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2024 | OriginalPaper | Buchkapitel

Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material

verfasst von : Andrey Matrosov, Arkady Soloviev, Irina Serebryanaya, Olga Pustovalova, Daria Nizhnik

Erschienen in: Physics and Mechanics of New Materials and Their Applications

Verlag: Springer Nature Switzerland

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Abstract

This study focuses on the advancement of new techniques for cryopreservation of reproductive cells at low temperatures, including sturgeon fish. The approach is based on smart freezing process control. To ensure cryoprotectant and reproductive cells are not damaged prior to vitrification, a piezoactuator is used to stir the mixture. This technique facilitates the intensive mixing of cryoprotectant, promoting the diffusion of the substance through the cell membrane. During direct vitrification, researchers observe a phase transition phenomenon in the presence of crystal formation. This study constructs a mathematical model to investigate this process while comparing numerical experiments to in situ experiments. The results obtained have implications for selecting optimal parameters required for the low-temperature preservation of fish reproductive cells.

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Literatur
10.
Zurück zum Zitat Huang, X., et al.: Effects of cryopreservation on acrosin activity and DNA damage of Russian sturgeon (Acipenser gueldenstaedtii) semen. CryoLetters 42(3), 129–136 (2021)PubMed Huang, X., et al.: Effects of cryopreservation on acrosin activity and DNA damage of Russian sturgeon (Acipenser gueldenstaedtii) semen. CryoLetters 42(3), 129–136 (2021)PubMed
12.
Zurück zum Zitat Andreev, A.A., Sadikova, D.G., Ponomareva, E.N., Krasilnikova, A.A., Belaya, M.: Method of reduction of low temperature jump of cryoprotectant solutions. Patent RU 2540598 C2 (2015). (in Russian) Andreev, A.A., Sadikova, D.G., Ponomareva, E.N., Krasilnikova, A.A., Belaya, M.: Method of reduction of low temperature jump of cryoprotectant solutions. Patent RU 2540598 C2 (2015). (in Russian)
17.
Zurück zum Zitat Matrosov, A.A., Nizhnik, D.A., Ponomareva, E.N., Soloviev, A.N.: Modeling the impact of piezoactuator on fish reproductive cells in the cryoprotective environment. In: State and Prospects of Agroindustrial Complex “Interagro-2022”, Rostov-on-Don, pp. 266–269 (2022). (in Russian). https://doi.org/10.23947/interagro.2022.266-269 Matrosov, A.A., Nizhnik, D.A., Ponomareva, E.N., Soloviev, A.N.: Modeling the impact of piezoactuator on fish reproductive cells in the cryoprotective environment. In: State and Prospects of Agroindustrial Complex “Interagro-2022”, Rostov-on-Don, pp. 266–269 (2022). (in Russian). https://​doi.​org/​10.​23947/​interagro.​2022.​266-269
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Zurück zum Zitat Matrosov, A., Nizhnik, D., Pakhomov, V., Soloviev, A.: Diffusion of cryoprotectant through the membrane of reproductive cells during equilibration. In: Parinov, I.A., Chang, S.-H., Soloviev, A.N. (eds.) Physics and Mechanics of New Materials and Their Applications (PHENMA), vol. 20, pp. 508–514. Springer, Cham (2023). https://doi.org/10.1007/978-3-031-21572-8_44CrossRef Matrosov, A., Nizhnik, D., Pakhomov, V., Soloviev, A.: Diffusion of cryoprotectant through the membrane of reproductive cells during equilibration. In: Parinov, I.A., Chang, S.-H., Soloviev, A.N. (eds.) Physics and Mechanics of New Materials and Their Applications (PHENMA), vol. 20, pp. 508–514. Springer, Cham (2023). https://​doi.​org/​10.​1007/​978-3-031-21572-8_​44CrossRef
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Zurück zum Zitat Blagin, A.V., Blagina, L.V., Knyazev, S.Yu., Shcherbakova, E.E., Nefyodova, N.A.: Crystallization processes in gradient fields. In: Mathematical Models and Experiment, Rostov-on-Don (2021). (in Russian) Blagin, A.V., Blagina, L.V., Knyazev, S.Yu., Shcherbakova, E.E., Nefyodova, N.A.: Crystallization processes in gradient fields. In: Mathematical Models and Experiment, Rostov-on-Don (2021). (in Russian)
Metadaten
Titel
Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material
verfasst von
Andrey Matrosov
Arkady Soloviev
Irina Serebryanaya
Olga Pustovalova
Daria Nizhnik
Copyright-Jahr
2024
DOI
https://doi.org/10.1007/978-3-031-52239-0_12