nach oben

Erschienen in:

06.03.2022 | Original Research

An injectable chitosan-based hydrogel reinforced by oxidized nanocrystalline cellulose and mineral trioxide aggregate designed for tooth engineering applications

verfasst von: Elaheh Dalir Abdolahinia, Mahdieh Alipour, Marziyeh Aghazadeh, Mehdi Hassanpour, Marjan Ghorbani, Zahra Aghazadeh

Erschienen in: Cellulose | Ausgabe 6/2022

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

The complex anatomy of teeth limits the accessibility and efficacy of regenerative treatments. Therefore, the application of well-known inducers as injectable hydrogels for the regeneration of the dentin-pulp complex is considered a promising approach. In this regard, this study aimed to develop an injectable hydrogel containing mineral trioxide aggregate (MTA). The injectable chitosan/oxidized-nanocrystalline cellulose/MTA (CS/OCNC/MTA) hydrogels were prepared, and the physicochemical properties of these hydrogels were evaluated by TGA, FTIR, Rheological analysis, and SEM. Moreover, the effect of MTA on the swelling and degradability of scaffolds was assessed. The proliferative effects of synthesized hydrogels were also determined on human dental pulp stem cells (hDPSCs) by MTT assay. For induction of differentiation and biomineralization in these cells, the alkaline phosphatase activity and Alizarin Red S staining tests were performed in the presence of fabricated scaffolds. The proliferation of hDPSCs was significantly increased in the presence of these hydrogels. Moreover, the addition of MTA to hydrogel structure dramatically improved the differentiation of hDPSCs. These results suggested that this novel injectable hydrogel provides appropriate physiochemical properties and can be considered a promising scaffold for regenerative endodontic procedures.

Graphical abstract

Vorheriger Artikel Design of antibacterial cellulose nanofibril film by the incorporation of guanidine-attached lignin nanoparticles

Nächster Artikel Ionic liquid regenerated cellulose membrane electroless plated by silver layer for ECG signal monitoring

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Ahmadian S, Ghorbani M, Mahmoodzadeh F (2020) Silver sulfadiazine-loaded electrospun ethyl cellulose/polylactic acid/collagen nanofibrous mats with antibacterial properties for wound healing. Int J Biol Macromol 162:1555–1565. https://doi.org/10.1016/j.ijbiomac.2020.08.059CrossRefPubMed

Akbari A, Padervand M, Jalilian E, Seidi F (2020) Sodium alginate-halloysite nanotube gel beads as potential delivery system for sunitinib malate anticancer compound. Mater Lett 274:128038. https://doi.org/10.1016/j.matlet.2020.128038CrossRef

Akrami-Hasan-Kohal M, Tayebi L, Ghorbani M (2020) Curcumin-loaded naturally-based nanofibers as active wound dressing mats: Morphology, drug release, cell proliferation, and cell adhesion studies. New J Chem 44(25):10343–10351. https://doi.org/10.1039/d0nj01594fCrossRef

Alipour M, Pouya B, Aghazadeh Z, SamadiKafil H, Ghorbani M, Alizadeh S, Aghazadeh M, Dalir Abdolahinia E (2021) The antimicrobial, antioxidative, and anti-inflammatory effects of polycaprolactone/gelatin scaffolds containing chrysin for regenerative endodontic purposes. Stem Cells Int 2021:3828777. https://doi.org/10.1155/2021/3828777CrossRefPubMedPubMedCentral

Alipour M, Aghazadeh M, Ramezani S, Taherzadeh S, Ghorbani M, Aghazadeh Z (2022) Fabrication of a novel fibrous mat based on gliadin/ethylcellulose incorporated with triamcinolone for treatment of oral ulcers. J Polym Environ. https://doi.org/10.1007/s10924-021-02365-2CrossRef

Asl Aminabadi N, Satrab S, Najafpour E, Samiei M, Jamali Z, Shirazi S (2016) A randomized trial of direct pulp capping in primary molars using MTA compared to 3Mixtatin: a novel pulp capping biomaterial. Int J Paediatr Dent 26(4):281–290. https://doi.org/10.1111/ipd.12196CrossRefPubMed

Avani F, Damoogh S, Mottaghitalab F, Karkhaneh A, Farokhi M (2020) Vancomycin loaded halloysite nanotubes embedded in silk fibroin hydrogel applicable for bone tissue engineering. Int J Polym Mater Polym Biomater 69(1):32–43. https://doi.org/10.1080/00914037.2019.1616201CrossRef

Bendtsen ST, Wei M (2015) Synthesis and characterization of a novel injectable alginate–collagen–hydroxyapatite hydrogel for bone tissue regeneration. J Mater Chem B 3(15):3081–3090. https://doi.org/10.1039/C5TB00072FCrossRefPubMed

Burrus D, Barbeau L, Hodgson B (2014) Treatment of abscessed primary molars utilizing lesion sterilization and tissue repair: literature review and report of three cases. Pediatr Dent 36(3):240–244PubMed

Cao Y, Song M, Kim E, Shon W, Chugal N, Bogen G, Lin L, Kim RH, Park NH, Kang MK (2015) Pulp-dentin regeneration: current state and future prospects. J Dent Res 94(11):1544–1551. https://doi.org/10.1177/0022034515601658CrossRefPubMed

Chang B, Ahuja N, Ma C, Liu X (2017) Injectable scaffolds: preparation and application in dental and craniofacial regeneration. Mater Sci Eng R Rep 111:1–26. https://doi.org/10.1016/j.mser.2016.11.001CrossRefPubMedPubMedCentral

Domingues RMA, Silva M, Gershovich P, Betta S, Babo P, Caridade SG, Mano JF, Motta A, Reis RL, Gomes ME (2015) Development of injectable hyaluronic acid/cellulose nanocrystals bionanocomposite hydrogels for tissue engineering applications. Bioconjug Chem 26(8):1571–1581. https://doi.org/10.1021/acs.bioconjchem.5b00209CrossRefPubMed

Doustdar F, Olad A, Ghorbani M (2022) Development of a novel reinforced scaffold based on chitosan/cellulose nanocrystals/halloysite nanotubes for curcumin delivery. Carbohydr Polym 282:119127. https://doi.org/10.1016/j.carbpol.2022.119127CrossRefPubMed

Drabczyk A, Kudłacik-Kramarczyk S, Głąb M, Kędzierska M, Jaromin A, Mierzwiński D, Tyliszczak B (2020) Physicochemical investigations of Chitosan-based hydrogels containing aloe vera designed for biomedical use. Materials 13(14):3073. https://doi.org/10.3390/ma13143073CrossRefPubMedCentral

Fang J, Li P, Lu X et al (2019) A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration. Acta Biomat 88:503–513CrossRef

Gharib Khajeh H, Sabzi M, Ramezani S, Jalili AA, Ghorbani M (2022) Fabrication of a wound dressing mat based on Polyurethane/Polyacrylic acid containing Poloxamer for skin tissue engineering. Colloids Surf A Physicochem Eng Asp 633:127891. https://doi.org/10.1016/j.colsurfa.2021.127891CrossRef

Ghorbani M, Mahmoodzadeh F, Yavari Maroufi L, Nezhad-Mokhtari P (2020a) Electrospun tetracycline hydrochloride loaded zein/gum tragacanth/poly lactic acid nanofibers for biomedical application. Int J Biol Macromol 165(Pt A):1312–1322. https://doi.org/10.1016/j.ijbiomac.2020.09.225CrossRefPubMed

Ghorbani M, Nezhad-Mokhtari P, Ramazani S (2020b) Aloe vera-loaded nanofibrous scaffold based on Zein/Polycaprolactone/Collagen for wound healing. Int J Biol Macromol 153:921–930. https://doi.org/10.1016/j.ijbiomac.2020.03.036CrossRefPubMed

Ghorbani M, Roshangar L, Soleimani Rad J (2020) Development of reinforced chitosan/pectin scaffold by using the cellulose nanocrystals as nanofillers: An injectable hydrogel for tissue engineering. Eur Polym J 130:109697. https://doi.org/10.1016/j.eurpolymj.2020.109697CrossRef

Ghorbani M, Nezhad-Mokhtari P, Mahmoodzadeh F (2021) Incorporation of Oxidized Pectin to Reinforce Collagen/Konjac Glucomannan Hydrogels Designed for Tissue Engineering Applications. Macromol Res 29(4):289–296. https://doi.org/10.1007/s13233-021-9033-4CrossRef

Gregorio-Jauregui KM, Pineda MG, Rivera-Salinas JE, Hurtado G, Saade H, Martinez JL, Ilyina A, López RG (2012) One-step method for preparation of magnetic nanoparticles coated with Chitosan. J Nanomater 2012:813958. https://doi.org/10.1155/2012/813958CrossRef

Gregory CA, McNeill EP, Pan S (2020) Preparation of osteogenic matrices from cultured cells. Methods Cell Biol 156:15–43. https://doi.org/10.1016/bs.mcb.2019.10.009CrossRefPubMed

Gutmann JL (1992) The dentin-root complex: anatomic and biologic considerations in restoring endodontically treated teeth. J Prosthet Dent 67(4):458–467. https://doi.org/10.1016/0022-3913(92)90073-JCrossRefPubMed

Jimenez AS, Jaramillo F, Hemraz UD, Boluk Y, Ckless K, Sunasee R (2017) Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines. Nanotechnol Sci Appl 10:123–136. https://doi.org/10.2147/nsa.S145891CrossRefPubMedPubMedCentral

Jun S-K, Yoon J-Y, Mahapatra C, Park JH, Kim H-W, Kim H-R, Lee J-H, Lee H-H (2019) Ceria-incorporated MTA for accelerating odontoblastic differentiation via ROS downregulation. Dent Mater 35(9):1291–1299. https://doi.org/10.1016/j.dental.2019.05.024CrossRefPubMed

Jung C, Kim S, Sun T, Cho Y-B, Song M (2019) Pulp-dentin regeneration: current approaches and challenges. J Tissue Eng 10:2041731418819263. https://doi.org/10.1177/2041731418819263CrossRefPubMedPubMedCentral

Ko EK, Jeong SI, Rim NG, Lee YM, Shin H, Lee B-K (2008) In vitro osteogenic differentiation of human mesenchymal stem cells and in vivo bone formation in composite nanofiber meshes. Tissue Eng Part A 14(12):2105–2119. https://doi.org/10.1089/ten.tea.2008.0057CrossRefPubMed

Kumar A, Matari IAI, Choi H, Kim A, Suk YJ, Kim JY, Han SS (2019a) Development of halloysite nanotube/carboxylated-cellulose nanocrystal-reinforced and ionically-crosslinked polysaccharide hydrogels. Mater Sci Eng C Mater Biol Appl 104:109983. https://doi.org/10.1016/j.msec.2019.109983CrossRefPubMed

Kumar BYS, Isloor AM, Kumar GCM, Inamuddin AAM (2019b) Nanohydroxyapatite reinforced chitosan composite hydrogel with tunable mechanical and biological properties for cartilage regeneration. Sci Rep 9(1):15957. https://doi.org/10.1038/s41598-019-52042-7CrossRefPubMedPubMedCentral

Kumar A, Matari IAI, Han SS (2020) 3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering. Biofabrication 12(2):025029. https://doi.org/10.1088/1758-5090/ab736eCrossRefPubMed

Lungu A, Cernencu AI, Dinescu S, Balahura R, Mereuta P, Costache M, Syverud K, Stancu IC, Iovu H (2021) Nanocellulose-enriched hydrocolloid-based hydrogels designed using a Ca2+ free strategy based on citric acid. Mater Des 197:109200. https://doi.org/10.1016/j.matdes.2020.109200CrossRef

Mantha S, Pillai S, Khayambashi P, Upadhyay A, Zhang Y, Tao O, Pham HM, Tran SD (2019) Smart hydrogels in tissue engineering and regenerative medicine. Materials 12(20):3323. https://doi.org/10.3390/ma12203323CrossRefPubMedCentral

Margunato S, Taşlı PN, Aydın S, Karapınar Kazandağ M, Şahin F (2015) In vitro evaluation of ProRoot MTA, biodentine, and MM-MTA on human alveolar bone marrow stem cells in terms of biocompatibility and mineralization. J Endod 41(10):1646–1652. https://doi.org/10.1016/j.joen.2015.05.012CrossRefPubMed

Maroufi LY, Tabibiazar M, Ghorbani M, Jahanban-Esfahlan A (2021) Fabrication and characterization of novel antibacterial chitosan/dialdehyde guar gum hydrogels containing pomegranate peel extract for active food packaging application. Int J Biol Macromol 187:179–188. https://doi.org/10.1016/j.ijbiomac.2021.07.126CrossRefPubMed

Maturavongsadit P, Paravyan G, Shrivastava R, Benhabbour SR (2020) Thermo-/pH-responsive chitosan-cellulose nanocrystals based hydrogel with tunable mechanical properties for tissue regeneration applications. Materialia 12:100681. https://doi.org/10.1016/j.mtla.2020.100681CrossRef

Maturavongsadit P, Narayanan LK, Chansoria P, Shirwaiker R, Benhabbour SR (2021) Cell-Laden nanocellulose/chitosan-based bioinks for 3D bioprinting and enhanced osteogenic cell differentiation. ACS Appl Bio Mater 4(3):2342–2353. https://doi.org/10.1021/acsabm.0c01108CrossRefPubMed

Moon HJ, Lee JH, Kim JH, Knowles JC, Cho YB, Shin DH, Lee HH, Kim HW (2018) Reformulated mineral trioxide aggregate components and the assessments for use as future dental regenerative cements. J Tissue Eng 9:2041731418807396. https://doi.org/10.1177/2041731418807396CrossRefPubMedPubMedCentral

Moreno MA, Orqueda ME, Gómez-Mascaraque LG, Isla MI, López-Rubio A (2019) Crosslinked electrospun zein-based food packaging coatings containing bioactive chilto fruit extracts. Food Hydrocoll 95:496–505. https://doi.org/10.1016/j.foodhyd.2019.05.001CrossRef

Nam OH, Kim J-H, Choi SC, Kim Y (2020) Time-dependent response of human deciduous tooth-derived dental pulp cells treated with theracal LC: functional analysis of gene interactions compared to MTA. J Clin Med 9(2):531. https://doi.org/10.3390/jcm9020531CrossRefPubMedCentral

Nath J, Chowdhury A, Dolui SK (2018) Chitosan/graphene oxide-based multifunctional pH-responsive hydrogel with significant mechanical strength, self-healing property, and shape memory effect. Adv Polym Technol 37(8):3665–3679. https://doi.org/10.1002/adv.22151CrossRef

Nezhad-Mokhtari P, Ghorbani M, Abdyazdani N (2021) Reinforcement of hydrogel scaffold using oxidized-guar gum incorporated with curcumin-loaded zein nanoparticles to improve biological performance. Int J Biol Macromol 167:59–65. https://doi.org/10.1016/j.ijbiomac.2020.11.103CrossRefPubMed

Nuki K, Bonting SL (1961) Quantitative histochemistry of the developing hamster tooth: alkaline phosphatase and lactic dehydrogenase. J Histochem Cytochem 9:117–125. https://doi.org/10.1177/9.2.117CrossRefPubMed

Ogata K, Imazato S, Ehara A, Ebisu S, Kinomoto Y, Nakano T, Umakoshi Y (2005) Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites. J Biomed Mater Res A 72(2):127–135. https://doi.org/10.1002/jbm.a.30146CrossRefPubMed

Osuna Y, Gregorio-Jauregui KM, Gaona-Lozano JG, De La Garza-Rodríguez IM, Ilyna A, Barriga-Castro ED, Saade H, López RG (2012) Chitosan-coated magnetic nanoparticles with low chitosan content prepared in one-step. J Nanomater 2012:327562. https://doi.org/10.1155/2012/327562CrossRef

Patel DK, Dutta SD, Ganguly K, Lim K-T (2021) Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery. Int J Biol Macromol 170:178–188. https://doi.org/10.1016/j.ijbiomac.2020.12.145CrossRefPubMed

Pomari AAdN, Montanheiro TLdA, de Siqueira CP, Silva RS, Tada DB, Lemes AP (2019) Chitosan hydrogels crosslinked by genipin and reinforced with cellulose nanocrystals: production and characterization. J Compos Sci 3(3):84. https://doi.org/10.3390/jcs3030084CrossRef

Puchtler H, Meloan SN, Terry MS (1969) On the history and mechanism of alizarin and alizarin red S stains for calcium. J Histochem Cytochem 17(2):110–124. https://doi.org/10.1177/17.2.110CrossRefPubMed

Rath G, Hussain T, Chauhan G, Garg T, Goyal AK (2016) Development and characterization of cefazolin loaded zinc oxide nanoparticles composite gelatin nanofiber mats for postoperative surgical wounds. Mater Sci Eng C Mater Biol Appl 58:242–253. https://doi.org/10.1016/j.msec.2015.08.050CrossRefPubMed

Safari J, Azizi F, Sadeghi M (2015) Chitosan nanoparticles as a green and renewable catalyst in the synthesis of 1,4-dihydropyridine under solvent-free conditions. New J Chem 39(3):1905–1909. https://doi.org/10.1039/c4nj01730gCrossRef

Samiei M, Shirazi S, Azar FP, Fathifar Z, Ghojazadeh M, Alipour M (2019) The effect of different mixing methods on the properties of calcium-enriched mixture cement: a systematic review of in vitro studies. Iran Endod J 14(4):240–246. https://doi.org/10.22037/iej.v14i4.25126CrossRef

Saraç F, Saygılı F (2007) Causes of High Bone Alkaline Phosphatase. Biotechnol Biotec Eq 21(2):194–197. https://doi.org/10.1080/13102818.2007.10817444CrossRef

Sarker B, Papageorgiou DG, Silva R, Zehnder T, Gul ENF, Bertmer M, Kaschta J, Chrissafis K, Detsch R, Boccaccini AR (2014) Fabrication of alginate-gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. J Mater Chem B 2(11):1470–1482. https://doi.org/10.1039/c3tb21509aCrossRefPubMed

Schneider R, Holland GR, Chiego D Jr, Hu JC, Nör JE, Botero TM (2014) White mineral trioxide aggregate induces migration and proliferation of stem cells from the apical papilla. J Endod 40(7):931–936. https://doi.org/10.1016/j.joen.2013.11.021CrossRefPubMedPubMedCentral

Wang Q, Chen D (2016) Synthesis and characterization of a chitosan based nanocomposite injectable hydrogel. Carbohydr Polym 136:1228–1237. https://doi.org/10.1016/j.carbpol.2015.10.040CrossRefPubMed

Xue J, Niu Y, Gong M, Shi R, Chen D, Zhang L, Lvov Y (2015) Electrospun microfiber membranes embedded with drug-loaded clay nanotubes for sustained antimicrobial protection. ACS Nano 9(2):1600–1612. https://doi.org/10.1021/nn506255eCrossRefPubMed

Yavari Maroufi L, Ghorbani M (2021) Injectable chitosan-quince seed gum hydrogels encapsulated with curcumin loaded-halloysite nanotubes designed for tissue engineering application. Int J Biol Macromol 177:485–494. https://doi.org/10.1016/j.ijbiomac.2021.02.113CrossRefPubMed

Yavari Maroufi L, Ghorbani M, Tabibiazar M (2020) A gelatin-based film reinforced by covalent interaction with oxidized guar gum containing green tea extract as an active food packaging system. Food Bioprocess Technol 13(9):1633–1644. https://doi.org/10.1007/s11947-020-02509-7CrossRef

Yoshiki S, Umeda T, Kurahashi Y (1972) An effective reactivation of alkaline phosphatase in hard tissues completely decalcified for light and electron microscopy. Histochemie 29(4):296–304. https://doi.org/10.1007/BF00279812CrossRefPubMed

Yu F, Dong Y, Yang YW, Lin PT, Yu HH, Sun X, Sun XF, Zhou H, Huang L, Chen JH (2016) Effect of an experimental direct pulp-capping material on the properties and osteogenic differentiation of human dental pulp stem cells. Sci Rep 6:34713. https://doi.org/10.1038/srep34713CrossRefPubMedPubMedCentral

Zhang C, Ping Q, Zhang H, Shen J (2003) Synthesis and characterization of water-soluble O-succinyl-chitosan. Eur Polym J 39(8):1629–1634. https://doi.org/10.1016/S0014-3057(03)00068-5CrossRef

Zou Y, Zhang C, Wang P, Zhang Y, Zhang H (2020) Electrospun chitosan/polycaprolactone nanofibers containing chlorogenic acid-loaded halloysite nanotube for active food packaging. Carbohydr Polym 247:116711. https://doi.org/10.1016/j.carbpol.2020.116711CrossRefPubMed

Zubik K, Singhsa P, Wang Y, Manuspiya H, Narain R (2017) Thermo-responsive poly(N-isopropylacrylamide)-cellulose nanocrystals hybrid hydrogels for wound dressing. Polymers 9(4):119. https://doi.org/10.3390/polym9040119CrossRefPubMedCentral

Titel: An injectable chitosan-based hydrogel reinforced by oxidized nanocrystalline cellulose and mineral trioxide aggregate designed for tooth engineering applications
verfasst von: Elaheh Dalir Abdolahinia
Mahdieh Alipour
Marziyeh Aghazadeh
Mehdi Hassanpour
Marjan Ghorbani
Zahra Aghazadeh
Publikationsdatum: 06.03.2022
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 6/2022
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-022-04491-z

Springer Professional

Abstract

Graphical abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Weitere Artikel der Ausgabe 6/2022

The hopes and hypes of plant and bacteria-derived cellulose application in stem cell technology

Physical characteristics and cell-adhesive properties of in vivo fabricated bacterial cellulose/hyaluronan nanocomposites

Modified cotton fabric with durable anti-fouling performance for separation of surfactant-stabilized oil-in-water emulsions

Bio-derived efficient flame-retardants for cotton fabric

Continuous production of cellulose acetate microspheres for textile impregnation using a mesostructured reactor

Grafting amount and structural characteristics of microcrystalline cellulose functionalized with different aminosilane contents