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Erschienen in:

2019 | OriginalPaper | Buchkapitel

7. Collagen Gel Cell Encapsulation to Study the Effect of Fluid Flow on Mechanotransduction

verfasst von : Maryam Shariatzadeh, Cécile Perrault, Damien Lacroix

Erschienen in: Multiscale Mechanobiology in Tissue Engineering

Verlag: Springer Singapore

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Abstract

Mesenchymal stem cells (MSCs) are widely implicated for their potential use as a cell source for tissue engineering of skeletal tissue in regenerative medicine and tissue engineering. Mechanical forces from the microenvironment have a significant influence on differentiation of MSCs, and the resulting mechanotransduction would provide crucial adjuncts to standard biochemical signalling pathways. Combining microfluidic systems with mechanical stimulation for osteogenesis represents both a scientific and technological innovation that would greatly impact the field of regenerative medicine. We demonstrate a microfluidic chamber design for mechanical stimulation of flexible cellular microspheres and possibly a high-throughput microfluidic system for parallel processing of stem cell aggregation. We also showed that collagen microspheres serve an efficient cell delivery device supporting cell viability and migration post encapsulation.

A microfluidic chamber was made of PDMS with a central compression channel (0.6–0.9 mm). Bovine collagen type I with concentrations of 2 mg/ml and 2450 human embryonic mesenchymal stem progenitors (hES-MPs)/2.5 μl droplet was produced through gelation.

The microspheres were passed through the constriction at a flow rate of 320 μl/min for three cycles/day for 3 days starting at day 3 post encapsulation. Also effect of fluid sheer stress on the osteogenic differentiation of hES-MPs collagen microspheres was investigated using shaker and rocker systems. Cell viability, proliferation and bone markers alteration were monitored before and after compression.

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Vorheriges Kapitel Collagen Gel Cell Encapsulation to Study Mechanotransduction

Nächstes Kapitel Computational Modelling of Collagen Hydrogel

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Titel: Collagen Gel Cell Encapsulation to Study the Effect of Fluid Flow on Mechanotransduction
verfasst von: Maryam Shariatzadeh
Cécile Perrault
Damien Lacroix
Verlag: Springer Singapore
Buch: Multiscale Mechanobiology in Tissue Engineering
Print ISBN: 978-981-10-8074-6

Electronic ISBN: 978-981-10-8075-3

Copyright-Jahr: 2019
DOI: https://doi.org/10.1007/978-981-10-8075-3_7

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