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Microsystem Technologies

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01.10.2010 | Technical Paper

Manufacturing process and thermal characterization of a fast temperature switching microdevice for real-time biological experiments

verfasst von: Frederic Gillot, Hideyuki F. Arata, Fabrice O. Morin, Hiroyuki Fujita

Erschienen in: Microsystem Technologies | Ausgabe 10/2010

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Abstract

Rapid switching and measurement of the temperature in a small volume of liquid are of paramount importance for the real-time observation of biomolecular events such as protein folding–unfolding or enzymatic reactions. We present a microthermodevice for high-speed biological measurements that combines a heater for fast temperature shifting (millisecond range) and a Thin Film Thermocouple (TFTC) for accurate temperature measurement during microscopic observation. Device manufacturing as well as its thermal characterization are shown. The device is successfully employed to measure the fluorescent quenching of double-stranded DNA coupled with fluorescent intercalator on millisecond time scale, thus demonstrating its capacity for innovative biological experiments.

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Titel: Manufacturing process and thermal characterization of a fast temperature switching microdevice for real-time biological experiments
verfasst von: Frederic Gillot
Hideyuki F. Arata
Fabrice O. Morin
Hiroyuki Fujita
Publikationsdatum: 01.10.2010
Verlag: Springer-Verlag
Erschienen in: Microsystem Technologies / Ausgabe 10/2010
Print ISSN: 0946-7076
Elektronische ISSN: 1432-1858
DOI: https://doi.org/10.1007/s00542-010-1095-8

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