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Erschienen in: Measurement Techniques 12/2013

01.03.2013

The Method of Calibration Curves for Immunochromatographic Express Tests. Part 1. Immunochromatographic Express Tests with Colloidal Gold

verfasst von: S. S. Golubev, B. B. Dzantiev, A. V. Zherdev, Yu. V. Kiseleva, Ya. A. Korolenko, Yu. A. Kudeyarov, V. M. Malyuchenko, N. I. Smirnova, D. V. Sotnikov

Erschienen in: Measurement Techniques | Ausgabe 12/2013

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Abstract

The operating principle of immunochromatographic test systems is considered. It is noted that with the use of such tests an immunoassay is implemented as a sandwich method in the form of a direct dependence of the luminescence of test stripes on the concentration of the test substance or as a competitive method with an inverse dependence. An experimental procedure of obtaining such dependences, which is necessary for the construction of calibration curves, is set forth. Exponential expressions for arriving at an analytic description of the associated curves are proposed; moreover, the coefficients in these expressions are determined by the method of least squares. An analysis of the error of the method of calibration curves that may be used to obtain quantitative results of the analysis is presented.

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Fußnoten
1
See Fig. 1 of the article “Metrological assurance of systems of rapid medical diagnosis” by S. S. Golubev et al., Metrologiya, No. 10, 8 (2012).
 
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Metadaten
Titel
The Method of Calibration Curves for Immunochromatographic Express Tests. Part 1. Immunochromatographic Express Tests with Colloidal Gold
verfasst von
S. S. Golubev
B. B. Dzantiev
A. V. Zherdev
Yu. V. Kiseleva
Ya. A. Korolenko
Yu. A. Kudeyarov
V. M. Malyuchenko
N. I. Smirnova
D. V. Sotnikov
Publikationsdatum
01.03.2013
Verlag
Springer US
Erschienen in
Measurement Techniques / Ausgabe 12/2013
Print ISSN: 0543-1972
Elektronische ISSN: 1573-8906
DOI
https://doi.org/10.1007/s11018-013-0144-y

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