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08.02.2023 | Original Article

Efficient extraction, excellent activity, and microencapsulation of flavonoids from Moringa oleifera leaves extracted by deep eutectic solvent

verfasst von: Ping Wei, Yue Zhang, Yao-Ying Wang, Jin-Feng Dong, Bi-Ni Liao, Zhi-Cheng Su, Wu Li, Ju-Cai Xu, Wen-Yong Lou, Hui-Hui Su, Chao Peng

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 16/2023

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Abstract

A deep eutectic solvent (choline chloride (ChCl)-urea) was chosen to extract flavonoids from Moringa oleifera leaves (FMOL), the condition of extraction was tailor-made, under the optimal extraction conditions (material-to-liquid ratio of 1:60 g/mL, extraction time of 80 min, extraction temperature of 80 °C), the highest extraction efficiency reached 63.2 ± 0.3 mg R/g DW, and nine flavonoids were identified. Then, the biological activities including antioxidant activities, antibacterial activities, and anti-tumor activities were systematically studied. FMOL was superior to positive drugs in terms of antioxidant activity. As to DPPH investigation, the IC₅₀ of FMOL and Vc were 64.1 ± 0.7 and 176.1 ± 2.0 µg/mL; for the ABTS, the IC₅₀ of FMOL and Vc were 9.5 ± 0.3 and 38.2 ± 1.2 µg/mL, the FRAP value of FMOL and Vc were 15.5 ± 0.6 and 10.2 ± 0.4 mg TE/g, and ORAC value of FMOL and Vc were 4687.2 ± 102.8 and 3881.6 ± 98.6 µmol TE/g. The bacteriostatic (MICs were ≤ 1.25 mg/mL) activities of FMOL were much better than propyl p-hydroxybenzoate. Meanwhile, FMOL had comparable inhibitory activity with genistein on tumor cells, IC50 was 307.8 µg/mL, and could effectively induce apoptosis in HCT116. Microcapsules were prepared with xylose-modified soybean protein isolate and gelatin as wall materials; after that, the intestinal release of modified FMOL microcapsules was 86 times of free FMOL. Therefore, this study confirmed that FMOL extracted with ChCl/urea has rich bioactive components, and microencapsulated FMOL has potential application in food industry.

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Titel: Efficient extraction, excellent activity, and microencapsulation of flavonoids from Moringa oleifera leaves extracted by deep eutectic solvent
verfasst von: Ping Wei
Yue Zhang
Yao-Ying Wang
Jin-Feng Dong
Bi-Ni Liao
Zhi-Cheng Su
Wu Li
Ju-Cai Xu
Wen-Yong Lou
Hui-Hui Su
Chao Peng
Publikationsdatum: 08.02.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Biomass Conversion and Biorefinery / Ausgabe 16/2023
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-023-03877-8

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