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Erschienen in: Clean Technologies and Environmental Policy 4/2012

01.08.2012 | Original Paper

Biological method for decolourisation of an azo dye: clean technology to reduce pollution load in dye waste water

verfasst von: J. Kanagaraj, T. Senthil Velan, A. B. Mandal

Erschienen in: Clean Technologies and Environmental Policy | Ausgabe 4/2012

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Abstract

A bacterium isolated and identified as Bacillus cereus from tannery effluent was employed for decolourisation of an azo dye. This strain rapidly decolourises the Acid Black dye sample that belongs to azo category. The optimum activity of B. cereus was found at pH 7.3, temperature 37°C, and duration of 4 days. The decolourisation rate was found out to be 80 and 96% for the agitated and static conditions. UV–vis spectra analysis showed that the peaks which appeared in the visible region of the treated dye disappeared, indicating complete decolourisation of dye. The FT-IR analysis for the B. cereus treated sample showed the transformation of azo linkage into N2 or NH3 or incorporated into complete biomass. The presence of aromatic amine in the decolourised sample indicated the presence of azoreductase activity. The mass spectra analysis showed the conversion of the azo dye into new intermediate metabolites such as p-nitroaniline, 2,8 diamino, 3,6 dithio 1-napthanol, and 2,8 diamino-1-napthalol with mol. wt. of 139, 240, and 174, respectively. COD and TOC values of the B. cereus treated sample showed reduction of 85 and 87%, respectively at 120 h duration.

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Metadaten
Titel
Biological method for decolourisation of an azo dye: clean technology to reduce pollution load in dye waste water
verfasst von
J. Kanagaraj
T. Senthil Velan
A. B. Mandal
Publikationsdatum
01.08.2012
Verlag
Springer-Verlag
Erschienen in
Clean Technologies and Environmental Policy / Ausgabe 4/2012
Print ISSN: 1618-954X
Elektronische ISSN: 1618-9558
DOI
https://doi.org/10.1007/s10098-011-0416-7

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