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Erschienen in:
2013 | OriginalPaper | Buchkapitel
28. Construction of Recombinant Expression Vector of Anti-Bacterial Gene aiiA from Marine Bacterium
verfasst von : Xian Ding, Bo Yin, Shanfu Zhang, Weike Tang, Weiwen Sun, Shining Zhou
Erschienen in: Informatics and Management Science V
Verlag: Springer London
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Abstract
A pair of proper primers were designed and synthesized for Polymerase Chain Reaction (PCR) of anti-bacterial gene aiiA which encodes protein aiiA from marine bacterial genome, according to the gene sequences of aiiA. The plasmid pMD18-ZD02aiiA was constructed after PCR testing of aiiA gene. The gene sequence of aiiA was amplified with proper primers (with active locations by enzymes BamHIand EcoRI) from molding board of pMD18-ZD02aiiA and linked into expression vector pET-17b after it was hydrolyzed by enzymes of BamHI and EcoRI. And then plasmids pET-ZD02aiiA was constructed. The results of hydrolyzation of enzymes, PCR amplification and sequencing demonstrated that the target gene fragments were inserted into vector pET-17b correctly, the sequences of gene aiiA and frame of reading codes were right. Thus, it may provide base for the recombinant express and induced express of anti-bacterial gene from marine bacterium.