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Erschienen in: Cellulose 4/2015

01.08.2015 | Original Paper

Research on degradation of cellulose using enzyme treatment in flue-cured tobacco by 13C NMR spectroscopy

verfasst von: Xiaolan Zhu, Lanlan Tan, Guanglin Feng, Jun Yang, Yun Gao, Ya Dai

Erschienen in: Cellulose | Ausgabe 4/2015

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Abstract

Cellulase (CE) and polygalacturonase (PG) were used to treat flue-cured tobacco, and then the content and structure of cellulose in tobacco were determined by 13C NMR spectra. Neutral aroma substances in tobacco were also determined by the GC-MS method. The results showed that enzyme treatment could decompose cellulose and other cell wall substances in tobacco effectively, and the highest decrease degree for cellulose was 13.5 %. The crystalline allomorph analysis showed that enzyme treatment would lead to a decrease in the ratio of Iα and increase in Iβ; however, the paracrystalline ratio increased obviously after CE treatment but decreased after PG treatment. There was a significant increase in the amount of total neutral aroma substances, which increased by 38.7 % at a CE concentration of 20 g l−1 compared to the control, while the PG treatment showed as much as that of the control.

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Metadaten
Titel
Research on degradation of cellulose using enzyme treatment in flue-cured tobacco by 13C NMR spectroscopy
verfasst von
Xiaolan Zhu
Lanlan Tan
Guanglin Feng
Jun Yang
Yun Gao
Ya Dai
Publikationsdatum
01.08.2015
Verlag
Springer Netherlands
Erschienen in
Cellulose / Ausgabe 4/2015
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-015-0676-1

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