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2021 | OriginalPaper | Buchkapitel

7. Systematic Metabolic Engineering of Saccharomyces cerevisiae for Efficient Utilization of Xylose

verfasst von : Jing Han, Guoli Gong, Xia Wu, Jian Zha

Erschienen in: Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities

Verlag: Springer International Publishing

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Abstract

Efficient utilization of xylose is indispensable for economic biotransformation of biomass hydrolysates into fuels and other value-added chemicals. In the past two decades, there have been many metabolic engineering efforts in developing fast xylose-fermenting recombinant Saccharomyces cerevisiae strains, which are commonly used in industrial fermentation but are naturally incapable of metabolizing xylose. In this chapter, we review systematic metabolic engineering of S. cerevisiae to improve xylose metabolism via the xylose reductase (XR)-xylitol dehydrogenase (XDH) pathway or the xylose isomerase (XI) pathway. We focus on key regulatory targets that are highly involved in xylose metabolism, including redox imbalance caused by cofactor mismatch between XR and XDH, low activity of upper pathway enzymes (e.g., XR, XDH, XI, and xylulose kinase), inefficient xylose transportation, and incompatible sensing and signaling pathway. Meanwhile, we describe the construction of a new xylose metabolism pathway, the Weimberg pathway, in S. cerevisiae to metabolize xylose in a different manner, providing an option to synthesize more useful chemicals via the tricarboxylic acid cycle.

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Titel: Systematic Metabolic Engineering of Saccharomyces cerevisiae for Efficient Utilization of Xylose
verfasst von: Jing Han
Guoli Gong
Xia Wu
Jian Zha
Verlag: Springer International Publishing
Buch: Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities
Print ISBN: 978-3-030-65583-9

Electronic ISBN: 978-3-030-65584-6

Copyright-Jahr: 2021
DOI: https://doi.org/10.1007/978-3-030-65584-6_7