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Biomass Conversion and Biorefinery

05.01.2023 | Original Article

Optimization of sequential alkali/acid pretreatment of corn cob for xylitol production by Debaryomyces nepalensis

verfasst von: Yogeswar Mohanasundaram, Vishnu Damodaran Nambissan, Sathyanarayana N. Gummadi

Erschienen in: Biomass Conversion and Biorefinery

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Abstract

A two-step sequential dilute alkali/acid pretreatment process was developed to improve the breakdown of the lignocellulosic structure of corn cob. Uniform design was used to optimize the hydrolysis process and multiple regression models were developed. Alkali hydrolysis of corn cob at optimum conditions of 1.81% (w/v) NaOH concentration and 5 (w/v) solid:liquid ratio for 90 min resulted in 82.03% lignin removal. The biomass on further dilute acid pretreatment at the optimized conditions of 6% (w/v) H₂SO₄ and 5 w/v solid:liquid ratio for 15 min resulted in 74% xylose yield. The xylose-rich acid hydrolysate was used as a substrate to produce xylitol by Debaryomyces nepalensis NCYC 3413. It resulted in a xylitol yield of 0.467 g/g of xylose and a xylitol titer of 21.15 g/L. The changes in the physical and chemical structure of the corn cob due to the sequential alkali/acid pretreatment were analyzed by SEM, XRD, FTIR, and TGA.

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Dhar KS, Wendisch VF, Nampoothiri KM (2016) Engineering of Corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars. J Biotechnol 230:63–71. https://doi.org/10.1016/j.jbiotec.2016.05.011CrossRef

Gardner E (2017) Alternative sugars: xylitol. Br Dent J 223(3):141. https://doi.org/10.1038/sj.bdj.2017.650CrossRef

Narisetty V et al (2021) High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits hydrolysates. Bioresour Technol 342:126005. https://doi.org/10.1016/j.biortech.2021.126005CrossRef

Dasgupta D, Bandhu S, Adhikari DK, Ghosh D (2017) Challenges and prospects of xylitol production with whole cell bio-catalysis: a review. Microbiol Res 197:9–21. https://doi.org/10.1016/j.micres.2016.12.012CrossRef

Zhang D, Ong YL, Li Z, Wu JC (2012) Optimization of dilute acid-catalyzed hydrolysis of oil palm empty fruit bunch for high yield production of xylose. Chem Eng J 181–182:636–642. https://doi.org/10.1016/j.cej.2011.12.030CrossRef

Agbor VB, Cicek N, Sparling R, Berlin A, Levin DB (2011) Biomass pretreatment: fundamentals toward application. Biotechnol Adv 29(6):675–685. https://doi.org/10.1016/j.biotechadv.2011.05.005CrossRef

Yang M, Lan M, Gao X, Dou Y, Zhang X (2021) Sequential dilute acid/alkali pretreatment of corncobs for ethanol production. Energy Source A Recov Util Environ Effects 43(14):1769–1778. https://doi.org/10.1080/15567036.2019.1648596CrossRef

Kim S, Park JM, Seo J-W, Kim CH (2012) Sequential acid-/alkali-pretreatment of empty palm fruit bunch fiber. Bioresour Technol 109:229–233. https://doi.org/10.1016/j.biortech.2012.01.036CrossRef

Shi F, Wang Y, Davaritouchaee M, Yao Y, Kang K (2020) Directional structure modification of poplar biomass-inspired high efficacy of enzymatic hydrolysis by sequential dilute acid–alkali treatment. ACS Omega 5(38):24780–24789. https://doi.org/10.1021/acsomega.0c03419CrossRef

10.

Hemansi, Gupta R, Aswal VK, Saini JK (2020) Sequential dilute acid and alkali deconstruction of sugarcane bagasse for improved hydrolysis: insight from small angle neutron scattering (SANS). Renew Energy 147:2091–2101. https://doi.org/10.1016/j.renene.2019.10.003CrossRef

11.

Lee JW, Kim JY, Jang HM, Lee MW, Park JM (2015) Sequential dilute acid and alkali pretreatment of corn stover: sugar recovery efficiency and structural characterization. Bioresour Technol 182:296–301. https://doi.org/10.1016/j.biortech.2015.01.116CrossRef

12.

Fang K-T, Lin DKJ, Winker P, Zhang Y (2000) Uniform design: theory and application. Technometrics 42(3):237–248. https://doi.org/10.1080/00401706.2000.10486045MathSciNetCrossRefMATH

13.

Pappu JSM, Gummadi SN (2016) Multi response optimization for enhanced xylitol production by Debaryomyces nepalensis in bioreactor. 3 Biotech 6(2):151. https://doi.org/10.1007/s13205-016-0467-xCrossRef

14.

Paidimuddala B, Gummadi SN (2014) Bioconversion of non-detoxified hemicellulose hydrolysates to xylitol by halotolerant yeast Debaryomyces nepalensis NCYC 3413. J Microb Biochem Technol 06(06). https://doi.org/10.4172/1948-5948.1000163

15.

Si S et al (2015) Lignin extraction distinctively enhances biomass enzymatic saccharification in hemicelluloses-rich Miscanthus species under various alkali and acid pretreatments. Bioresour Technol 183:248–254. https://doi.org/10.1016/j.biortech.2015.02.031CrossRef

16.

J. M. Wells, E. Drielak, K. C. Surendra, and S. Kumar Khanal, “Hot water pretreatment of lignocellulosic biomass: modeling the effects of temperature, enzyme and biomass loadings on sugar yield,” Bioresour Technol, vol. 300, p. 122593, 2020, doi: https://doi.org/10.1016/j.biortech.2019.122593.

17.

Atanasova M, Yordanova G, Nenkova R, Ivanov Y, Godjevargova T, Dinev D (2019) Brewing yeast viability measured using a novel fluorescent dye and image cytometer. Biotechnol Biotechnol Equip 33(1):548–558. https://doi.org/10.1080/13102818.2019.1593053CrossRef

18.

Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29(10):786–794. https://doi.org/10.1177/004051755902901003CrossRef

19.

Su Y et al (2015) Fractional pretreatment of lignocellulose by alkaline hydrogen peroxide: characterization of its major components. Food Bioprod Process 94:322–330. https://doi.org/10.1016/j.fbp.2014.04.001CrossRef

20.

Yasuda S, Fukushima K, Kakehi A (2001) Formation and chemical structures of acid-soluble lignin I: sulfuric acid treatment time and acid-soluble lignin content of hardwood. J Wood Sci 47(1):69–72. https://doi.org/10.1007/BF00776648CrossRef

21.

Moodley P, Gueguim kana EB (2017) Development of a steam or microwave-assisted sequential salt-alkali pretreatment for lignocellulosic waste: effect on delignification and enzymatic hydrolysis. Energy Convers Manag 148:801–808. https://doi.org/10.1016/j.enconman.2017.06.056CrossRef

22.

Gabhane J, William SPMP, Vaidya AN, Das S, Wate SR (2015) Solar assisted alkali pretreatment of garden biomass: effects on lignocellulose degradation, enzymatic hydrolysis, crystallinity and ultra-structural changes in lignocellulose. Waste Manag 40:92–99. https://doi.org/10.1016/j.wasman.2015.03.002CrossRef

23.

24.

Kaur A, Kuhad RC (2019) Valorization of rice straw for ethanol production and lignin recovery using combined acid-alkali pre-treatment. Bioenergy Res 12(3):570–582. https://doi.org/10.1007/s12155-019-09988-3CrossRef

25.

Jung YH, Kim IJ, Kim HK, Kim KH (2013) Dilute acid pretreatment of lignocellulose for whole slurry ethanol fermentation. Bioresour Technol 132:109–114. https://doi.org/10.1016/j.biortech.2012.12.151CrossRef

26.

Marzialetti T, Valenzuela Olarte MB, Sievers C, Hoskins TJC, Agrawal PK, Jones CW (2008) Dilute acid hydrolysis of loblolly pine: a comprehensive approach. Ind Eng Chem Res 47(19):7131–7140. https://doi.org/10.1021/ie800455fCrossRef

27.

Sun Y, Lin LU (2010) Hydrolysis behavior of bamboo fiber in formic acid reaction system. J Agric Food Chem 58(4):2253–2259. https://doi.org/10.1021/jf903731sCrossRef

28.

Jiang L et al (2017) Comprehensive utilization of hemicellulose and cellulose to release fermentable sugars from corncobs via acid hydrolysis and fast pyrolysis. ACS Sustain Chem Eng 5(6):5208–5213. https://doi.org/10.1021/acssuschemeng.7b00561CrossRef

29.

Rafiqul ISM, Sakinah AMM, Karim MR (2014) Production of xylose from Meranti wood sawdust by dilute acid hydrolysis. Appl Biochem Biotechnol 174(2):542–555. https://doi.org/10.1007/s12010-014-1059-zCrossRef

30.

Karuna N et al (2014) The impact of alkali pretreatment and post-pretreatment conditioning on the surface properties of rice straw affecting cellulose accessibility to cellulases. Bioresour Technol 167:232–240. https://doi.org/10.1016/j.biortech.2014.05.122CrossRef

31.

Li M, Jia Z, Wan G, Wang S, Min D (2020) Enhancing isolation of p-coumaric and ferulic acids from sugarcane bagasse by sequential hydrolysis. Chem Pap 74(2):499–507. https://doi.org/10.1007/s11696-019-00890-yCrossRef

32.

Argun H, Onaran G (2016) Glucose and 5-hydroxymethylfurfural production from cellulosic waste by sequential alkaline and acid hydrolysis. Renew Energy 96:442–449. https://doi.org/10.1016/j.renene.2016.04.082CrossRef

33.

Qin F, Johansen AZ, Mussatto SI (2018) Evaluation of different pretreatment strategies for protein extraction from brewer’s spent grains. Ind Crops Prod 125:443–453. https://doi.org/10.1016/j.indcrop.2018.09.017CrossRef

34.

Sewsynker-Sukai Y, Suinyuy TN, Gueguim Kana EB (2018) Development of a sequential alkalic salt and dilute acid pretreatment for enhanced sugar recovery from corn cobs. Energy Convers Manag 160:22–30. https://doi.org/10.1016/j.enconman.2018.01.024CrossRef

35.

Sanchez A et al (2015) Sequential pretreatment strategies under mild conditions for efficient enzymatic hydrolysis of wheat straw. Bioprocess Biosyst Eng 38(6):1127–1141. https://doi.org/10.1007/s00449-015-1355-1CrossRef

36.

Hoşgün EZ, Biran Ay S, Bozan B (2021) Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells. Prep Biochem Biotechnol 51(6):570–579. https://doi.org/10.1080/10826068.2020.1836657CrossRef

37.

Mohapatra S, Pattathil S, Thatoi H (2017) Structural and functional characterization of two Pennisetum sp. biomass during ultrasono-assisted alkali pretreatment and enzymatic hydrolysis for understanding the mechanism of targeted delignification and enhanced saccharification. ACS Sustain Chem Eng 5(8):6486–6497. https://doi.org/10.1021/acssuschemeng.7b00596CrossRef

38.

Karp EM et al (2014) Alkaline pretreatment of corn stover: bench-scale fractionation and stream characterization. ACS Sustain Chem Eng 2(6):1481–1491. https://doi.org/10.1021/sc500126uCrossRef

39.

Zhu T, Li P, Wang X, Yang W, Chang H, Ma S (2014) Optimization of formic acid hydrolysis of corn cob in xylose production. Korean J Chem Eng 31(9):1624–1631. https://doi.org/10.1007/s11814-014-0073-8CrossRef

40.

Frederick N et al (2014) Poplar ( Populus deltoides L.): the effect of washing pretreated biomass on enzymatic hydrolysis and fermentation to ethanol. ACS Sustain Chem Eng 2(7):1835–1842. https://doi.org/10.1021/sc500188sCrossRef

41.

Xu Q-Q et al (2017) Enhancing enzymatic hydrolysis of corn cob, corn stover and sorghum stalk by dilute aqueous ammonia combined with ultrasonic pretreatment. Ind Crops Prod 109:220–226. https://doi.org/10.1016/j.indcrop.2017.08.038CrossRef

42.

Liu Z-H, Qin L, Li B-Z, Yuan Y-J (2015) Physical and chemical characterizations of corn stover from leading pretreatment methods and effects on enzymatic hydrolysis. ACS Sustain Chem Eng 3(1):140–146. https://doi.org/10.1021/sc500637cCrossRef

43.

Thangavelu K, Desikan R, Taran OP, Uthandi S (2018) Delignification of corncob via combined hydrodynamic cavitation and enzymatic pretreatment: process optimization by response surface methodology. Biotechnol Biofuels 11(1). https://doi.org/10.1186/s13068-018-1204-y

44.

Li M-F, Yu P, Li S-X, Wu X-F, Xiao X, Bian J (2017) Sequential two-step fractionation of lignocellulose with formic acid organosolv followed by alkaline hydrogen peroxide under mild conditions to prepare easily saccharified cellulose and value-added lignin. Energy Convers Manag 148:1426–1437. https://doi.org/10.1016/j.enconman.2017.07.008CrossRef

45.

Yang C et al (2020) Ball milling promoted direct liquefaction of lignocellulosic biomass in supercritical ethanol. Front Chem Sci Eng 14(4):605–613. https://doi.org/10.1007/s11705-019-1841-0CrossRef

46.

Cheng K-K, Wu J, Lin Z-N, Zhang J-A (2014) Aerobic and sequential anaerobic fermentation to produce xylitol and ethanol using non-detoxified acid pretreated corncob. Biotechnol Biofuels 7(1):166. https://doi.org/10.1186/s13068-014-0166-yCrossRef

47.

Oktaviani M, Mangunwardoyo W, Hermiati E (2021) Characteristics of adapted and non-adapted Candida tropicalis InaCC Y799 during fermentation of detoxified and undetoxified hemicellulosic hydrolysate from sugarcane trash for xylitol production. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-021-02087-4CrossRef

48.

Ping Y, Ling H-Z, Song G, Ge J-P (2013) Xylitol production from non-detoxified corncob hemicellulose acid hydrolysate by Candida tropicalis. Biochem Eng J 75:86–91. https://doi.org/10.1016/j.bej.2013.03.022CrossRef

49.

Goli JK, Hameeda B (2021) Production of xylitol and ethanol from acid and enzymatic hydrolysates of Typha latifolia by Candida tropicalis JFH5 and Saccharomyces cerevisiae VS3. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-021-01868-1CrossRef

50.

Du C, Li Y, Zong H, Yuan T, Yuan W, Jiang Y (2020) Production of bioethanol and xylitol from non-detoxified corn cob via a two-stage fermentation strategy. Bioresour Technol 310:123427. https://doi.org/10.1016/j.biortech.2020.123427CrossRef

51.

Baptista SL, Carvalho LC, Romaní A, Domingues L (2020) Development of a sustainable bioprocess based on green technologies for xylitol production from corn cob. Ind Crops Prod 156:112867. https://doi.org/10.1016/j.indcrop.2020.112867CrossRef

52.

Kamat S, Gaikwad S, Ravi Kumar A, Gade WN (2013) Xylitol production by Cyberlindnera ( Williopsis) saturnus, a tropical mangrove yeast from xylose and corn cob hydrolysate. J Appl Microbiol 115(6):1357–1367. https://doi.org/10.1111/jam.12327CrossRef

53.

Kumar V, Sandhu PP, Ahluwalia V, Mishra BB, Yadav SK (2019) Improved upstream processing for detoxification and recovery of xylitol produced from corncob. Bioresour Technol 291:121931. https://doi.org/10.1016/j.biortech.2019.121931CrossRef

Titel: Optimization of sequential alkali/acid pretreatment of corn cob for xylitol production by Debaryomyces nepalensis
verfasst von: Yogeswar Mohanasundaram
Vishnu Damodaran Nambissan
Sathyanarayana N. Gummadi
Publikationsdatum: 05.01.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Biomass Conversion and Biorefinery
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-022-03660-1