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Journal of Materials Science
Erschienen in: Journal of Materials Science 2/2018

16.10.2017 | Biomaterials

Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

verfasst von: Xue Li, Lei Tian, Zafar Ali, Wenyan Wang, Qiuyu Zhang

Erschienen in: Journal of Materials Science | Ausgabe 2/2018

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Abstract

Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.
Vorheriger Artikel High fluorescence of carbazole derivatives for bioimaging of endoplasmic reticulum and lysosome: the influence of molecular structure on optical properties and cell uptake
Nächster Artikel Effects of microstructural mechanisms on the localized oxidation behavior of NiTi shape memory alloys in simulated body fluid

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Metadaten
Titel
Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization
verfasst von
Xue Li
Lei Tian
Zafar Ali
Wenyan Wang
Qiuyu Zhang
Publikationsdatum
16.10.2017
Verlag
Springer US
Erschienen in
Journal of Materials Science / Ausgabe 2/2018
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-017-1581-9

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