Microwave assisted quaternized cyclodextrin grafted chitosan (QCD-g-CH) nanoparticles entrapping ciprofloxacin

In the present study quaternized cyclodextrin grafted chitosan (QCD-g-CH) nanoparticles entrapping ciprofloxacin were prepared for the development of sustained release system. The QCD-g-CH is prepared by microwave assisted synthesis technique using 3-chloro-2-hydroxypropyl trimethyl ammonium chloride as cationic reagent. The QCD-g-CH entrapping ciprofloxacin nanoparticles are prepared by ionic gelation method using NaTPP as a cross linker. The prepared QCD-g-CH nanoparticles were characterized by FTIR, DSC, X-RD and FESEM-EDS. The average p-size of the drug loaded QCD-g-CH nanoparticles was found in the range 176 to 642 nm whereas the % encapsulation efficiency of different batches was found to be in the range of 80 to 92%. The % degree of tosylation, substitution and quaternization were calculated by ¹H NMR and it was found to be1.27, 5.07 and 66.66%. The antimicrobial activity was studied using bacterial and fungal strains. The in vitro release study of QCD-g-CH-CPFX NPs in PBS buffer (pH 7.4) showed sustained release behavior during 24 h study.