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24.10.2020 | Original Research

Production and characterization of bacterial cellulose scaffold and its modification with hyaluronic acid and gelatin for glioblastoma cell culture

verfasst von: Semra Unal, Sema Arslan, Betul Karademir Yilmaz, Faik Nuzhet Oktar, Ahmet Zeki Sengil, Oguzhan Gunduz

Erschienen in: Cellulose | Ausgabe 1/2021

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Abstract

Three-dimensional (3D) in vitro cell culture models have recently gained increasing interest in predicting the response of anticancer drugs. In this study first, we tried to obtain a novel hyaluronic acid (HA)/gelatin (Gel) modified bacterial cellulose (BC) composite scaffolds by in situ fermentation method. Morphological and chemical structures, wettability, and thermal stability of scaffolds were evaluated. In particular, the human glioblastoma (GBM) cancer cell line (U251) was seeded into BC/HA/Gel scaffolds to evaluate their potential as in vitro 3D cancer cell culture. MTT proliferation assay, scanning electron microscopy, and confocal microscopy were utilised to determine cell proliferation, morphology and adhesion. The results suggest that our hyaluronic acid and gelatin modified bacterial cellulose scaffold is promising to be used as in vitro 3D culture of GBM cells and may be used to predict treatment response or reactions of new therapeutics.

Vorheriger Artikel Sustainable process for the production of cellulose by an Acetobacter pasteurianus RSV-4 (MTCC 25117) on whey medium

Nächster Artikel Furfural and levoglucosenone production from the pyrolysis of ionic liquid pre-treated sugarcane straw

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Titel: Production and characterization of bacterial cellulose scaffold and its modification with hyaluronic acid and gelatin for glioblastoma cell culture
verfasst von: Semra Unal
Sema Arslan
Betul Karademir Yilmaz
Faik Nuzhet Oktar
Ahmet Zeki Sengil
Oguzhan Gunduz
Publikationsdatum: 24.10.2020
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 1/2021
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-020-03528-5

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