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01.12.2014 | Original Research | Ausgabe 4/2014 Open Access

International Journal of Energy and Environmental Engineering 4/2014

Screening and optimization of parameters affecting fungal pretreatment of oil palm empty fruit bunch (EFB) by experimental design

Zeitschrift:
International Journal of Energy and Environmental Engineering > Ausgabe 4/2014
Autoren:
Agarat Kamcharoen, Verawat Champreda, Lily Eurwilaichitr, Piyarat Boonsawang

Abstract

In the present study, various white-rot fungi were used for the pretreatment of oil palm empty fruit bunch (EFB) using solid-state cultivation. The results showed that Trametes versicolor TISTR 3224 gave the highest selectivity value (the ratio of lignin degradation to cellulose degradation) of 1.57. In comparison, Trametes sp. BCC 8729, Phanerochaete chrysosporium ATCC 24725, Marasmius sp. BCC 9542 and Xylaria sp. BCC 7749 gave selectivity of 0.60, 0.59, 0.30 and 0.06, respectively. Screening parameters for the fungal pretreatment of EFB using T. versicolor TISTR 3224 was studied by Plackett–Burman design (PBD). It indicated that the moisture content and co-substrate gave a positive effect on the lignin degradation, while EFB concentration had a negative effect on cellulose degradation. The optimum conditions for lignin degradation obtained from Box–Behnken statistical experimental design (BBD) were 80 % moisture content, 2.29 % wheat flour and 23.3 % EFB. Under this condition, 15.6 % of delignification was obtained. After an enzymatic hydrolysis, the digestibility of fungal treated EFB under the optimum condition achieved 1.34-fold compared with untreated EFB.
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