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Polymer Bulletin

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06.10.2023 | ORIGINAL PAPER

Preparation and characterization of stable biopolymer-based nanogel for studying its application in immobilization of Candida rugosa lipase

verfasst von: Soumya Sharma, Rachana Sahney, Praveen Dahiya

Erschienen in: Polymer Bulletin | Ausgabe 5/2024

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Abstract

Iron alginate (Fe–Alg) nanogel with a diameter of 96–100 nm was synthesized with the water-in-oil microemulsion method. Candida rugosa lipase (CrL) is hereby chosen as the model enzyme and was immobilized for the first time on biocompatible support of iron alginate nanogel. The structural and morphological properties of immobilized CrL were confirmed by dynamic light scattering, Fourier transform infrared spectroscopy, and scanning electron microscopy. A comparative study between Candida rugosa lipase and immobilized Candida rugosa lipase is provided concerning optimum pH and temperature, thermal and storage stabilities. Various kinetic parameters were scrutinized for increasing the stability, specificity, and cost-effectiveness of Candida rugosa lipase. The temperature optima observed was 35 °C for Candida rugosa lipase which enhanced to 50 °C in the case of immobilized Candida rugosa lipase. The apparent Michalis–Menten constant, K_m (1.5 mM), and maximum velocity, V_max (178.57 µmol/min), values of the immobilized Candida rugosa lipase were significantly changed compared to the free Candida rugosa lipase. Immobilized Candida rugosa lipase showed enhanced solvent and storage stability and retained most of its activity after reusing it for 8 cycles. Molecular docking was performed to study the interaction between amino acids present in Candida rugosa lipase and residues of alginate. The novel immobilized Candida rugosa lipase can be a promising biocatalyst with potential applications in food industries.

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Vorheriger Artikel In vitro release study of electrospun poly(ε-caprolactone)/gelatin nanofiber mats loaded with 5-fluorouracil

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Titel: Preparation and characterization of stable biopolymer-based nanogel for studying its application in immobilization of Candida rugosa lipase
verfasst von: Soumya Sharma
Rachana Sahney
Praveen Dahiya
Publikationsdatum: 06.10.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Polymer Bulletin / Ausgabe 5/2024
Print ISSN: 0170-0839
Elektronische ISSN: 1436-2449
DOI: https://doi.org/10.1007/s00289-023-05009-8

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