Skip to main content
Erschienen in: Cellulose 5/2015

01.10.2015 | Original Paper

Production of hydroxyapatite–bacterial cellulose nanocomposites from agroindustrial wastes

verfasst von: Eden B. Duarte, Bruna S. das Chagas, Fábia K. Andrade, Ana I. S. Brígida, Maria F. Borges, Celli R. Muniz, Men de Sá M. Souza Filho, João P. S. Morais, Judith P. A. Feitosa, Morsyleide F. Rosa

Erschienen in: Cellulose | Ausgabe 5/2015

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

In the present work, bionanocomposites based on bacterial cellulose (BC) obtained from alternative sources (cashew juice and sisal liquid waste) and hydroxyapatite (HA) were developed. BC–HA composites were prepared through alternate immersion in CaCl2 and Na2HPO4 solutions. Cellulose was successfully produced from the alternative sources of media without the need for additional supplementation and HA crystals that homogeneously precipitated onto the BC surface. The Ca/P ratio ranged from 1.53 to 1.58, indicating the presence of calcium-deficient HA in the composites; this is a phase similar to biological apatite. After immersion into synthetic body fluid, the HA layer formed on the surface of pure BC and the composites, attesting the material’s bioactivity. Moreover, apatite deposition on the composites was up to three times higher than observed on pure cellulose with no significant desorption of apatite from the composites. These results support that the BC derived from agroindustrial wastes have potential to produce nanocomposites of cellulose/HA for use in bone tissue regeneration.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Literatur
Zurück zum Zitat Alge DL, Goebel WS, Chu TMG (2012) In vitro degradation and cytocompatibility of dicalcium phosphate dihydrate cements prepared using the monocalcium phosphate monohydrate/hydroxyapatite system reveals rapid conversion to HA as a key mechanism. J Biomed Mater Res B Appl Biomater 100B:595–602CrossRef Alge DL, Goebel WS, Chu TMG (2012) In vitro degradation and cytocompatibility of dicalcium phosphate dihydrate cements prepared using the monocalcium phosphate monohydrate/hydroxyapatite system reveals rapid conversion to HA as a key mechanism. J Biomed Mater Res B Appl Biomater 100B:595–602CrossRef
Zurück zum Zitat Andrade FK, Pértile RAN, Dourado F, Gama FMP (2010) Bacterial cellulose: properties, production and applications. In: Lejeune A, Deprez T (eds) Cellulose: structure and properties, derivatives and industrial uses. Nova Science Publishers, Hauppage, pp 427–458 Andrade FK, Pértile RAN, Dourado F, Gama FMP (2010) Bacterial cellulose: properties, production and applications. In: Lejeune A, Deprez T (eds) Cellulose: structure and properties, derivatives and industrial uses. Nova Science Publishers, Hauppage, pp 427–458
Zurück zum Zitat Andrade FK, Alexandre N, Amorim I, Gartner F, Maurício AC, Luís AL et al (2012) Studies on the biocompatibility of bacterial cellulose. J Bioact Compat Polym 28:97–112CrossRef Andrade FK, Alexandre N, Amorim I, Gartner F, Maurício AC, Luís AL et al (2012) Studies on the biocompatibility of bacterial cellulose. J Bioact Compat Polym 28:97–112CrossRef
Zurück zum Zitat Barrère F, Blitterswijk CA, Groot K (2006) Bone regeneration: molecular and cellular interactions with calcium phosphate ceramics. Int J Nanomed 1:317–332 Barrère F, Blitterswijk CA, Groot K (2006) Bone regeneration: molecular and cellular interactions with calcium phosphate ceramics. Int J Nanomed 1:317–332
Zurück zum Zitat Barud HS, Ribeiro C, Crespi M, Martines M, Dexpert-Ghys J, Marques R et al (2007) Thermal characterization of bacterial cellulose-phosphate composites membranes. J Therm Anal Calorim 87:815–818CrossRef Barud HS, Ribeiro C, Crespi M, Martines M, Dexpert-Ghys J, Marques R et al (2007) Thermal characterization of bacterial cellulose-phosphate composites membranes. J Therm Anal Calorim 87:815–818CrossRef
Zurück zum Zitat Barud HS, Assunção RMN, Martines MAU, Dexpert-Ghys J, Marques RFC, Messaddeq Y et al (2008a) Bacterial cellulose-silica organic-inorganic hybrids. J Sol Gel Sci Technol 46:363–367CrossRef Barud HS, Assunção RMN, Martines MAU, Dexpert-Ghys J, Marques RFC, Messaddeq Y et al (2008a) Bacterial cellulose-silica organic-inorganic hybrids. J Sol Gel Sci Technol 46:363–367CrossRef
Zurück zum Zitat Barud HS, de Araújo Júnior AM, Santos DB, de Assunção RMN, Meireles CS, Cerqueira DA et al (2008b) Thermal behavior of cellulose acetate produced from homogeneous acetylation of bacterial cellulose. Thermochim Acta 471:61–69CrossRef Barud HS, de Araújo Júnior AM, Santos DB, de Assunção RMN, Meireles CS, Cerqueira DA et al (2008b) Thermal behavior of cellulose acetate produced from homogeneous acetylation of bacterial cellulose. Thermochim Acta 471:61–69CrossRef
Zurück zum Zitat Basu B, Swain SK, Sarkar D (2013) Cryogenically cured hydroxyapatite-gelatin nanobiocomposite for bovine serum albumin protein adsorption and release. RSC Adv 3:14622–14633CrossRef Basu B, Swain SK, Sarkar D (2013) Cryogenically cured hydroxyapatite-gelatin nanobiocomposite for bovine serum albumin protein adsorption and release. RSC Adv 3:14622–14633CrossRef
Zurück zum Zitat Bohner M, Lemaitre J (2009) Can bioactivity be tested in vitro with SBF solution? Biomaterials 30:2175–2179CrossRef Bohner M, Lemaitre J (2009) Can bioactivity be tested in vitro with SBF solution? Biomaterials 30:2175–2179CrossRef
Zurück zum Zitat Bundela H, Bharadwaj V (2012) Synthesis and characterization of hydroxyapatite poly (vinyl alcohol) based nanocomposites for their perspective use as bone substitutes. Polym Sci Ser A 54:299–309CrossRef Bundela H, Bharadwaj V (2012) Synthesis and characterization of hydroxyapatite poly (vinyl alcohol) based nanocomposites for their perspective use as bone substitutes. Polym Sci Ser A 54:299–309CrossRef
Zurück zum Zitat Cavka A, Guo X, Shui-Jia T, Winestrand S, Jönsson LJ, Hong F (2013) Production of bacterial cellulose and enzyme from waste fiber sludge. Biotechnol Biofuels 6:1–10CrossRef Cavka A, Guo X, Shui-Jia T, Winestrand S, Jönsson LJ, Hong F (2013) Production of bacterial cellulose and enzyme from waste fiber sludge. Biotechnol Biofuels 6:1–10CrossRef
Zurück zum Zitat Chawla PR, Bajaj IB, Survase SA, Singhal RS (2009) Microbial cellulose: fermentative production and applications. Food Technol Biotechnol 47:107–124 Chawla PR, Bajaj IB, Survase SA, Singhal RS (2009) Microbial cellulose: fermentative production and applications. Food Technol Biotechnol 47:107–124
Zurück zum Zitat Coleman NJ, Nicholson JW, Awosanya K (2007) A preliminary investigation of the in vitro bioactivity of white Portland cement. Cement Concrete Res 37:1518–1523CrossRef Coleman NJ, Nicholson JW, Awosanya K (2007) A preliminary investigation of the in vitro bioactivity of white Portland cement. Cement Concrete Res 37:1518–1523CrossRef
Zurück zum Zitat Elliot JC (1994) Structure and chemistry of the apatite and other calcium orthophosphates, 1st edn. Elsevier, Amsterdam Elliot JC (1994) Structure and chemistry of the apatite and other calcium orthophosphates, 1st edn. Elsevier, Amsterdam
Zurück zum Zitat Fang B, Wan YZ, Tang TT, Gao C, Dai KR (2009) Proliferation and osteoblastic differentiation of human bone marrow stromal cells on hydroxyapatite/bacterial cellulose nanocomposite scaffolds. Tissue Eng Part A 15:1091–1098CrossRef Fang B, Wan YZ, Tang TT, Gao C, Dai KR (2009) Proliferation and osteoblastic differentiation of human bone marrow stromal cells on hydroxyapatite/bacterial cellulose nanocomposite scaffolds. Tissue Eng Part A 15:1091–1098CrossRef
Zurück zum Zitat Fricain JC, Granja PL, Barbosa MA, de Jeso B, Barthe N, Baquey C (2002) Cellulose phosphates as biomaterials. In vivo biocompatibility studies. Biomaterials 23:971–980CrossRef Fricain JC, Granja PL, Barbosa MA, de Jeso B, Barthe N, Baquey C (2002) Cellulose phosphates as biomaterials. In vivo biocompatibility studies. Biomaterials 23:971–980CrossRef
Zurück zum Zitat Giannoudis PV, Dinopoulos H, Tsiridis E (2005) Bone substitutes: an update. Injury 36:S20–S27CrossRef Giannoudis PV, Dinopoulos H, Tsiridis E (2005) Bone substitutes: an update. Injury 36:S20–S27CrossRef
Zurück zum Zitat Gomes FP, Silva NHCS, Trovatti E, Serafim LS, Duarte MF, Silvestre AJD et al (2013) Production of bacterial cellulose by Gluconacetobacter sacchari using dry olive mill residue. Biomass Bioenerg 55:205–211CrossRef Gomes FP, Silva NHCS, Trovatti E, Serafim LS, Duarte MF, Silvestre AJD et al (2013) Production of bacterial cellulose by Gluconacetobacter sacchari using dry olive mill residue. Biomass Bioenerg 55:205–211CrossRef
Zurück zum Zitat Grande CJ, Torres FG, Gomez CM, Carmen Baño MC (2009) Nanocomposites of bacterial cellulose/hydroxyapatite for biomedical applications. Acta Biomater 5:1605–1615CrossRef Grande CJ, Torres FG, Gomez CM, Carmen Baño MC (2009) Nanocomposites of bacterial cellulose/hydroxyapatite for biomedical applications. Acta Biomater 5:1605–1615CrossRef
Zurück zum Zitat He M, Chang C, Peng N, Zhang L (2012) Structure and properties of hydroxyapatite/cellulose nanocomposite films. Carbohydr Polym 87:2512–2518CrossRef He M, Chang C, Peng N, Zhang L (2012) Structure and properties of hydroxyapatite/cellulose nanocomposite films. Carbohydr Polym 87:2512–2518CrossRef
Zurück zum Zitat Helenius G, Backdahl H, Bodin A, Nannmark U, Gatenholm P, Risberg B (2006) In vivo biocompatibility of bacterial cellulose. J Biomed Mater Res A 76A:431–438CrossRef Helenius G, Backdahl H, Bodin A, Nannmark U, Gatenholm P, Risberg B (2006) In vivo biocompatibility of bacterial cellulose. J Biomed Mater Res A 76A:431–438CrossRef
Zurück zum Zitat Hestrin S, Schramm M (1954) Synthesis of cellulose by acetobacter-xylinum.2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 58:345–352CrossRef Hestrin S, Schramm M (1954) Synthesis of cellulose by acetobacter-xylinum.2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 58:345–352CrossRef
Zurück zum Zitat Huang Y, Zhu C, Yang J, Nie Y, Chen C, Sun D (2014) Recent advances in bacterial cellulose. Cellulose 21:1–30CrossRef Huang Y, Zhu C, Yang J, Nie Y, Chen C, Sun D (2014) Recent advances in bacterial cellulose. Cellulose 21:1–30CrossRef
Zurück zum Zitat Hutchens SA, Benson RS, Evans BR, O’Neill HM, Rawn CJ (2006) Biomimetic synthesis of calcium-deficient hydroxyapatite in a natural hydrogel. Biomaterials 27:4661–4670CrossRef Hutchens SA, Benson RS, Evans BR, O’Neill HM, Rawn CJ (2006) Biomimetic synthesis of calcium-deficient hydroxyapatite in a natural hydrogel. Biomaterials 27:4661–4670CrossRef
Zurück zum Zitat Janicki P, Schmidmaier G (2011) What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells. Injury 42:S77–S81CrossRef Janicki P, Schmidmaier G (2011) What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells. Injury 42:S77–S81CrossRef
Zurück zum Zitat Kim HW, Knowles JC, Kim HE (2004) Hydroxyapatite/poly(e-caprolactone) composite coatings on hydroxyapatite porous bone scaffold for drug delivery. Biomaterials 25:1279–1287CrossRef Kim HW, Knowles JC, Kim HE (2004) Hydroxyapatite/poly(e-caprolactone) composite coatings on hydroxyapatite porous bone scaffold for drug delivery. Biomaterials 25:1279–1287CrossRef
Zurück zum Zitat Landi E, Tampieri A, Celotti G, Sprio S (2000) Densification behaviour and mechanisms of synthetic hydroxyapatites. J Eur Ceram Soc 20:2377–2387CrossRef Landi E, Tampieri A, Celotti G, Sprio S (2000) Densification behaviour and mechanisms of synthetic hydroxyapatites. J Eur Ceram Soc 20:2377–2387CrossRef
Zurück zum Zitat Le Guéhennec L, Layrolle P, Daculsi G (2004) A review of bioceramics and fibrin sealant. Eur Cell Mater 8:1–10 Le Guéhennec L, Layrolle P, Daculsi G (2004) A review of bioceramics and fibrin sealant. Eur Cell Mater 8:1–10
Zurück zum Zitat Mohamed KR (2012). Biocomposite materials, composites and their applications. In: Prof. Ning H (ed.) InTech, pp 113–146 Mohamed KR (2012). Biocomposite materials, composites and their applications. In: Prof. Ning H (ed.) InTech, pp 113–146
Zurück zum Zitat Moore WR, Graves SE, Bain GI (2001) Synthetic bone graft substitutes. ANZ J Surg 71:354–361CrossRef Moore WR, Graves SE, Bain GI (2001) Synthetic bone graft substitutes. ANZ J Surg 71:354–361CrossRef
Zurück zum Zitat Morejón-Alonso L, Carrodeguas RG, García-Menocal JAD, Pérez JAA, Manent SM (2007) Effect of sterilization on the properties of CDHA-OCP-β-TCP biomaterial. Mater Res 10:15–20CrossRef Morejón-Alonso L, Carrodeguas RG, García-Menocal JAD, Pérez JAA, Manent SM (2007) Effect of sterilization on the properties of CDHA-OCP-β-TCP biomaterial. Mater Res 10:15–20CrossRef
Zurück zum Zitat Morejón-Alonso L, Carrodeguas RG, García-Menocal JAD (2008) Transformations in CDHA/OCP/β-TCP scaffold during ageing in simulated body fluid at 36.5 °C. J Biomed Mater Res B Appl Biomater 84B:386–393CrossRef Morejón-Alonso L, Carrodeguas RG, García-Menocal JAD (2008) Transformations in CDHA/OCP/β-TCP scaffold during ageing in simulated body fluid at 36.5 °C. J Biomed Mater Res B Appl Biomater 84B:386–393CrossRef
Zurück zum Zitat Nge TT, Sugiyama J, Bulone V (2010) Bacterial cellulose-based biomimetic composites. In: Elnashar M (ed) Biopolymers. InTech, Rijeka, pp 345–368 Nge TT, Sugiyama J, Bulone V (2010) Bacterial cellulose-based biomimetic composites. In: Elnashar M (ed) Biopolymers. InTech, Rijeka, pp 345–368
Zurück zum Zitat Pértile RA, Moreira S, Costa RM, Correia A, Guardao L, Gartner F et al (2012) Bacterial cellulose: long-term biocompatibility studies. J Biomater Sci Polym 23:1339–1354 Pértile RA, Moreira S, Costa RM, Correia A, Guardao L, Gartner F et al (2012) Bacterial cellulose: long-term biocompatibility studies. J Biomater Sci Polym 23:1339–1354
Zurück zum Zitat Rivas B, Moldes AB, Dominguez JM, Parajo JC (2004) Development of culture media containing spent yeast cells of Debaryomyces hansenii and corn steep liquor for lactic acid production with Lactobacillus rhamnosus. Int J Food Microbiol 97:93–98CrossRef Rivas B, Moldes AB, Dominguez JM, Parajo JC (2004) Development of culture media containing spent yeast cells of Debaryomyces hansenii and corn steep liquor for lactic acid production with Lactobacillus rhamnosus. Int J Food Microbiol 97:93–98CrossRef
Zurück zum Zitat Roveri N, Foresti E, Lelli M, Lesci IG, Marchetti M (2010) Microscopic investigations of synthetic biomimetic hydroxyapatite. Microsc Sci Technol Appl Educ 3:1868–1879 Roveri N, Foresti E, Lelli M, Lesci IG, Marchetti M (2010) Microscopic investigations of synthetic biomimetic hydroxyapatite. Microsc Sci Technol Appl Educ 3:1868–1879
Zurück zum Zitat Saska S, Barud HS, Gaspar AMM, Marchetto R, Ribeiro SJL, Messaddeq Y (2011) Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration. Int J Biomater 2011:1–8CrossRef Saska S, Barud HS, Gaspar AMM, Marchetto R, Ribeiro SJL, Messaddeq Y (2011) Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration. Int J Biomater 2011:1–8CrossRef
Zurück zum Zitat Schumann DA, Wippermann J, Klemm DO, Kramer F, Koth D, Kosmehl H et al (2009) Artificial vascular implants from bacterial cellulose: preliminary results of small arterial substitutes. Cellulose 16:877–885CrossRef Schumann DA, Wippermann J, Klemm DO, Kramer F, Koth D, Kosmehl H et al (2009) Artificial vascular implants from bacterial cellulose: preliminary results of small arterial substitutes. Cellulose 16:877–885CrossRef
Zurück zum Zitat Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794CrossRef Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794CrossRef
Zurück zum Zitat Suzuki O (2010) Octacalcium phosphate: osteoconductivity and crystal chemistry. Acta Biomater 6:3379–3387CrossRef Suzuki O (2010) Octacalcium phosphate: osteoconductivity and crystal chemistry. Acta Biomater 6:3379–3387CrossRef
Zurück zum Zitat Swain SK, Sarkar D (2013) Study of BSA protein adsorption/release on hydroxyapatite nanoparticles. Appl Surf Sci 286:99–103CrossRef Swain SK, Sarkar D (2013) Study of BSA protein adsorption/release on hydroxyapatite nanoparticles. Appl Surf Sci 286:99–103CrossRef
Zurück zum Zitat Uesu NY, Pineda EA, Hechenleitner AA (2000) Microcrystalline cellulose from soybean husk: effects of solvent treatments on its properties as acetylsalicylic acid carrier. Int J Pharm 206:85–96CrossRef Uesu NY, Pineda EA, Hechenleitner AA (2000) Microcrystalline cellulose from soybean husk: effects of solvent treatments on its properties as acetylsalicylic acid carrier. Int J Pharm 206:85–96CrossRef
Zurück zum Zitat Wan YZ, Huang Y, Yuan CD, Raman S, Zhu Y, Jiang HJ et al (2007) Biomimetic synthesis of hydroxyapatite/bacterial cellulose nanocomposites for biomedical applications. Mat Sci Eng C Bio S 27:855–864CrossRef Wan YZ, Huang Y, Yuan CD, Raman S, Zhu Y, Jiang HJ et al (2007) Biomimetic synthesis of hydroxyapatite/bacterial cellulose nanocomposites for biomedical applications. Mat Sci Eng C Bio S 27:855–864CrossRef
Zurück zum Zitat Wang K, Zhou C, Hong Y, Zhang X (2012) A review of protein adsorption on bioceramics. Interface Focus 2:259–277CrossRef Wang K, Zhou C, Hong Y, Zhang X (2012) A review of protein adsorption on bioceramics. Interface Focus 2:259–277CrossRef
Zurück zum Zitat Wippermann J, Schumann D, Klemm D, Kosmehl H, Salehi-Gelani S, Wahlers T (2009) Preliminary results of small arterial substitute performed with a new cylindrical biomaterial composed of bacterial cellulose. Eur J Vasc Endovasc Surg 37:592–598CrossRef Wippermann J, Schumann D, Klemm D, Kosmehl H, Salehi-Gelani S, Wahlers T (2009) Preliminary results of small arterial substitute performed with a new cylindrical biomaterial composed of bacterial cellulose. Eur J Vasc Endovasc Surg 37:592–598CrossRef
Zurück zum Zitat Wu JM, Liu RH (2013) Cost-effective production of bacterial cellulose in static cultures using distillery wastewater. J Biosci Bioeng 115:284–290CrossRef Wu JM, Liu RH (2013) Cost-effective production of bacterial cellulose in static cultures using distillery wastewater. J Biosci Bioeng 115:284–290CrossRef
Zurück zum Zitat Zadegan S, Hosainalipour M, Rezaie HR, Ghassai H, Shokrgozar MA (2011) Synthesis and biocompatibility evaluation of cellulose/hydroxyapatite nanocomposite scaffold in 1- n-allyl-3-methylimidazolium chloride. Mater Sci Eng C 31:954–961CrossRef Zadegan S, Hosainalipour M, Rezaie HR, Ghassai H, Shokrgozar MA (2011) Synthesis and biocompatibility evaluation of cellulose/hydroxyapatite nanocomposite scaffold in 1- n-allyl-3-methylimidazolium chloride. Mater Sci Eng C 31:954–961CrossRef
Zurück zum Zitat Zhai Y, Cui FZ, Wang Y (2005) Formation of nano-hydroxyapatite on recombinant human-like collagen fibrils. Curr Appl Phys 5:429–432CrossRef Zhai Y, Cui FZ, Wang Y (2005) Formation of nano-hydroxyapatite on recombinant human-like collagen fibrils. Curr Appl Phys 5:429–432CrossRef
Zurück zum Zitat Zimmermann KA, LeBlanc JM, Sheets KT, Fox RW, Gatenholm P (2011) Biomimetic design of a bacterial cellulose/hydroxyapatite nanocomposite for bone healing applications. Mater Sci Eng C 31:43–49CrossRef Zimmermann KA, LeBlanc JM, Sheets KT, Fox RW, Gatenholm P (2011) Biomimetic design of a bacterial cellulose/hydroxyapatite nanocomposite for bone healing applications. Mater Sci Eng C 31:43–49CrossRef
Metadaten
Titel
Production of hydroxyapatite–bacterial cellulose nanocomposites from agroindustrial wastes
verfasst von
Eden B. Duarte
Bruna S. das Chagas
Fábia K. Andrade
Ana I. S. Brígida
Maria F. Borges
Celli R. Muniz
Men de Sá M. Souza Filho
João P. S. Morais
Judith P. A. Feitosa
Morsyleide F. Rosa
Publikationsdatum
01.10.2015
Verlag
Springer Netherlands
Erschienen in
Cellulose / Ausgabe 5/2015
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-015-0734-8

Weitere Artikel der Ausgabe 5/2015

Cellulose 5/2015 Zur Ausgabe