nach oben

Cellulose

Erschienen in:

26.10.2020 | Original Research

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

verfasst von: Li Huang, Wei Yuan, Yue Hong, Suna Fan, Xiang Yao, Tao Ren, Lujie Song, Gesheng Yang, Yaopeng Zhang

Erschienen in: Cellulose | Ausgabe 1/2021

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

A novel biomaterial ink consisting of regenerated silk fibroin (SF) and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose (OBC) nanofibrils was developed for 3D printing lung tissue scaffold. Silk fibroin backbones were cross-linked using horseradish peroxide/H₂O₂ to form printed hydrogel scaffolds. OBC with a concentration of 7wt% increased the viscosity of inks during the printing process and further improved the shape fidelity of the scaffolds. Rheological measurements and image analyses were performed to evaluate inks printability and print shape fidelity. Three-dimensional construct with ten layers could be printed with ink of 1SF-2OBC (SF/OBC = 1/2, w/w). The composite hydrogel of 1SF-1OBC (SF/OBC = 1/1, w/w) printed at 25 °C exhibited a significantly improved compressive strength of 267 ± 13 kPa and a compressive stiffness of 325 ± 14 kPa at 30% strain, respectively. The optimized printing parameters for 1SF-1OBC were 0.3 bar of printing pressure, 45 mm/s of printing speed and 410 μm of nozzle diameter. Furthermore, OBC nanofibrils could be induced to align along the print lines over 60% degree of orientation, which were analyzed by SEM and X-ray diffraction. The orientation of OBC nanofibrils along print lines provided physical cues for guiding the orientation of lung epithelial stem cells, which maintained the ability to proliferate and kept epithelial phenotype after 7 days’ culture. The 3D printed SF-OBC scaffolds are promising for applications in lung tissue engineering.

Vorheriger Artikel Cellulose aerogel particles: control of particle and textural properties in jet cutting process

Nächster Artikel Effects of hemicellulose composition and content on the interaction between cellulose nanofibers

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Nur mit Berechtigung zugänglich

Abouzeid RE, Khiari R, Beneventi D, Dufresne A (2018) Biomimetic mineralization of three-dimensional printed alginate/tempo-oxidized cellulose nanofibril scaffolds for bone tissue engineering. Biomacromol 19:4442–4452. https://doi.org/10.1021/acs.biomac.8b01325CrossRef

Aljohani W, Ullah MW, Zhang XL, Yang G (2018) Bioprinting and its applications in tissue engineering and regenerative medicine. Int J Biol Macromol 107:261–275. https://doi.org/10.1016/j.ijbiomac.2017.08.171CrossRefPubMed

Campodoni E et al (2020) Blending gelatin and cellulose nanofibrils: biocomposites with tunable degradability and mechanical behavior. Nanomaterials 10:1219. https://doi.org/10.3390/nano10061219CrossRefPubMedCentral

Chawla S, Midha S, Sharma A, Ghosh S (2018) Silk-based bioinks for 3d bioprinting. Adv Healthc Mater 7:1701204. https://doi.org/10.1002/adhm.201701204CrossRef

Chen J, Zhuang A, Shao HL, Hu XC, Zhang YP (2017) Robust silk fibroin/bacterial cellulose nanoribbon composite scaffolds with radial lamellae and intercalation structure for bone regeneration. J Mater Chem B 5:3640–3650. https://doi.org/10.1039/c7tb00485kCrossRefPubMed

Cheng F et al (2019) Generation of cost-effective paper-based tissue models through matrix-assisted sacrificial 3d printing. Nano Lett 19:3603–3611. https://doi.org/10.1021/acs.nanolett.9b00583CrossRefPubMedPubMedCentral

Chinga-Carrasco G (2018) Potential and limitations of nanocelluloses as components in biocomposite inks for three-dimensional bioprinting and for biomedical devices. Biomacromol 19:701–711. https://doi.org/10.1021/acs.biomac.8b00053CrossRef

Chirila TV, Suzuki S, Papolla C (2017) A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking. Biotechnol Appl Bioc 64:771–781. https://doi.org/10.1002/bab.1552CrossRef

Comber EM, Palchesko RN, Ng WH, Ren X, Cook KE (2019) De novo lung biofabrication: clinical need, construction methods, and design strategy. Transl Res 211:1–18. https://doi.org/10.1016/j.trsl.2019.04.008CrossRefPubMed

Dai L et al (2019) 3D printing using plant-derived cellulose and its derivatives: a review. Carbohyd Polym 203:71–86. https://doi.org/10.1016/j.carbpol.2018.09.027CrossRef

Das S et al (2015) Bioprintable, cell-laden silk fibroin-gelatin hydrogel supporting multilineage differentiation of stem cells for fabrication of three-dimensional tissue constructs. Acta Biomater 11:233–246CrossRef

Das S, Pati F, Chameettachal S, Pahwa S, Ray AR, Dhara S, Ghosh S (2013) Enhanced redifferentiation of chondrocytes on microperiodic silk/gelatin scaffolds: toward tailor-made tissue engineering. Biomacromol 14:311–321. https://doi.org/10.1021/bm301193tCrossRef

DeSimone E, Schacht K, Jungst T, Groll J, Scheibel T (2015) Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks. Pure Appl Chem 87:737–749. https://doi.org/10.1515/pac-2015-0106CrossRef

Dorishetty P et al (2020) Tunable biomimetic hydrogels from silk fibroin and nanocellulose. ACS Sustain Chem Eng 8:2375–2389. https://doi.org/10.1021/acssuschemeng.9b05317CrossRef

El-Hashash AH, Warburton D (2011) Cell polarity and spindle orientation in the distal epithelium of embryonic lung. Dev Dynam 240:441–445. https://doi.org/10.1002/dvdy.22551CrossRef

Fernandes EM, Pires RA, Mano JF, Reis RL (2013) Bionanocomposites from lignocellulosic resources: properties, applications and future trends for their use in the biomedical field. Prog Polym Sci 38:1415–1441. https://doi.org/10.1016/j.progpolymsci.2013.05.013CrossRef

French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896. https://doi.org/10.1007/s10570-013-0030-4CrossRef

Ghaedi M, Mendez JJ, Bove PF, Sivarapatna A, Raredon MSB, Niklason LE (2014) Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactor. Biomaterials 35:699–710. https://doi.org/10.1016/j.biomaterials.2013.10.018CrossRefPubMed

Ghosh S, Parker ST, Wang XY, Kaplan DL, Lewis JA (2008) Direct-write assembly of microperiodic silk fibroin scaffolds for tissue engineering applications. Adv Funct Mater 18:1883–1889CrossRef

Hu X, Shmelev K, Sun L, Gil ES, Park SH, Cebe P, Kaplan DL (2011) regulation of silk material structure by temperature-controlled water vapor annealing. Biomacromol 12:1686–1696CrossRef

Huang L et al (2019a) Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores. Carbohyd Polym 221:146–156. https://doi.org/10.1016/j.carbpol.2019.05.080CrossRef

Huang L et al (2019b) Silk scaffolds with gradient pore structure and improved cell infiltration performance. Mat Sci Eng C-Mater 94:179–189. https://doi.org/10.1016/j.msec.2018.09.034CrossRef

Huang W, Xiao Y, Shi X (2019c) Construction of electrospun organic/inorganic hybrid nanofibers for drug delivery and tissue engineering applications. Adv Fiber Mater 1:32–45. https://doi.org/10.1007/s42765-019-00007-wCrossRef

Jose RR, Brown JE, Polido KE, Omenetto FG, Kaplan DL (2015) Polyol-silk bioink formulations as two-part room-temperature curable materials for 3d printing. ACS Biomater Sci Eng 1:780–788CrossRef

Jungst T, Smolan W, Schacht K, Scheibel T, Groll J (2016) Strategies and molecular design criteria for 3D printable hydrogels. Chem Rev 116:1496–1539. https://doi.org/10.1021/acs.chemrev.5b00303CrossRefPubMed

Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A (2016) A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol 34:312–319. https://doi.org/10.1038/nbt.3413CrossRefPubMed

Kim DH, Provenzano PP, Smith CL, Levchenko A (2012) Matrix nanotopography as a regulator of cell function. J Cell Biol 197:351–360. https://doi.org/10.1083/jcb.201108062CrossRefPubMedPubMedCentral

Kurisawa M, Lee F, Wang LS, Chung JE (2010) Injectable enzymatically crosslinked hydrogel system with independent tuning of mechanical strength and gelation rate for drug delivery and tissue engineering. J Mater Chem 20:5371–5375. https://doi.org/10.1039/b926456fCrossRef

Kyle S, Jessop ZM, Al-Sabah A, Whitaker IS (2017) “Printability” of candidate biomaterials for extrusion based 3d printing: state-of-the-art. Adv Healthc Mater 6:1700264. https://doi.org/10.1002/adhm.201700264CrossRef

Lai C, Zhang SJ, Chen XC, Sheng LY (2014) Nanocomposite films based on TEMPO-mediated oxidized bacterial cellulose and chitosan. Cellulose 21:2757–2772. https://doi.org/10.1007/s10570-014-0330-3CrossRef

Leisk GG, Lo TJ, Yucel T, Lu Q, Kaplan DL (2010) Electrogelation for protein adhesives. Adv Mater 22:711–715. https://doi.org/10.1002/adma.200902643CrossRefPubMed

Lin YM, Boccaccini AR, Polak JM, Bishop AE (2006) Biocompatibility of poly-DL-lactic acid (PDLLA) for lung tissue engineering. J Biomater Appl 21:109–118. https://doi.org/10.1177/0885328206057952CrossRefPubMed

Liu J, Sun LS, Xu WY, Wang QQ, Yu SJ, Sun JZ (2019) Current advances and future perspectives of 3D printing natural-derived biopolymers. Carbohyd Polym 207:297–316. https://doi.org/10.1016/j.carbpol.2018.11.077CrossRef

Malda J et al (2013) 25th anniversary article: engineering hydrogels for biofabrication. Adv Mater 25:5011–5028. https://doi.org/10.1002/adma.201302042CrossRefPubMed

Markstedt K, Mantas A, Tournier I, Avila HM, Hagg D, Gatenholm P (2015) 3D bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications. Biomacromol 16:1489–1496. https://doi.org/10.1021/acs.biomac.5b00188CrossRef

McCarthy RR, Ullah MW, Booth P, Pei E, Yang G (2019) The use of bacterial polysaccharides in bioprinting. Biotechnol Adv. https://doi.org/10.1016/j.biotechadv.2019.107448CrossRefPubMed

Miller AJ, Dye BR, Ferrer-Torres D, Hill DR, Overeem AW, Shea LD, Spence JR (2019) Generation of lung organoids from human pluripotent stem cells in vitro. Nat Protoc 14:518–540. https://doi.org/10.1038/s41596-018-0104-8CrossRefPubMedPubMedCentral

Mita K, Ichimura S, Zama M, James TC (1988) Specific codon usage pattern and its implications on the secondary structure of silk fibroin messenger-rna. J Mol Biol 203:917–925. https://doi.org/10.1016/0022-2836(88)90117-9CrossRefPubMed

Mondrinos MJ, Koutzaki S, Jiwanmall E, Li MY, Dechadarevian JP, Lelkes PI, Finck CM (2006) Engineering three-dimensional pulmonary tissue constructs. Tissue Eng 12:717–728. https://doi.org/10.1089/ten.2006.12.717CrossRefPubMed

Moniri M et al (2017) Production and status of bacterial cellulose in biomedical engineering. Nanomaterials-Basel 7:257. https://doi.org/10.3390/nano7090257CrossRefPubMedCentral

Moohan J et al (2020) Cellulose nanofibers and other biopolymers for biomedical applications. Rev Appl Sci-Basel 10:65. https://doi.org/10.3390/app10010065CrossRef

Moon RJ, Martini A, Nairn J, Simonsen J, Youngblood J (2011) Cellulose nanomaterials review: structure, properties and nanocomposites. Chem Soc Rev 40:3941–3994. https://doi.org/10.1039/c0cs00108bCrossRefPubMed

Murphy AR, Kaplan DL (2009) Biomedical applications of chemically-modified silk fibroin. J Mater Chem 19:6443–6450. https://doi.org/10.1039/b905802hCrossRefPubMedPubMedCentral

Nimeskern L, Avila HM, Sundberg J, Gatenholm P, Muller R, Stok KS (2013) Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement. J Mech Behav Biomed 22:12–21. https://doi.org/10.1016/j.jmbbm.2013.03.005CrossRef

Ojansivu M et al (2019) Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells. Biofabrication. https://doi.org/10.1088/1758-5090/ab0692/metaCrossRefPubMed

Oliveira Barud HG et al (2015) Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydr Polym 128:41–51. https://doi.org/10.1016/j.carbpol.2015.04.007CrossRefPubMed

Ouyang L, Yao R, Zhao Y, Sun W (2016) Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells. Biofabrication 8:035020. https://doi.org/10.1088/1758-5090/8/3/035020CrossRefPubMed

Partlow BP et al (2014) Highly tunable elastomeric silk biomaterials. Adv Funct Mater 24:4615–4624. https://doi.org/10.1002/adfm.201400526CrossRefPubMedPubMedCentral

Pereira RFP, Silva MM, Bermudez VD (2015) Bombyx mori silk fibers: an outstanding family of materials. Macromol Mater Eng 300:1171–1198. https://doi.org/10.1002/mame.201400276CrossRef

Plosa EJ, Gooding KA, Zent R, Prince LS (2012) Nonmuscle myosin II regulation of lung epithelial morphology. Dev Dynam 241:1770–1781. https://doi.org/10.1002/dvdy.23866CrossRef

Poon JCH et al (2018) Design of biomimetic substrates for long-term maintenance of alveolar epithelial cells. Biomater Sci-Uk 6:292–303. https://doi.org/10.1039/c7bm00647kCrossRef

Prince E, Kumacheva E (2019) Design and applications of man-made biomimetic fibrillar hydrogels. Nat Rev Mater 4:99–115. https://doi.org/10.1038/s41578-018-0077-9CrossRef

Rashad A et al (2019) Inflammatory responses and tissue reactions to wood-Based nanocellulose scaffolds. Mat Sci Eng C-Mater 97:208–221. https://doi.org/10.1016/j.msec.2018.11.068CrossRef

Schwarz S, Avila HM, Rotter N, Gatenholm P (2015) 3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for patient-specific auricular cartilage regeneration. Tissue Eng Pt A 21:S373–S373CrossRef

Siqueira G et al (2017) Cellulose nanocrystal inks for 3D printing of textured cellular architectures. Adv Funct Mater 27:1604619. https://doi.org/10.1002/adfm.201604619CrossRef

Song WG, Liu DG, Prempeh N, Song RJ (2017) Fiber alignment and liquid crystal orientation of cellulose nanocrystals in the electrospun nanofibrous mats. Biomacromol 18:3273–3279. https://doi.org/10.1021/acs.biomac.7b00927CrossRef

Stalling SS, Akintoye SO, Nicoll SB (2009) Development of photocrosslinked methylcellulose hydrogels for soft tissue reconstruction. Acta Biomater 5:1911–1918. https://doi.org/10.1016/j.actbio.2009.02.020CrossRefPubMed

Su DH, Yao M, Liu J, Zhong YM, Chen X, Shao ZZ (2017) Enhancing mechanical properties of silk fibroin hydrogel through restricting the growth of beta-sheet domains. ACS Appl Mater Inter 9:17490–17499. https://doi.org/10.1021/acsami.7b04623CrossRef

Sultan S, Mathew AP (2018) 3D printed scaffolds with gradient porosity based on a cellulose nanocrystal hydrogel. Nanoscale 10:4421–4431. https://doi.org/10.1039/c7nr08966jCrossRefPubMed

Sun L, Parker ST, Syoji D, Wang XL, Lewis JA, Kaplan DL (2012) Direct-write assembly of 3d silk/hydroxyapatite scaffolds for bone co-cultures. Adv Healthc Mater 1:729–735. https://doi.org/10.1002/adhm.201200057CrossRefPubMedPubMedCentral

Tebyanian H, Karami A, Nourani MR, Motavallian E, Barkhordari A, Yazdanian M, Seifalian A (2019) Lung tissue engineering: an update. J Cell Physiol 234:19256–19270. https://doi.org/10.1002/jcp.28558CrossRefPubMed

Terry AE, Knight DP, Porter D, Vollrath F (2004) PH induced changes in the rheology of silk fibroin solution from the middle division of Bombyx mori silkworm. Biomacromol 5:768–772CrossRef

Theocharis AD, Skandalis SS, Gialeli C, Karamanos NK (2016) Extracellular matrix structure. Adv Drug Deliver Rev 97:4–27. https://doi.org/10.1016/j.addr.2015.11.001CrossRef

Wagner DE et al (2014) Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration. Biomaterials 35:2664–2679. https://doi.org/10.1016/j.biomaterials.2013.11.078CrossRefPubMedPubMedCentral

Wang BX et al (2018) Use of heparinized bacterial cellulose based scaffold for improving angiogenesis in tissue regeneration. Carbohyd Polym 181:948–956. https://doi.org/10.1016/j.carbpol.2017.11.055CrossRef

Wang K, Ma Q, Zhang Y-M, Han G-T, Qu C-X, Wang S-D (2019) Preparation of bacterial cellulose/silk fibroin double-network hydrogel with high mechanical strength and biocompatibility for artificial cartilage. Cellulose. https://doi.org/10.1007/s10570-019-02869-0CrossRefPubMed

Wang BX et al (2020) Urethra-inspired biomimetic scaffold: a therapeutic strategy to promote angiogenesis for urethral regeneration in a rabbit model. Acta Biomater 102:247–258. https://doi.org/10.1016/j.actbio.2019.11.026CrossRefPubMed

Wlodarczyk-Biegun MK, del Campo A (2017) 3D bioprinting of structural proteins. Biomaterials 134:180–201CrossRef

Wu YD, He JM, Cheng WL, Gu HB, Guo ZH, Gao S, Huang YD (2012) Oxidized regenerated cellulose-based hemostat with microscopically gradient structure. Carbohyd Polym 88:1023–1032. https://doi.org/10.1016/j.carbpol.2012.01.058CrossRef

Xu WY, Wang XJ, Sandler N, Willfor S, Xu CL (2018) Three-dimensional printing of wood-derived biopolymers: a review focused on biomedical applications. ACS Sustain Chem Eng 6:5663–5680. https://doi.org/10.1021/acssuschemeng.7b03924CrossRefPubMedPubMedCentral

Yan LP et al (2016) Tumor growth suppression induced by biomimetic silk fibroin hydrogels. Sci Rep-UK 6:31037. https://doi.org/10.1038/srep31037CrossRef

Yao JJ, Chen SY, Chen Y, Wang BX, Pei QB, Wang HP (2017) Macrofibers with high mechanical performance based on aligned bacterial cellulose nanofibers. ACS Appl Mater Inter 9:20330–20339. https://doi.org/10.1021/acsami.6b14650CrossRef

Zheng ZZ et al (2018) 3D bioprinting of self-standing silk-based bioink. Adv Healthc Mater 7:1701026. https://doi.org/10.1002/adhm.201701026CrossRef

Titel: 3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells
verfasst von: Li Huang
Wei Yuan
Yue Hong
Suna Fan
Xiang Yao
Tao Ren
Lujie Song
Gesheng Yang
Yaopeng Zhang
Publikationsdatum: 26.10.2020
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 1/2021
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-020-03526-7

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Weitere Artikel der Ausgabe 1/2021

Facile fabrication of robust and healable superhydrophobic cotton fabric with flower-like Ni(OH)2@ODA micro-nanoparticles

Power transformer cellulosic insulation destruction assessment using a calculated index composed of CO, CO2, 2-Furfural, and Acetylene

Exponential decay analysis: a flexible, robust, data-driven methodology for analyzing sorption kinetic data

Cellulose phenylcarbamate-derived hybrid bead-type chiral packing materials for efficient chiral recognition

Cholesterol removal via cyclodextrin-decoration on cellulose nanocrystal (CNC)-grafted poly(HEMA-GMA) nanocomposite adsorbent

High-performance homogenized and spray coated nanofibrillated cellulose-montmorillonite barriers