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2012 | OriginalPaper | Buchkapitel

7.  Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering

verfasst von : Dr. Alexei Sorokin

Erschienen in: Bacillus thuringiensis Biotechnology

Verlag: Springer Netherlands

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Abstract

Experimental mapping of genes was less intensive in the Bacillus cereus group, which includes B. thuringiensis, than in Bacillus subtilis, generally considered as the model for Gram-positive bacteria. Nevertheless the genomic sequencing equalized densities of available gene maps. Moreover, the genes responsible for such complex phenomena like virulence or psychrotolerance could only be identified using the authentic bacteria prone to possess these properties. The new experimental approaches of post-genomic genetics should therefore be considered. Phage-mediated gene transduction and recombineering perspectives for the B. cereus group are reviewed. In combination with new generation sequencing these approaches will constitute the gene identification methodologies in the post-genomics time.

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Metadaten
Titel
Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering
verfasst von
Dr. Alexei Sorokin
Copyright-Jahr
2012
Verlag
Springer Netherlands
DOI
https://doi.org/10.1007/978-94-007-3021-2_7

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