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Erschienen in:

2014 | OriginalPaper | Buchkapitel

131. Prevalence Investigation of Tetracycline Resistant Bacteria in Raw Milk

verfasst von : Xiaomei Zhang, Hongjiang Yang

Erschienen in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Verlag: Springer Berlin Heidelberg

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Abstract

To evaluate the prevalence of tetracycline resistant bacteria in raw milk, totally 198 raw milk samples were collected from Hohhot, Inner Mongolia. Preliminary screening results showed that 187 isolated strains were resistant to tetracycline. Fifteen strains were randomly selected for comparative 16S rRNA analysis, and they belonged to different genera including Serratia (5), Pseudomonas (4), Bacillus (1), Providencia (1), Enterobacter (1), Staphylococcus (1), Acinetobacter (1), and one unclassified Bacillales strain, respectively. Their tetracycline resistance phenotype was further validated with K–B disk diffusion method, and 11 strains were resistant to tetracycline and 4 isolates intermediate. Tetracycline resistant determinants were investigated by PCR method. Gene tetAC, tetB, and tetK were found in 11, 8, and 1 isolates, respectively. Furthermore, their hemolytic phenotypes were also characterized. The results showed that tetracycline resistant bacteria with a relatively high diversity commonly existed in raw milk with a high prevalence.

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Titel: Prevalence Investigation of Tetracycline Resistant Bacteria in Raw Milk
verfasst von: Xiaomei Zhang
Hongjiang Yang
Verlag: Springer Berlin Heidelberg
Buch: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)
Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright-Jahr: 2014
DOI: https://doi.org/10.1007/978-3-642-37922-2_131

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