Skip to main content
Erschienen in: Journal of Nanoparticle Research 5/2009

01.07.2009 | Technology and Applications

Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels

verfasst von: Lan Yuan, Wei Wei, Juan Li, Zhiwei Sun, Hongfang Wang, Xiuzhi Zhang, Yueyue Chen

Erschienen in: Journal of Nanoparticle Research | Ausgabe 5/2009

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Anhänge
Nur mit Berechtigung zugänglich
Literatur
Zurück zum Zitat Alschinger M, Maniak M, Stietz F, Vartanyan T, Trager F (2003) Application of metal nanoparticles in confocal laser scanning microscopy: improved resolution by optical field enhancement. Appl Phys B 76:771–774. doi:10.1007/s00340-003-1182-y CrossRefADS Alschinger M, Maniak M, Stietz F, Vartanyan T, Trager F (2003) Application of metal nanoparticles in confocal laser scanning microscopy: improved resolution by optical field enhancement. Appl Phys B 76:771–774. doi:10.​1007/​s00340-003-1182-y CrossRefADS
Zurück zum Zitat Bhattacharya R, Patra CR, Verma R, Kumar S, Greipp PR, Mukherjee P (2007) Gold nanoparticles inhibit the proliferation of multiple myeloma cells. Adv Mater 19:711–716. doi:10.1002/adma.200602098 CrossRef Bhattacharya R, Patra CR, Verma R, Kumar S, Greipp PR, Mukherjee P (2007) Gold nanoparticles inhibit the proliferation of multiple myeloma cells. Adv Mater 19:711–716. doi:10.​1002/​adma.​200602098 CrossRef
Zurück zum Zitat De Wall SL, Painter C, Stone JD, Bandaranayake R, Wiley DC, Mitchison TJ, Stern LJ, Dedecker BS (2006) Noble metals strip peptides from class II MHC proteins. Nat Chem Biol 2:197–201. doi:10.1038/nchembio773 PubMedCrossRef De Wall SL, Painter C, Stone JD, Bandaranayake R, Wiley DC, Mitchison TJ, Stern LJ, Dedecker BS (2006) Noble metals strip peptides from class II MHC proteins. Nat Chem Biol 2:197–201. doi:10.​1038/​nchembio773 PubMedCrossRef
Zurück zum Zitat Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspension. Nat Phys Sci (Lond) 241:20–22ADS Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspension. Nat Phys Sci (Lond) 241:20–22ADS
Zurück zum Zitat Inoue K, Kurosumi K, Deng ZP (1982) An improvement of the device for rapid freezing by use of liquid propane and the application of immunocytochemistry to the resin section of rapid-frozen, substitution-fixed anterior pituitary gland. J Electron Microsc (Tokyo) 31:93–97 Inoue K, Kurosumi K, Deng ZP (1982) An improvement of the device for rapid freezing by use of liquid propane and the application of immunocytochemistry to the resin section of rapid-frozen, substitution-fixed anterior pituitary gland. J Electron Microsc (Tokyo) 31:93–97
Zurück zum Zitat Kienberger F, Stroh C, Kada G, Moser R, Baumgartner W, Pastushenko V, Rankl C, Schmidt U, Muller H, Orlova E, LeGrimellec C, Drenckhahn D, Blaas D, Hinterdorfer P (2003) Dynamic force microscopy imaging of native membranes. Ultramicroscopy 97:229–237. doi:10.1016/S0304-3991(03)00047-0 PubMedCrossRef Kienberger F, Stroh C, Kada G, Moser R, Baumgartner W, Pastushenko V, Rankl C, Schmidt U, Muller H, Orlova E, LeGrimellec C, Drenckhahn D, Blaas D, Hinterdorfer P (2003) Dynamic force microscopy imaging of native membranes. Ultramicroscopy 97:229–237. doi:10.​1016/​S0304-3991(03)00047-0 PubMedCrossRef
Zurück zum Zitat Li JY, Wang XM, Wang CX, Chen BA, Dai YY, Zhang RY, Song M, Lv G, Fu DG (2007) The enhancement effect of gold nanoparticles in drug delivery and as biomarkers of drug-resistant cancer cells. ChemMedChem 2:374–378. doi:10.1002/cmdc.200600264 PubMedCrossRef Li JY, Wang XM, Wang CX, Chen BA, Dai YY, Zhang RY, Song M, Lv G, Fu DG (2007) The enhancement effect of gold nanoparticles in drug delivery and as biomarkers of drug-resistant cancer cells. ChemMedChem 2:374–378. doi:10.​1002/​cmdc.​200600264 PubMedCrossRef
Zurück zum Zitat Liu YP, Meyer-Zaika W, Franzka S, Schmid G, Tsoli M, Kuhn H (2003) Gold-cluster degradation by the transition of B-DNA into A-DNA and the formation of nanowires. Angew Chem Int Ed 42:2853–2857. doi:10.1002/anie.200250235 CrossRef Liu YP, Meyer-Zaika W, Franzka S, Schmid G, Tsoli M, Kuhn H (2003) Gold-cluster degradation by the transition of B-DNA into A-DNA and the formation of nanowires. Angew Chem Int Ed 42:2853–2857. doi:10.​1002/​anie.​200250235 CrossRef
Zurück zum Zitat Murata F, Suzuki S, Tsuyama S, Suganuma T, Imada M, Furihata C (1985) Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach. Histochem J 17:967–980. doi:10.1007/BF01417946 PubMedCrossRef Murata F, Suzuki S, Tsuyama S, Suganuma T, Imada M, Furihata C (1985) Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach. Histochem J 17:967–980. doi:10.​1007/​BF01417946 PubMedCrossRef
Zurück zum Zitat Rhode S, Breuer A, Hesse J, Sonnleitner M, Pagler TA, Doringer M, Schutz GJ, Stangl H (2004) Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I. Cell Biochem Biophys 41:343–356. doi:10.1385/CBB:41:3:343 PubMedCrossRef Rhode S, Breuer A, Hesse J, Sonnleitner M, Pagler TA, Doringer M, Schutz GJ, Stangl H (2004) Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I. Cell Biochem Biophys 41:343–356. doi:10.​1385/​CBB:​41:​3:​343 PubMedCrossRef
Zurück zum Zitat Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR, Sastry M (2005) Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir 21:10644–10654. doi:10.1021/la0513712 PubMedCrossRef Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR, Sastry M (2005) Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir 21:10644–10654. doi:10.​1021/​la0513712 PubMedCrossRef
Zurück zum Zitat Tkachenko AG, Xie H, Liu YL, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL (2004) Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. Bioconjug Chem 15:482–490. doi:10.1021/bc034189q PubMedCrossRef Tkachenko AG, Xie H, Liu YL, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL (2004) Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. Bioconjug Chem 15:482–490. doi:10.​1021/​bc034189q PubMedCrossRef
Zurück zum Zitat Tseng CY, Ling EA, Wong WC (1984) A scanning and transmission electron microscopic study of amoeboid microglial cells in the prenatal rat brain following a maternal injection of 6-aminonicotinamide. J Anat 138:733–743PubMed Tseng CY, Ling EA, Wong WC (1984) A scanning and transmission electron microscopic study of amoeboid microglial cells in the prenatal rat brain following a maternal injection of 6-aminonicotinamide. J Anat 138:733–743PubMed
Zurück zum Zitat Yi H, Leunissen J, Shi G, Gutekunst C, Hersch S (2001) A novel procedure for pre-embedding double immunogold-silver labeling at the ultrastructural level. J Histochem Cytochem 49:279–284PubMed Yi H, Leunissen J, Shi G, Gutekunst C, Hersch S (2001) A novel procedure for pre-embedding double immunogold-silver labeling at the ultrastructural level. J Histochem Cytochem 49:279–284PubMed
Metadaten
Titel
Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels
verfasst von
Lan Yuan
Wei Wei
Juan Li
Zhiwei Sun
Hongfang Wang
Xiuzhi Zhang
Yueyue Chen
Publikationsdatum
01.07.2009
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 5/2009
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-009-9618-x

Weitere Artikel der Ausgabe 5/2009

Journal of Nanoparticle Research 5/2009 Zur Ausgabe

    Marktübersichten

    Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.