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Erschienen in: Biomass Conversion and Biorefinery 16/2023

24.05.2022 | Original Article

Comprehensive metabolite profiling and therapeutic potential of black gram (Vigna mungo) pods: conversion of biowaste to wealth approach

verfasst von: Manikandan Arumugam, Dinesh Babu Manikandan, Sujitha Mohan, Arun Sridhar, Srinivasan Veeran, Sudharshini Jayaraman, Thirumurugan Ramasamy

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 16/2023

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Abstract

The risk faced by the drug-resistant pathogens, research, and development for viable alternative medicine is gaining traction. This study aims to utilize agricultural waste beneficially, by investigating the methanol, ethanol, acetone, ethyl acetate, petroleum ether, and hexane extracts of black gram pods by gas chromatography-mass spectrometry (GC–MS), and Fourier transform infrared (FT-IR) analysis to identify metabolites and functional groups and to evaluate its antibacterial and anti-biofilm potential on various fish disease-causing drug resistant pathogens like Aeromonas hydrophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Numerous compounds were identified as major peak area percentage by GC–MS analysis based on the polarity. Methanolic and ethanolic extracts of black gram pods showed higher phenolic and tannin content compared to other solvents, these results correlate with antioxidant potential. IC50 values of both 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) by the methanolic extracts possessed 933.807 and 976.285 µg/mL respectively. All the extracts possessed potential antibiofilm activity against A. hydrophila, K. pneumoniae, P. aeruginosa, and S. aureus in a dose-dependent manner. This study clearly shows that phenolic content is the major source for the inhibition of bacterial cell adherence (biofilm) against pathogens. Extraction in highly polar solvents exhibited higher content of phenols and tannins as compared to non-polar solvents. Findings of the current study support black gram pods as an excellent alternative medicine against fish disease-causing pathogens. It is proved in this study that the biowaste black gram pods could be recycled for the welfare of humans as well as for the growth of the country’s economy.

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Metadaten
Titel
Comprehensive metabolite profiling and therapeutic potential of black gram (Vigna mungo) pods: conversion of biowaste to wealth approach
verfasst von
Manikandan Arumugam
Dinesh Babu Manikandan
Sujitha Mohan
Arun Sridhar
Srinivasan Veeran
Sudharshini Jayaraman
Thirumurugan Ramasamy
Publikationsdatum
24.05.2022
Verlag
Springer Berlin Heidelberg
Erschienen in
Biomass Conversion and Biorefinery / Ausgabe 16/2023
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI
https://doi.org/10.1007/s13399-022-02806-5

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