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Medical & Biological Engineering & Computing
Erschienen in: Medical & Biological Engineering & Computing 10/2010

01.10.2010 | Special Issue - Original Article

Micro/nano-fabrication technologies for cell biology

verfasst von: Tongcheng Qian, Yingxiao Wang

Erschienen in: Medical & Biological Engineering & Computing | Ausgabe 10/2010

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Abstract

Micro/nano-fabrication techniques, such as soft lithography and electrospinning, have been well-developed and widely applied in many research fields in the past decade. Due to the low costs and simple procedures, these techniques have become important and popular for biological studies. In this review, we focus on the studies integrating micro/nano-fabrication work to elucidate the molecular mechanism of signaling transduction in cell biology. We first describe different micro/nano-fabrication technologies, including techniques generating three-dimensional scaffolds for tissue engineering. We then introduce the application of these technologies in manipulating the physical or chemical micro/nano-environment to regulate the cellular behavior and response, such as cell life and death, differentiation, proliferation, and cell migration. Recent advancement in integrating the micro/nano-technologies and live cell imaging are also discussed. Finally, potential schemes in cell biology involving micro/nano-fabrication technologies are proposed to provide perspectives on the future research activities.
Vorheriger Artikel Cell sheet integrity and nanomechanical breakdown during programmed cell death
Nächster Artikel Imaging gap junctions with silica-coated upconversion nanoparticles

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Literatur
1.
Abraham LC, Dice JF, Finn PF, Mesires NT, Lee K, Kaplan DL (2007) Extracellular matrix remodeling methods to quantify cell matrix interactions. Biomaterials 28:151–161CrossRefPubMed
2.
Alyassin MA, Moon S, Keles HO, Manzur F, Lin RL, Haeggstrom E, Kuritzkes DR, Demirci U (2009) Rapid automated cell quantification on HIV microfluidic devices. Lab on a Chip 9:3364–3369CrossRefPubMed
3.
Bailly M, Yan L, Whitesides GM, Condeelis JS, Segall JE (1998) Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells. Exp Cell Res 241:285–299CrossRefPubMed
4.
Biggs MJP, Richards RG, Gadegaard N, McMurray RJ, Affrossman S, Wilkinson CDW, Oreffo ROC, Dalby MJ (2009) Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. J Biomed Mater Res A 91A:195–208CrossRef
5.
Boland ED, Matthews JA, Pawlowski KJ, Simpson DG, Wnek GE, Bowlin GL (2004) Electrospinning collagen and elastin: preliminary vascular tissue engineering. Front Biosci 9:1422–1432CrossRefPubMed
6.
Broers AN, Molzen WW, Cuomo JJ, Wittels ND (1976) Electron-beam fabrication of 80 Ǻ metal structures. Appl Phys Lett 29:596–598CrossRef
7.
Buchko CJ, Chen LC, Shen Y, Martin DC (1999) Processing and microstructural characterization of porous biocompatible protein polymer thin films. Polymer 40:7397–7407CrossRef
8.
Carraro A, Hsu WM, Kulig KM, Cheung WS, Miller ML, Weinberg EJ, Swart EF, Kaazempur-Mofrad M, Borenstein JT, Vacanti JP, Neville C (2008) In vitro analysis of a hepatic device with intrinsic microvascular-based channels. Biomed Microdevices 10:795–805CrossRefPubMed
9.
Chan-Park MB, Yang C, Guo X, Chen LQ, Yoon SF, Chun JH (2008) Fabrication of 3-D curved microstructures by constrained gas expansion and photopolymerization. Langmuir 24:5492–5499CrossRefPubMed
10.
Chao SH, Carlson R, Meldrum DR (2007) Rapid fabrication of microchannels using microscale plasma activated templating (mu PLAT) generated water molds. Lab on a Chip 7:641–643CrossRefPubMed
11.
Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Geometric control of cell life and death. Science 276:1425–1428CrossRefPubMed
12.
Cukierman E, Pankov R, Stevens DR, Yamada KM (2001) Taking cell-matrix adhesions to the third dimension. Science 294:1708–1712CrossRefPubMed
13.
Dike LE, Chen CS, Mrksich M, Tien J, Whitesides GM, Ingber DE (1999) Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35:441–448CrossRefPubMed
14.
Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689CrossRefPubMed
15.
16.
Fang X, Reneker DH (1997) DNA fibers by electrospinning. J Macromol Sci Phys B36:169–173
17.
Giang UBT, Lee D, King MR, DeLouise LA (2007) Microfabrication of cavities in polydimethylsiloxane using DRIE silicon molds. Lab on a Chip 7:1660–1662CrossRefPubMed
18.
Gillette BM, Jensen JA, Tang BX, Yang GJ, Bazargan-Lari A, Zhong M, Sia SK (2008) In situ collagen assembly for integrating microfabricated three-dimensional cell-seeded matrices. Nat Mater 7:636–640CrossRefPubMed
19.
Gomez N, Chen SC, Schmidt CE (2007) Polarization of hippocampal neurons with competitive surface stimuli: contact guidance cues are preferred over chemical ligands. J R Soc Interface 4:223–233CrossRefPubMed
20.
Hartman O, Zhang C, Adams EL, Farach-Carson MC, Petrelli NJ, Chase BD, Rabolt JF (2009) Microfabricated electrospun collagen membranes for 3-D cancer models and drug screening applications. Biomacromolecules 10:2019–2032CrossRefPubMed
21.
Hodgson L, Chan EWL, Hahn KM, Yousaf MN (2007) Combining surface chemistry with a FRET-based biosensor to study the dynamics of RhoA GTPase activation in cells on patterned substrates. J Am Chem Soc 129:9264–9265CrossRefPubMed
22.
Hoff JD, Cheng LJ, Meyhofer E, Guo LJ, Hunt AJ (2004) Nanoscale protein patterning by imprint lithography. Nano Lett 4:853–857CrossRef
23.
Hoover DK, Chan EWL, Yousaf MN (2008) Asymmetric peptide nanoarray surfaces for studies of single cell polarization. J Am Chem Soc 130:3280–3281CrossRefPubMed
24.
Hsiong SX, Boontheekul T, Huebsch N, Mooney DJ (2009) Cyclic arginine-glycine-aspartate peptides enhance three-dimensional stem cell osteogenic differentiation. Tissue Eng A 15:263–272CrossRef
25.
Hu SH, Eberhard L, Chen JX, Love JC, Butler JP, Fredberg JJ, Whitesides GM, Wang N (2004) Mechanical anisotropy of adherent cells probed by a three-dimensional magnetic twisting device. Am J Physiol Cell Physiol 287:C1184–C1191CrossRefPubMed
26.
Hwang YS, Chung BG, Ortmann D, Hattori N, Moeller HC, Khademhosseini A (2009) Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci USA 106:16978–16983CrossRefPubMed
27.
Hyun JH, Ma HW, Zhang ZP, Beebe TP, Chilkoti A (2003) Universal route to cell micropatterning using an amphiphilic comb polymer. Adv Mater 15:576–579CrossRef
28.
Jeon NL, Baskaran H, Dertinger SKW, Whitesides GM, Van de Water L, Toner M (2002) Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat Biotechnol 20:826–830
29.
Khetani SR, Bhatia SN (2008) Microscale culture of human liver cells for drug development. Nat Biotechnol 26:120–126CrossRefPubMed
30.
Kim TJ, Seong JH, Ouyang MX, Sun J, Lu SY, Hong JP, Wang N, Wang YX (2009) Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells. J Cell Physiol 218:285–293CrossRefPubMed
31.
Kim TJ, Xu J, Dong R, Lu SY, Nuzzo R, Wang YX (2009) Visualizing the effect of microenvironment on the spatiotemporal RhoA and Src activities in living cells by FRET. Small 5:1453–1459CrossRefPubMed
32.
Kong HJ, Boontheekul T, Mooney DJ (2006) Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype. Proc Natl Acad Sci USA 103:18534–18539CrossRefPubMed
33.
Kwon IK, Kidoaki S, Matsuda T (2005) Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. Biomaterials 26:3929–3939CrossRefPubMed
34.
Lauffenburger DA, Horwitz AF (1996) Cell migration: a physically integrated molecular process. Cell 84:359–369CrossRefPubMed
35.
Lee KB, Park SJ, Mirkin CA (2002) Protein nanoarrays generated by dip-pen nanolithography. Abstr Pap Am Chem Soc 223:506-PHYS
36.
Liu GY, Xu S, Qian YL (2000) Nanofabrication of self-assembled monolayers using scanning probe lithography. Acc Chem Res 33:457–466CrossRefPubMed
37.
Ma HW, Hyun J, Zhang ZP, Beebe TP, Chilkoti A (2005) Fabrication of biofunctionalized quasi-three-dimensional microstructures of a nonfouling comb polymer using soft lithography. Adv Funct Mater 15:529–540CrossRef
38.
Martinez E, Engel E, Planell JA, Samitier J (2009) Effects of artificial micro- and nano-structured surfaces on cell behaviour. Ann Anat Anatomischer Anzeiger 191:126–135CrossRef
39.
Matthews JA, Wnek GE, Simpson DG, Bowlin GL (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3:232–238CrossRefPubMed
40.
McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS (2004) Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6:483–495CrossRefPubMed
41.
Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25:1289–1297CrossRefPubMed
42.
Na S, Collin O, Chowdhury F, Tay B, Ouyang M, Wang Y, Wang N (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc Natl Acad Sci USA 105:6626–6631CrossRefPubMed
43.
Neeley WL, Redenti S, Klassen H, Tao S, Desai T, Young MJ, Langer R (2008) A microfabricated scaffold for retinal progenitor cell grafting. Biomaterials 29:418–426CrossRefPubMed
44.
O’Neill C, Jordan P, Ireland G (1986) Evidence for two distinct mechanisms of anchorage stimulation in freshly explanted and 3T3 Swiss mouse fibroblasts. Cell 44:489–496CrossRefPubMed
45.
Ohgo K, Zhao CH, Kobayashi M, Asakura T (2003) Preparation of non-woven nanofibers of Bombyx mori silk, Samia cynthia ricini silk and recombinant hybrid silk with electrospinning method. Polymer 44:841–846CrossRef
46.
Ouyang MX, Sun J, Chien S, Wang YX (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors. Proc Natl Acad Sci USA 105:14353–14358CrossRefPubMed
47.
Park J, Toner M, Yarmush ML, Tilles AW (2006) Microchannel bioreactors for bioartificial liver support. Microfluid Nanofluid 2:525–535CrossRef
48.
Park JY, Hwang CM, Lee SH (2009) Ice-lithographic fabrication of concave microwells and a microfluidic network. Biomed Microdevices 11:129–133CrossRefPubMed
49.
Petrie RJ, Doyle AD, Yamada KM (2009) Random versus directionally persistent cell migration. Nat Rev Mol Cell Biol 10:538–549CrossRefPubMed
50.
Pham QP, Sharma U, Mikos AG (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12:1197–1211CrossRefPubMed
51.
Piner RD, Zhu J, Xu F, Hong SH, Mirkin CA (1999) “Dip-pen” nanolithography. Science 283:661–663CrossRefPubMed
52.
Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR (2003) Cell migration: integrating signals from front to back. Science 302:1704–1709CrossRefPubMed
53.
Rogers JA, Nuzzo RG (2005) Recent progress in soft lithography. Mater Today 8:50–56CrossRef
54.
Ruiz SA, Chen CS (2008) Emergence of patterned stem cell differentiation within multicellular structures. Stem Cells 26:2921–2927CrossRefPubMed
55.
Saadi W, Rhee SW, Lin F, Vahidi B, Chung BG, Jeon NL (2007) Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber. Biomed Microdevices 9:627–635CrossRefPubMed
56.
Seong J, Lu S, Ouyang M, Huang H, Zhang J, Frame MC, Wang Y (2009) Visualization of Src activity at different compartments of the plasma membrane by FRET imaging. Chem Biol 16:48–57CrossRefPubMed
57.
Shiu YT, Li S, Yuan S, Wang Y, Nguyen P, Chien S (2003) Shear stress-induced c-fos activation is mediated by Rho in a calcium-dependent manner. Biochem Biophys Res Commun 303:548–555CrossRefPubMed
58.
Subbiah T, Bhat GS, Tock RW, Pararneswaran S, Ramkumar SS (2005) Electrospinning of nanofibers. J Appl Polym Sci 96:557–569CrossRef
59.
Takayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM (2001) Laminar flows: subcellular positioning of small molecules. Nature 411:1016CrossRefPubMed
60.
Wang YX, Wang N (2009) FRET and mechanobiology. Integr Biol 1:565–573CrossRef
61.
Webb A, Clark P, Skepper J, Compston A, Wood A (1995) Guidance of oligodendrocytes and their progenitors by substratum topography. J Cell Sci 108:2747–2760PubMed
62.
Wood A (1988) Contact guidance on microfabricated substrata: the response of teleost fin mesenchyme cells to repeating topographical patterns. J Cell Sci 90:667–681PubMed
63.
Yasin S, Hasko DG, Ahmed H (2001) Fabrication of <5 nm width lines in poly(methylmethacrylate) resist using a water:isopropyl alcohol developer and ultrasonically-assisted development. Appl Phys Lett 78:2760–2762CrossRef
64.
Yi Y, Kang JH, Park JK (2005) Moldless electroplating for cylindrical microchannel fabrication. Electrochem Commun 7:913–917
65.
Yu J, Xiao J, Ren XJ, Lao KQ, Xie XS (2006) Probing gene expression in live cells, one protein molecule at a time. Science 311:1600–1603CrossRefPubMed
66.
Zhou D, Bruckbauer A, Ying LM, Abell C, Klenerman D (2003) Building three-dimensional surface biological assemblies on the nanometer scale. Nano Lett 3:1517–1520CrossRef
Metadaten
Titel
Micro/nano-fabrication technologies for cell biology
verfasst von
Tongcheng Qian
Yingxiao Wang
Publikationsdatum
01.10.2010
Verlag
Springer-Verlag
Erschienen in
Medical & Biological Engineering & Computing / Ausgabe 10/2010
Print ISSN: 0140-0118
Elektronische ISSN: 1741-0444
DOI
https://doi.org/10.1007/s11517-010-0632-z

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