incorporated with Eupatorium adenophorum essential oil as antibacterial wound dressing
Nächster Artikel Plasticized jackfruit seed starch: a viable alternative for the partial replacement of petroleum-based polymer blends

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Jetzt informieren
Literatur
1.
Jayakumar R, Chennazhi KP, Srinivasan S, Nair SV, Furuike T, Tamura H (2011) Chitin scaffolds in tissue engineering. Int J Mol Sci 15:1876–1887CrossRef
2.
Rocchietta I, Fontana F, Simion M (2008) Clinical outcomes of vertical bone augmentation to enable dental implant placement: a systematic review. J Clin Periodontol 35:203–215CrossRefPubMed
3.
Wang M (2003) Developing bioactive composite materials for tissue replacement. Biomaterials 24:2133–2151CrossRefPubMed
4.
Hutmacher DW (2000) Scaffolds in tissue engineering bone and cartilage. Biomaterials 21:2529–2543CrossRefPubMed
5.
Yang S, Leong KF, Du Z, Chua CK (2001) The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng 7:679–689CrossRefPubMed
6.
Ryan G, Pandit A, Apatsidis DP (2006) Fabrication methods of porous metals for use in orthopaedic applications. Biomaterials 27:2651–2670CrossRefPubMed
7.
8.
Bensaıd W, Triffitt JT, Blanchat C, Oudina K, Sedel L, Petite H (2003) A biodegradable fibrin scaffold for mesenchymal stem cell transplantation. Biomaterials 24:2497–2502CrossRefPubMed
9.
Kim UJ, Park J, Kim HJ, Wada M, Kaplan DL (2005) Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin. Biomaterials 26:2775–2785CrossRefPubMed
10.
Singh BN, Panda NN, Mund R, Pramanik K (2016) Carboxymethyl cellulose enables silk fibroin nanofibrous scaffold with enhanced biomimetic potential for bone tissue engineering application. Carbohydr Polym 151:335–347CrossRefPubMed
11.
Meinel L, Karageorgiou V, Hofmann S, Fajardo R, Snyder B, Li C, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan DL (2004) Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Res 71:25–34CrossRef
12.
Luangbudnark W, Viyoch J, Laupattarakasem W, Surakunprapha P, Laupattarakasem P (2012) Properties and biocompatibility of chitosan and silk fibroin blend films for application in skin tissue engineering. World J Sci 2012:697201–697211CrossRef
13.
Bhardwaj N, Kundu SC (2011) Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications. Carbohydr Polym 85:325–333CrossRef
14.
She Z, Liu W, Feng Q (2010) Silk fibroin/chitosan/heparin scaffold: preparation, antithrombogenicity and culture with hepatocytes. Polym Int 59:55–61CrossRef
15.
Ruiz ON, Fernando KS, Wang B, Brown NA, Luo PG, McNamara ND, Vangsness M, Sun YP, Bunker CE (2011) Graphene oxide: a nonspecific enhancer of cellular growth. ACS Nano 5:8100–8107CrossRefPubMed
16.
Mehrali M, Moghaddam E, Shirazi SF, Baradaran S, Mehrali M, Latibari ST, Metselaar HS, Kadri NA, Zandi K, Osman NA (2014) Synthesis, mechanical properties, and in vitro biocompatibility with osteoblasts of calcium silicate–reduced graphene oxide composites. ACS Appl Mater Interface 6:3947–3962CrossRef
17.
Huang G, Chen S, Tang S, Gao J (2012) A novel intumescent flame retardant-functionalized graphene: Nanocomposite synthesis, characterization, and flammability properties. Mater Chem Phys 135:938–947CrossRef
18.
Kalbacova M, Broz A, Kong J, Kalbac M (2010) Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells. Carbon 48:4323–4329CrossRef
19.
Nayak TR, Andersen H, Makam VS, Khaw C, Bae S, Xu X, Ee PL, Ahn JH, Hong BH, Pastorin G, Ozyilmaz B (2011) Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 5:4670–4678CrossRefPubMed
20.
Zhang J, Zhang F, Yang H, Huang X, Liu H, Zhang J, Guo S (2010) Graphene oxide as a matrix for enzyme immobilization. Langmuir 26:6083–6085CrossRefPubMed
21.
Sui ZY, Cui Y, Zhu JH, Han BH (2013) Preparation of three-dimensional graphene oxide–polyethylenimine porous materials as dye and gas adsorbents. ACS Appl Mater Interface 5:9172–9179CrossRef
22.
Ruiz ON, Fernando KS, Wang B, Brown NA, Luo PG, McNamara ND, Vangsness M, Sun YP, Bunker CE (2011) Graphene oxide: a nonspecific enhancer of cellular growth. ACS Nano 5:8100–8107CrossRefPubMed
23.
Yoon OJ, Jung CY, Sohn IY, Kim HJ, Hong B, Jhon MS, Lee NE (2011) Nanocomposite nanofibers of poly(d, l-lactic-co-glycolic acid) and graphene oxide nanosheets. Compos Part A Appl Sci Manuf 42:1978–1984CrossRef
24.
Yoon OJ, Sohn IY, Kim DJ, Lee NE (2012) Enhancement of thermomechanical properties of poly (d, l-lactic-co-glycolic acid) and graphene oxide composite films for scaffolds. Macromol Res 1:1–6
25.
Wu S, Zhao X, Cui Z, Zhao C, Wang Y, Du L, Li Y (2014) Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells. Int J Nano Med 9:1413–1421
26.
Teimouri A, Ebrahimi R, Chermahini AN, Emadi R (2015) Fabrication and characterization of silk fibroin/chitosan/nano γ-alumina composite scaffolds for tissue engineering applications. RSC Adv 5:27558–27570CrossRef
27.
Teimouri A, Ebrahimi R, Emadi R, Beni BH, Chermahini AN (2015) Nano-composite of silk fibroin–chitosan/Nano ZrO2 for tissue engineering applications: Fabrication and morphology. Int J Biol Macromol 76:292–302CrossRefPubMed
28.
Ghorbanian L, Emadi R, Razavi SM, Shin H, Teimouri A (2013) Fabrication and characterization of novel diopside/silk fibroin nanocomposite scaffolds for potential application in maxillofacial bone regeneration. Int J Biol Macromol 58:275–280CrossRefPubMed
29.
Rockwood DN, Preda RC, Yücel T, Wang X, Lovett ML, Kaplan DL (2011) Materials fabrication from Bombyx mori silk fibroin. Nat Protoc 6:1612–1631CrossRefPubMed
30.
Lim HN, Huang NM, Lim SS, Harrison I, Chia CH (2011) Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth. Int J Nano Med 6:1817–1823CrossRef
31.
Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–2915CrossRefPubMed
32.
Escamilla-García M, Calderon-Dominguez G, Chanona-Perez JJ, Farrera-Rebollo RR, Andraca-Adame JA, Arzate-Vazquez I, Mendez-Mendez JV, Moreno-Ruiz LA (2013) Physical and structural characterisation of zein and chitosan edible films using nanotechnology tools. Int J Biol Macromol 61:196–203CrossRefPubMed
33.
Zdravkov B, Čermák J, Šefara M, Janků J (2007) Pore classification in the characterization of porous materials: a perspective. Open Chem 5:385–395CrossRef
34.
Alonso-Lemus I, Verde-Gómez Y, Álvarez-Contreras L (2011) Platinum nanoparticles synthesis supported in mesoporous silica and its effect in MCM-41 lattice. Int J Electrochem Sci 6:4176–4187
35.
Ho MH, Kuo PY, Hsieh HJ, Hsien TY, Hou LT, Lai JY, Wang DM (2004) Preparation of porous scaffolds by using freeze-extraction and freeze-gelation methods. Biomaterials 25:129–138CrossRefPubMed
36.
Zeltinger J, Sherwood JK, Graham DA, Müeller R, Griffith LG (2001) Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng 7:557–572CrossRefPubMed
37.
Wan Y, Chen X, Xiong G, Guo R, Luo H (2014) Synthesis and characterization of three-dimensional porous graphene oxide/sodium alginate scaffolds with enhanced mechanical properties. Mater Express 4:429–434CrossRef
38.
Li J, Dou Y, Yang J, Yin Y, Zhang H, Yao F, Wang H, Yao K (2009) Surface characterization and biocompatibility of micro-and nano-hydroxyapatite/chitosan-gelatin network films. Mater Sci Eng 29:1207–1215CrossRef
39.
Vacanti JP, Langer R (1999) Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet 354:32–34CrossRef
40.
Thein-Han WW, Misra RD (2009) Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomater 5:1182–1197CrossRefPubMed
41.
Kumar PS, Srinivasan S, Lakshmanan VK, Tamura H, Nair SV, Jayakumar R (2011) Synthesis, characterization and cytocompatibility studies of α-chitin hydrogel/nano hydroxyapatite composite scaffolds. Int J Biol Macromol 49:20–31CrossRefPubMed
42.
Liao KH, Lin YS, Macosko CW, Haynes CL (2011) Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. ACS Appl Mater interface 3:2607–2615CrossRef
43.
Song J, Gao H, Zhu G, Cao X, Shi X, Wang Y (2015) The preparation and characterization of polycaprolactone/graphene oxide biocomposite nanofiber scaffolds and their application for directing cell behaviors. Carbon 95:1039–1050CrossRef
44.
45.
Aliramaji S, Zamanian A, Mozafari M (2017) Super-paramagnetic responsive silk fibroin/chitosan/magnetite scaffolds with tunable pore structures for bone tissue engineering applications. Mater Sci Eng 70:736–744CrossRef
46.
Xie C, Lu X, Han L, Xu J, Wang Z, Jiang L, Wang K, Zhang H, Ren F, Tang Y (2016) Biomimetic mineralized hierarchical graphene oxide/chitosan scaffolds with adsorbability for immobilization of nanoparticles for biomedical applications. ACS Appl Mater Interface 8:1707–1717CrossRef
47.
Azadi M, Teimouri A, Mehranzadeh G (2016) Preparation, characterization and biocompatible properties of β-chitin/silk fibroin/nanohydroxyapatite composite scaffolds prepared using a freeze-drying method. RSC Adv 6:7048–7060CrossRef
48.
She Z, Zhang B, Jin C, Feng Q, Xu Y (2008) Preparation and in vitro degradation of porous three-dimensional silk fibroin/chitosan scaffold. Polym Degrad Stab 93:1316–1322CrossRef
49.
Bhardwaj N, Kundu SC (2011) Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications. Carbohydr Polym 85:325–333CrossRef
50.
Dinescu S, Ionita M, Pandele AM, Galateanu B, Iovu H, Ardelean A, Costache M, Hermenean A (2014) In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering. Bio-Med Mater Eng 24:2249–2256
51.
Wang L, Li C (2007) Preparation and physicochemical properties of a novel hydroxyapatite/chitosan–silk fibroin composite. Carbohydr Polym 68:740–745CrossRef
52.
Kim HS, Kim JT, Jung YJ, Ryu SC, Son HJ, Kim YG (2007) Preparation of a porous chitosan/fibroin-hydroxyapatite composite matrix for tissue engineering. Macromol Res 15:65–73CrossRef
Metadaten
Titel
Synthesis, characterization and biocompatible properties of novel silk fibroin/graphene oxide nanocomposite scaffolds for bone tissue engineering application
verfasst von
Mehdi Narimani
Abbas Teimouri
Zeinab Shahbazarab
Publikationsdatum
19.06.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Polymer Bulletin / Ausgabe 2/2019
Print ISSN: 0170-0839
Elektronische ISSN: 1436-2449
DOI
https://doi.org/10.1007/s00289-018-2390-2

Weitere Artikel der Ausgabe 2/2019

Polymer Bulletin 2/2019 Zur Ausgabe

Correction

Correction to: Potential of Borneo Acacia wood in fully biodegradable bio-composites’ commercial production and application

Original Paper

Ion conduction behavior of hot-pressed nanocomposite polymer electrolytes: (1 − x) [70PEO:30NaBr] + xSiO2

Original Paper

Baicalein-p-sulfonatocalix[n]arenes inclusion complexes: characterization, antioxidant ability and stability

Original Paper

Synthesis and characterization of acrylamide/(3-acrylamidopropyl) trimethyl ammonium chloride solution and acrylamide/Na-montmorillonite hydrogels via controlled radical polymerization for use as high-temperature and high-salinity oil reservoirs

Original Paper

Study of phase separation behavior of poly(N,N-diethylacrylamide) in aqueous solution prepared by RAFT polymerization

Original Paper

Development of gelatin hydrogel pads incorporated with Eupatorium adenophorum essential oil as antibacterial wound dressing

Premium Partner

    Marktübersichten

    Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen. 

    Zur Marktübersicht
    Bildnachweise