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Biomass Conversion and Biorefinery

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22.04.2022 | Original Article

Optimization of preparation of Candida utilis polypeptide by ultrasonic pretreatment and double enzyme method

verfasst von: Lingling Wang, Yan He, Lihua Chen, Xia Ma

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 3/2024

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Abstract

Candida utilis is a high-yielding strain of single-cell protein and a good source of bioactive peptides. At present, the research on Candida utilis mainly focuses on using it as animal feed, which greatly limits the application of Candida utilis. The aim of this work was to study the enzymatic hydrolysis of Candida utilis by ultrasonic pretreatment double-enzymatic method to maximize the enzymatic hydrolysis yield. For this purpose, a response surface method (RSM) was used, with enzyme dosage, enzyme ratio, temperature, pH, and time as process variables. The analysis of variance (ANOVA) results suggested that enzyme ratio, temperature, and pH were the most significant factors affecting the polypeptide yield and degree of hydrolysis. Response surface modelling showed that optimal polypeptide yield and degree of hydrolysis were achieved at the ratio of enzyme (alkaline protease: trypsin) 1: 1.85, the enzymolysis temperature 51 °C, and the pH 10. The optimal yields of polypeptide and hydrolysis degree were 53.34% and 47.78%, respectively. After ultrafiltration of Candida utilis hydrolysate, the polysaccharide removal rate reached 57.09 ± 2.69%, and the free radical scavenging activity was significantly improved. Infrared spectrum results showed that the Candida utilis polypeptides prepared in this study have obvious amide I, II, and III band structure. Therefore, ultrasonic-assisted double-enzyme method can be effectively used to prepare polypeptides from Candida utilis and has the potential to be applied in the industrial extraction of Candida utilis polypeptides to increase the added value of yeast.

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Chandana S, Bababode AK, Poorva S (2022) Isolation and functionalities of bioactive peptides from fruits and vegetables: a review[J]. Food Chem 366:130494. https://doi.org/10.1016/j.foodchem.2021.130494CrossRef

Huang Y, Ruan G, Qin Z et al (2017) Antioxidant activity measurement and potential antioxidant peptides exploration from hydrolysates of novel continuous microwave-assisted enzymolysis of the Scomberomorus niphonius protein[J]. Food Chem 223:89–95. https://doi.org/10.1016/j.foodchem.2016.12.026CrossRef

Ketnawa S, Martínez-Alvarez O, Benjakul S et al (2016) Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion[J]. Food Chem 192:34–42. https://doi.org/10.1016/j.foodchem.2015.06.087CrossRef

Marson GV, Lacour S, Hubinger MD et al (2022) Serial fractionation of spent brewer’s yeast protein hydrolysate by ultrafiltration: a peptide-rich product with low RNA content[J]. J Food Eng 312:110737. https://doi.org/10.1016/j.jfoodeng.2021.110737CrossRef

Abdullah FI, Chua LS, Rahmat Z (2017) Comparison of protein extraction methods for the leaves of Ficus deltoidea[J]. J Fund Applied Sci 9(2):908–924. https://doi.org/10.4314/jfas.v9i2.19CrossRef

Pihlanto A, Mattila P, Mäkinen S et al (2017) Bioactivities of alternative protein sources and their potential health benefits[J]. FOOD FUNCT 8(10):3443–3458. https://doi.org/10.1039/C7FO00302ACrossRef

Virtanen H E K, Voutilainen S, Koskinen T T et al (2019) Dietary proteins and protein sources and risk of death: the Kuopio Ischaemic Heart Disease Risk Factor Study[J]. Am J Clin Nutr 109(5): 1462–1471. https://doi.org/10.1093/ajcn/nqz025

Ferreira IMPLVO, Pinho O, Vieira E et al (2009) Brewer’s Saccharomyces yeast biomass: characteristics and potential applications[J]. Trends Food Sci Tech 21(2):77–84. https://doi.org/10.1016/j.tifs.2009.10.008CrossRef

Dimitrellou D, Kandylis P, Kourkoutas Y et al (2008) Evaluation of thermally-dried Kluyveromyces marxianus as baker’s yeast[J]. Food Chem 115(2):691–696. https://doi.org/10.1016/j.foodchem.2008.12.050CrossRef

10.

Hoz Ldl, Ponezi AN, Milani RF et al (2014) Iron-binding properties of sugar cane yeast peptides[J]. Food Chem 142:166–169. https://doi.org/10.1016/j.foodchem.2013.06.133CrossRef

11.

Paš M, Piškur B, Šuštarič M et al (2007) Iron enriched yeast biomass–a promising mineral feed supplement[J]. Bioresource Technol 98(8):1622–1628. https://doi.org/10.1016/j.biortech.2006.06.002CrossRef

12.

Yamada EA, Sgarbieri VC (2005) Yeast (Saccharomyces cerevisiae) protein concentrate: preparation, chemical composition, and nutritional and functional properties[J]. J Agric Food Chem 53(10):3931–3936. https://doi.org/10.1021/jf0400821CrossRef

13.

Hengke G, Siyu G, Hongmei L (2020) Antioxidant activity and inhibition of ultraviolet radiation-induced skin damage of Selenium-rich peptide fraction from selenium-rich yeast protein hydrolysate[J]. Bioorg Chem 105:104431. https://doi.org/10.1016/j.bioorg.2020.104431CrossRef

14.

Liu D, Zeng XA, Sun DW et al (2013) Disruption and protein release by ultrasonication of yeast cells[J]. Innov Food Sci and Emer 18:132–137. https://doi.org/10.1016/j.ifset.2013.02.006CrossRef

15.

A. Y.E C. S.V (2005) Yeast (Saccharomyces cerevisiae) protein concentrate: preparation, chemical composition. and nutritional and functional properties. J Agr Food Chem 53 (10): 3931–3936 https://doi.org/10.1021/jf0400821

16.

Caballero-Córdoba GM, Sgarbieri VC (2000) Nutritional and toxicological evaluation of yeast ( Saccharomyces cerevisiae ) biomass and a yeast protein concentrate[J]. J Sci Food Agr 80(3):341–351. https://doi.org/10.1002/1097-0010(200002)80:3%3c341::AID-JSFA533%3e3.0.CO;2-MCrossRef

17.

Lamoolphak W, Goto M, Sasaki M et al (2006) A Shotipruk Hydrothermal decomposition of yeast cells for production of proteins and amino acids[J]. J Hazad mater 137(3):1643–1648. https://doi.org/10.1016/j.jhazmat.2006.05.029CrossRef

18.

Mirzaei M, Aminlari M, Hosseini E (2016) Antioxidant, ACE-inhibitory and antimicrobial activities of Kluyveromyces marxianus protein hydrolysates and their peptide fractions[J]. Funct Foods Health D 6(7):425–439. https://doi.org/10.31989/ffhd.v6i7.250CrossRef

19.

Marson G V, da Costa Machado M T, de Castro R J S et al (2019) Sequential hydrolysis of spent brewer’s yeast improved its physico-chemical characteristics and antioxidant properties: a strategy to transform waste into added-value biomolecules[J]. Process Biochem 84:91–102. https://doi.org/10.1016/j.procbio.2019.06.018CrossRef

20.

Middelberg APJ (1995) Process-scale disruption of microorganisms[J]. BIOTECHNOL ADV 13(3):491–551. https://doi.org/10.1016/0734-9750(95)02007-PCrossRef

21.

Tacias-Pascacio VG, Castañeda-Valbuena D, Morellon-Sterling R et al (2021) Bioactive peptides from fisheries residues: a review of use of papain in proteolysis reactions[J]. Int J of Biol Macromol 184:415–428. https://doi.org/10.1016/j.ijbiomac.2021.06.076CrossRef

22.

Tacias-Pascacio VG, Morellon-Sterling R, Siar EH et al (2020) Use of Alcalase in the production of bioactive peptides: a review[J]. Int J of Biol Macromol 165:2143–2196. https://doi.org/10.1016/j.ijbiomac.2020.10.060CrossRef

23.

Tavano OL, Berenguer-Murcia A, Secundo F et al (2018) Biotechnological applications of proteases in food technology[J]. Compr Rev Food Sci F 17(2):412–436. https://doi.org/10.1111/1541-4337.12326CrossRef

24.

Marson GV, de Castro RJS, da Costa Machado MT et al (2020) Proteolytic enzymes positively modulated the physicochemical and antioxidant properties of spent yeast protein hydrolysates[J]. Process Biochem 91:34–45. https://doi.org/10.1016/j.procbio.2019.11.030CrossRef

25.

Yang L, Guo Z, Wei J et al (2021) Extraction of low molecular weight peptides from bovine bone using ultrasound-assisted double enzyme hydrolysis: impact on the antioxidant activities of theextracted peptides[J]. LWT 146:111470. https://doi.org/10.1016/j.lwt.2021.111470CrossRef

26.

Wen C, Zhang J, Zhou J et al (2020) Antioxidant activity of arrowhead protein hydrolysates produced by a novel multi-frequency S-type ultrasound-assisted enzymolysis. Nat Prod Res 34(20):3000–3003. https://doi.org/10.1080/14786419.2019.1601192CrossRef

27.

Ulug S K, Jahandideh F, Wu J (2020) Novel technologies for the production of bioactive peptides. Trends Food Sci Tech 108(2020):27–39. https://doi.org/10.1016/j.tifs.2020.12.002

28.

Wen C, Zhang J, Zhou J et alet al (2020) Antioxidant activity of arrowhead protein hydrolysates produced by a novel multi-frequency S-type ultrasound-assisted enzymolysis. Nat Prod Res 34(20):3000–3003. https://doi.org/10.1080/14786419.2019.1601192

29.

Guo H, Guo S, Liu H (2020) Antioxidant activity and inhibition of ultraviolet radiation-induced skin damage of Selenium-rich peptide fraction from selenium-rich yeast protein hydrolysate. Bioor Chem 105:104431. https://doi.org/10.1016/j.bioorg.2020.104431

30.

Wu Y (2014) Preparation and Activity of Tyrosinase-Inhibitory Peptide from Yeast[D], Jiangnan University. JiangSu (in Chinese)CrossRef

31.

Zhang L , Shi C, Xiao K et al (2019) Improvement and Application of Biuret Methodfor Determination of Collagen Peptide from Tilapia. Food Sci 40(20):234–240. https://doi.org/10.7506/spkx1002-6630-20181118-206

32.

Jadhav S B, Gaonkar T, Rathi A (2021) In vitro gastrointestinal digestion of proteins in the presence of enzyme supplements: Details of antioxidant and antidiabetic properties. LWT 147:111650. https://doi.org/10.1016/j.lwt.2021.111650

33.

Luo Y, Wang Q, Chen P et al (2017) Study on Determination Method for Hydrolyzed De gree of Protein Hydrolysate. Leather and Chem 34(02):26–31 2 (in Chinese)

34.

Chen C (2011) Isolation, Purification and Bioactivities of Polysaccharide from Pichia pastoris[D]. Dalian University of Technology. LiaoNing (in Chinese)

35.

Ren K (2016) Purification of pneumococcal capsular polysaccharides Type 14 by REN Keming[D]. Lanzhou University of Technology. Gan Su (in Chinese)CrossRef

36.

Wang G (2013) Preparations of peptide and polysaccharide and activitiesresearch of Apostichopus japonicus Spawn peptide[D]. Shanghai Ocean University. ShangHai (in Chinese)CrossRef

37.

Dubois M, Gilles K A, Hamilton J K, et al (1956) Colorimetric method for determination of sugars and related substances. Analy chem 28(3):350–356.

38.

Fu R, Zhang Y, Guo Y et al (2014) Antioxidant and tyrosinase inhibition activities of the ethanol-insoluble fraction of water extract of Sapium sebiferum (L.) Roxb. leaves[J]. S AFR J BOT 93:98–104. https://doi.org/10.1016/j.sajb.2014.04.003

39.

Ahmadi F, Kadivar M, Shahedi M (2007) Antioxidant activity of Kelussia odoratissima Mozaff. in model and food systems. Food Chem 105(1):57–64. https://doi.org/10.1016/j.foodchem.2007.03.056

40.

Wang LL, Xiong Y L (2005) Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. J Agr Food Chem 53(23):9186–9192

41.

Guo Z, Xu L, Song J et al (2016) Functional Exploration and Application of Candida Utilis. Feed Rev (3):33–35 (in Chinese)

42.

Lin Z, Jiao G, Zhang J et al (2020) Optimization of protein extraction from bamboo shoots and processing wastes using deep eutectic solvents in a biorefinery approach. Biomass Conver Bior 1–12. https://doi.org/10.1007/s13399-020-00614-3

43.

Wu T, Yu X, Hu A et al (2015) Ultrasonic disruption of yeast cells: Underlying mechanism and effects of processing parameters. Innov Food Sci Emerg 28:59–65. https://doi.org/10.1016/j.ifset.2015.01.005

44.

Zhao J, Fleet G (2003) Degradation of DNA during the autolysis of Saccharomyces cerevisiae. J Ind. Microbiol Biotechnol 30:175–182. https://doi.org/10.1007/s10295-003-0028-2

45.

Liu D, Zeng X A, Sun D W et al (2013) Disruption and proteins release by ultrasonication of yeast cells. Innov Food Sci Emerg 18:132–137. https://doi.org/10.1016/j.ifset.2013.02.006

46.

Görgüç A, Özer P, Yılmaz FM (2020) Simultaneous effect of vacuum and ultrasound assisted enzymatic extraction on the recovery of plant protein and bioactive compounds from sesame bran. J Food Compos Anal 87:103424. https://doi.org/10.1016/j.jfca.2020.103424

47.

Wen C, Zhang J, Zhou J et al (2020) Slit divergent ultrasound pretreatment assisted watermelon seed protein enzymolysis and the antioxidant activity of its hydrolysates in vitro and in vivo. Food Chem 328:127135. https://doi.org/10.1016/j.foodchem.2020.127135

48.

Xu X, Qiao Y, Shi B et al (2021) Alcalase and bromelain hydrolysis affected physicochemical and functional properties and biological activities of legume proteins. Food Struct-Nete 27:100178. https://doi.org/10.1016/j.foostr.2021.100178

49.

Kula E, Kocadag Kocazorbaz E, Moulahoum H et al (2020) Extraction and characterization of novel multifunctional peptides from Trachinus Draco (greater weever) myofibrillar proteins with ACE/DPP4 inhibitory, antioxidant, and metal chelating activities. J Food biochem 44(5):e13179. https://doi.org/10.1111/jfbc.13179

50.

Poy H, Lladosa E, Gabaldón C et al (2021) Optimization of rice straw pretreatment with 1-ethyl-3-methylimidazolium acetate by the response surface method. Biomass Conv Bioref 1–16. https://doi.org/10.1007/s13399-021-02111-7

51.

Ma T, Fu Q, Mei Q et al (2021) Extraction optimization and screening of angiotensin-converting enzyme inhibitory peptides from Channa striatus through bioaffinity ultrafiltration coupled with LC-Orbitrap-MS/MS and molecular docking. Food Chem 354:129589. https://doi.org/10.1016/j.foodchem.2021.129589

52.

Wu W, He L, Liang Y et al (2019) Preparation process optimization of pig bone collagen peptide-calcium chelate using response surface methodology and its structural characterization and stability analysis. Food chem 284:80–89. https://doi.org/10.1016/j.foodchem.2019.01.103

53.

Chakraborty S, Shrivastava C (2019) Comparison between multiresponse‐robust process design and numerical optimization: A case study on baking of fermented chickpea flour‐based wheat bread. J Food Process Eng 42(3):e13008. https://doi.org/10.1111/jfpe.13008

54.

Li C, Liu J, Gao Y et al (2020) Influencing Factors of Determination of Nucleic Acid Content by Ultraviolet Spectrophotometry. Chem Reag 42(01):53–57. https://doi.org/10.13822/j.cnki.hxsj.2020007049

55.

Li J, Jin L, Weng Z et al (2008) Study of Ultraviolet absorption Spectra of Guanine, Adenine, Thymine and Cytosine. J Changchun Univ Sci Tech (Natural Science Edition) (02):74–76 (in Chinese)

56.

Marson GV, Pereira DTV, da Costa Machado MT et al (2021) Ultrafiltration performance of spent brewer's yeast protein hydrolysate: Impact of pH and membrane material on fouling. J Food Eng 302:110569. https://doi.org/10.1016/j.jfoodeng.2021.110569

57.

Chen G, Chen Y, Hou Y et al (2020) Preparation, characterization and the in vitro bile salts binding capacity of celery seed protein hydrolysates via the fermentation using B. subtilis. LWT 117:108571. https://doi.org/10.1016/j.lwt.2019.108571

58.

Liang D, Chen F, Zhang L et al (2021) The Isolation and Purification of Muropeptides from the Bacillus subtilis and Its Effect on Spore Germination. J Chin Ins of Food Sci and Tech 21(03):369–374. https://doi.org/10.16429/j.1009-7848.2021.03.042

59.

Li C, Lu B, Wang C (2018) Preparation and structure analysis of rice protein antioxidant peptides. Chin Food Add (09):84–91 (in Chinese)

60.

Gao J, Li T, Chen D et al (2021) Identification and molecular docking of antioxidant peptides from hemp seed protein hydrolysates. LWT 147:111453. https://doi.org/10.1016/j.lwt.2021.111453

61.

Li M, Fan W, Xu Y (2021) Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste. LWT 151:112221. https://doi.org/10.1016/j.lwt.2021.112221

62.

Wattanasiritham L, Theerakulkait C, Wickramasekara S et al (2016) Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein. Food Chem 192:156–162. https://doi.org/10.1016/j.foodchem.2015.06.057

63.

Mirzaei M, Mirdamadi S, Ehsani MR et al (2018) Production of antioxidant and ACE-inhibitory peptides from Kluyveromyces marxianus protein hydrolysates: Purification and molecular docking. J Food Drug Anal 26(2):696–705. https://doi.org/10.1016/j.jfda.2017.07.008

64.

Mirzaei M, Mirdamadi S, Ehsani MR et al (2015) Purification and identification of antioxidant and ACE-inhibitory peptide from Saccharomyces cerevisiae protein hydrolysate. J Funct Foods 19:259–268. https://doi.org/10.1016/j.jff.2015.09.031

65.

Bi Q (2019) Study on the Preparation and Antioxidant Activity of Polypeptidesfrom Undaria pinnatifida. Chin Condi 44(05):104–110.

66.

Kaur R, Arora S, Singh B (2008) Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A. Juss. Bioresource Technol 99(16):7692–7698. https://doi.org/10.1016/j.biortech.2008.01.070

67.

Lin M, Yu JZ (2020) Assessment of interactions between transition metals and atmospheric organics: Ascorbic acid depletion and hydroxyl radical formation in organic-metal mixtures. Environ Sci Technol 54(3):1431–1442. https://doi.org/10.1021/acs.est.9b07478

68.

Malomo SA, Onuh JO, Girgih AT et al (2015) Structural and antihypertensive properties of enzymatic hemp seed protein hydrolysates. Nutrients 7(9):7616–7632. https://doi.org/10.3390/nu7095358

69.

Zou Y, Lu Y, Wei D (2004) Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. J Agr Food Chem 52(16):5032–5039. https://doi.org/10.1021/jf049571r

70.

Zhuang Y, Zhang Y (2014) Preparation and Purification of Antioxidant Peptide from Tilapia Skin. J Kunming Univer Sci Tech (Natural Sciences) 39(01):87–93 (in Chinese)

71.

Ko JY, Lee JH, Samarakoon K et al (2013) Purification and determination of two novel antioxidant peptides from flounder fish (Paralichthys olivaceus) using digestive proteases. Food Chem Toxicol 52:113–120. https://doi.org/10.1016/j.fct.2012.10.058

72.

Liu Y, Huang G (2018) The derivatization and antioxidant activities of yeast mannan. Int J Bio Macromol 107:755–761. https://doi.org/10.1016/j.ijbiomac.2017.09.055

73.

Liu Y, Wu Q, Wu X et al (2021) Structure, preparation, modification, and bioactivities of β-glucan and mannan from yeast cell wall: A review. Int J Biol Macromol 173:445–456. https://doi.org/10.1016/j.ijbiomac.2021.01.125

74.

Zou TB, He TP, Li HB et al (2016) The structure-activity relationship of the antioxidant peptides from natural proteins. Molecules 21(1):72. https://doi.org/10.3390/molecules21010072

75.

Xia Y, Bamdad F, Gänzle M et al (2012) Fractionation and characterization of antioxidant peptides derived from barley glutelin by enzymatic hydrolysis. Food Chem 134(3):1509–1518. https://doi.org/10.1016/j.foodchem.2012.03.063

76.

Zhu BW, Zhou DY, Li T et al (2010) Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino). Food Chem 121:712–718. https://doi.org/10.1016/j.foodchem.2010.01.010

Titel: Optimization of preparation of Candida utilis polypeptide by ultrasonic pretreatment and double enzyme method
verfasst von: Lingling Wang
Yan He
Lihua Chen
Xia Ma
Publikationsdatum: 22.04.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Biomass Conversion and Biorefinery / Ausgabe 3/2024
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-022-02652-5

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