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Journal of Nanoparticle Research

Erschienen in:

01.12.2011 | Brief Communication

VEGFR-2 targeted cellular delivery of doxorubicin by gold nanoparticles for potential antiangiogenic therapy

verfasst von: Arijit Das, Eric Soehnlen, Stephan Woods, Ravi Hegde, Amanda Henry, Arne Gericke, Soumitra Basu

Erschienen in: Journal of Nanoparticle Research | Ausgabe 12/2011

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Abstract

We report a novel gold nanobioconjugate system that achieves targeted delivery of the small molecule drug doxorubicin to endothelial cells using anti-VEGFR-2 antibody conjugated gold nanoparticles (GNPs). The reported nanobioconjugate system combines the inherent ability of GNPs to undergo high levels of derivatization with the precision of antibody recognition of a cell surface antigen. Transmission electron microscopy (TEM) and surface-enhanced Raman spectroscopy (SERS) confirmed intracellular presence of the GNPs. Using a VEGFR-2 expressing cell line and a cell line that is negative for the receptor, in combination with competition assay we established the cell specific targeted delivery of the nanobioconjugate. The nanobioconjugate system described here may have potential drug delivery applications for antiangiogenic cancer therapy.

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Asadishad B, Vossoughi M, Alamzadeh I (2010a) In vitro release behavior and cytotoxicity of doxorubicin-loaded gold nanoparticles in cancerous cells. Biotechnol Lett 32(5):649–654CrossRef

Asadishad B, Vossoughi M, Alemzadeh I (2010b) Folate-receptor-targeted delivery of doxorubicin using polyethylene glycol-functionalized gold nanoparticles. Ind Eng Chem Res 49(4):1958–1963CrossRef

Bergen JM, Von Recum HA, Goodman TT, Massey AP, Pun SH (2006) Gold nanoparticles as a versatile platform for optimizing physicochemical parameters for targeted drug delivery. Macromol Biosci 6(7):506–516CrossRef

Chari RVJ (1998) Targeted delivery of chemotherapeutics: tumor-activated prodrug therapy. Adv Drug Deliv Rev 31(1–2):89–104CrossRef

Chen J, Wu H, Han D, Xie C (2006) Using anti-VEGF McAb and magnetic nanoparticles as double-targeting vector for the radioimmunotherapy of liver cancer. Cancer Lett 231(2):169–175CrossRef

Chen YH, Tsai CY, Huang PY, Chang MY, Cheng PC, Chou CH, Chen DH, Wang CR, Shiau AL, Wu CL (2007) Methotrexate conjugated to gold nanoparticles inhibits tumor growth in a syngeneic lung tumor model. Mol Pharm 4(5):713–722CrossRef

Chithrani BD, Ghazani AA, Chan WCW (2006) Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 6(4):662–668CrossRef

Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104(1):293–346CrossRef

Dixit V, Van den Bossche J, Sherman DM, Thompson DH, Andres RP (2006) Synthesis and grafting of thioctic acid-PEG-folate conjugates onto au nanoparticles for selective targeting of folate receptor-positive tumor cells. Bioconjug Chem 17(3):603–609CrossRef

Eliasson C, Loren A, Engelbrektsson J, Josefson M, Abrahamsson J, Abrahamsson K (2005) Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation. Spectrochim Acta A 61(4):755–760CrossRef

El-Sayed IH, Huang XH, El-Sayed MA (2005) Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer. Nano Lett 5(5):829–834CrossRef

Ganesh T (2007) Improved biochemical strategies for targeted delivery of taxoids. Bioorg Med Chem 15(11):3597–3623CrossRef

Ghosh SK, Pal A, Kundu S, Nath S, Pal T (2004) Fluorescence quenching of 1-methylaminopyrene near gold nanoparticles: size regime dependence of the small metallic particles. Chem Phys Lett 395(4–6):366–372CrossRef

Gibson JD, Khanal BP, Zubarev ER (2007) Paclitaxel-functionalized gold nanoparticles. J Am Chem Soc 129(37):11653–11661CrossRef

Gray GD, Basu S, Wickstrom E (1997) Transformed and immortalized cellular uptake of oligodeoxynucleoside phosphorothioates, 3′-alkylamino oligodeoxynucleotides, 2′-o-methyl oligoribonucleotides, oligodeoxynucleoside methylphosphonates, and peptide nucleic acids. Biochem Pharmacol 53(10):1465–1476CrossRef

Hillyer JF, Albrecht RM (2001) Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. J Pharm Sci 90(12):1927–1936CrossRef

Holder JM, Wynn-Williams DD, Perez FR, Edwards HGM (2000) Raman spectroscopy of pigments and oxalates in situ within epilithic lichens: Acarospora from the antarctic and mediterranean. New Phytol 145(2):271–280CrossRef

Hong R, Han G, Fernãndez JM, Kim B, Forbes NS, Rotello VM (2006) Glutathione-mediated delivery and release using monolayer protected nanoparticle carriers. J Am Chem Soc 128(4):1078–1079CrossRef

Huang XH, Jain PK, El-Sayed IH, El-Sayed MA (2006) Determination of the minimum temperature required for selective photothermal destruction of cancer cells with the use of immunotargeted gold nanoparticles. Photochem Photobiol 82(2):412–417CrossRef

Jaracz S, Chen J, Kuznetsova LV, Ojima L (2005) Recent advances in tumor-targeting anticancer drug conjugates. Bioorg Med Chem 13(17):5043–5054CrossRef

Kneipp J, Kneipp H, McLaughlin M, Brown D, Kneipp K (2006a) In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates. Nano Lett 6(10):2225–2231CrossRef

Kneipp K, Kneipp H, Kneipp J (2006b) Surface-enhanced Raman scattering in local optical fields of silver and gold nanoaggregatess—from single-molecule Raman spectroscopy to ultrasensitive probing in live cells. Acc Chem Res 39(7):443–450CrossRef

Liu CN, Tadayoni BM, Bourret LA, Mattocks KM, Derr SM, Widdison WC, Kedersha NL, Ariniello PD, Goldmacher VS, Lambert JM, Blattler WA, Chari RVJ (1996) Eradication of large colon tumor xenografts by targeted delivery of maytansinoids. Proc Natl Acad Sci USA 93(16):8618–8623CrossRef

Lu GW, Xia HR, Wang XQ, Xu D, Chen Y, Zhou YQ (2001) Raman scattering investigation of the zinc cadmium tetrathiocyanate single crystals. Mater Sci Eng B 87(2):117–121CrossRef

Oyelere AK, Chen PC, Huang XH, El-Sayed IH, El-Sayed MA (2007) Peptide-conjugated gold nanorods for nuclear targeting. Bioconjug Chem 18(5):1490–1497CrossRef

Paciotti GF, Kingston DGI, Tamarkin L (2006) Colloidal gold nanoparticles: a novel nanoparticle platform for developing multifunctional tumor-targeted drug delivery vectors. Chembiochem 67(1):47–54

Patra CR, Bhattacharya R, Wang E, Katarya A, Lau JS, Dutta S, Muders M, Wang S, Buhrow SA, Safgren SL, Yaszemski MJ, Reid JM, Ames MM, Mukherjee P, Mukhopadhyay D (2008) Targeted delivery of gemcitabine to pancreatic adenocarcinoma using cetuximab as a targeting agent. Cancer Res 68(6):1970–1978CrossRef

Roberts WG, Delaat J, Nagane M, Huang S, Cavenee WK, Palade GE (1998) Host microvasculature influence on tumor vascular morphology and endothelial gene expression. Am J Pathol 153(4):1239–1248CrossRef

Scott RC, Wang B, Nallamothu R, Pattillo CB, Perez-Liz G, Issekutz A, Del Valle L, Wood GC, Kiani MF (2007) Targeted delivery of antibody conjugated liposomal drug carriers to rat myocardial infarction. Biotechnol Bioeng 96(4):795–802CrossRef

Shao N, Lu S, Wickstrom E, Panchapakesan B (2007) Integrated molecular targeting of IGF1R and HER2 surface receptors and destruction of breast cancer cells using single wall carbon nanotubes. Nanotechnology 18(31):315101CrossRef

Speelmans G, Staffhorst RWHM, Steenbergen HG, de Kruijff B (1996) Transport of the anti-cancer drug doxorubicin across cytoplasmic membranes and membranes composed of phospholipids derived from Escherichia coli occurs via a similar mechanism. Biochim Biophys Acta (BBA) 1284(2):240–246CrossRef

Tkachenko AG, Xie H, Coleman D, Glomm W, Ryan J, Anderson MF, Franzen S, Feldheim DL (2003) Multifunctional gold nanoparticle–peptide complexes for nuclear targeting. J Am Chem Soc 125(16):4700–4701CrossRef

Witte L, Hicklin DJ, Zhu Z, Pytowski B, Kotanides H, Rockwell P, Böhlen P (1998) Monoclonal antibodies targeting the VEGF receptor-2 (flk1/KDR) as an anti-angiogenic therapeutic strategy. Cancer Metastasis Rev 17(2):155–161CrossRef

Yang PH, Sun XS, Chiu JF, Sun HZ, He QY (2005) Transferrin-mediated gold nanoparticle cellular uptake. Bioconjug Chem 16(3):494–496CrossRef

Yang T, Choi MK, Cui FD, Kim JS, Chung SJ, Shim CK, Kim DD (2007a) Preparation and evaluation of paclitaxel-loaded pegylated immunoliposome. J Control Rel 120(3):169–177CrossRef

Yang J, Lee CH, Ko HJ, Suh JS, Yoon HG, Lee K, Huh YM, Haam S (2007b) Multifunctional magneto-polymeric nanohybrids for targeted detection and synergistic therapeutic effects on breast cancer. Angew Chem Int Ed 46(46):8836–8839CrossRef

Titel: VEGFR-2 targeted cellular delivery of doxorubicin by gold nanoparticles for potential antiangiogenic therapy
verfasst von: Arijit Das
Eric Soehnlen
Stephan Woods
Ravi Hegde
Amanda Henry
Arne Gericke
Soumitra Basu
Publikationsdatum: 01.12.2011
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 12/2011
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-011-0300-8

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